Volumetric muscle loss injury repair using in situ fibrin gel cast seeded with muscle-derived stem cells (MDSCs)

Matthias, Nadine; Hunt, Samuel D.; Wu, Jianbo; Lo, Jonathan; Callahan, Laura A. Smith; Li, Yong; Huard, Johnny; Darabi, Radbod

doi:10.1016/j.scr.2018.01.008

Cited by 65 publications

(55 citation statements)

References 38 publications

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“…140 Another study evaluated the efficacy of muscle-derived stem cells (MDSCs) combined with fibrin gel for VML repair in immunodeficient mice. 134 At 4 weeks post-injury, this combined approach showed that MDSCs differentiated into new myofibers and significantly increased muscle mass along with a significant decrease in fibrotic tissue deposition. The number of small fibers with centrally located nuclei were reported to increase with treatment indicating significant increase of regenerative fibers in the engrafted area of treated muscles (Fig.…”

Section: Satellite Cells and Myoblasts Transplantation With Biomaterialsmentioning

confidence: 91%

“…Combining cells with candidate biomaterials may overcome issues associated with acellular scaffolds and cell‐only therapies. Such combination therapies have shown promising results in animal models and may provide a potential therapy in the near future . The following section describe some of the promising scaffold technology that has been developed in combination with cellular therapies over the recent years.…”

Section: Biomaterials For Skeletal Muscle Regenerationmentioning

confidence: 99%

“…Another study evaluated the efficacy of muscle‐derived stem cells (MDSCs) combined with fibrin gel for VML repair in immunodeficient mice . At 4 weeks post‐injury, this combined approach showed that MDSCs differentiated into new myofibers and significantly increased muscle mass along with a significant decrease in fibrotic tissue deposition.…”

Section: Biomaterials For Skeletal Muscle Regenerationmentioning

confidence: 99%

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Biomaterial and stem cell‐based strategies for skeletal muscle regeneration

Dunn

Talovic

Patel

et al. 2019

Journal Orthopaedic Research

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Adult skeletal muscle can regenerate effectively after mild physical or chemical insult. Muscle trauma or disease can overwhelm this innate capacity for regeneration and result in heightened inflammation and fibrotic tissue deposition resulting in loss of structure and function. Recent studies have focused on biomaterial and stem cell‐based therapies to promote skeletal muscle regeneration following injury and disease. Many stem cell populations besides satellite cells are implicated in muscle regeneration. These stem cells include but are not limited to mesenchymal stem cells, adipose‐derived stem cells, hematopoietic stem cells, pericytes, fibroadipogenic progenitors, side population cells, and CD133+ stem cells. However, several challenges associated with their isolation, availability, delivery, survival, engraftment, and differentiation have been reported in recent studies. While acellular scaffolds offer a relatively safe and potentially off‐the‐shelf solution to cell‐based therapies, they are often unable to stimulate host cell migration and activity to a level that would result in clinically meaningful regeneration of traumatized muscle. Combining stem cells and biomaterials may offer a viable therapeutic strategy that may overcome the limitations associated with these therapies when they are used in isolation. In this article, we review the stem cell populations that can stimulate muscle regeneration in vitro and in vivo. We also discuss the regenerative potential of combination therapies that utilize both stem cell and biomaterials for the treatment of skeletal muscle injury and disease. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1246–1262, 2019.

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Section: Satellite Cells and Myoblasts Transplantation With Biomaterialsmentioning

confidence: 91%

Section: Biomaterials For Skeletal Muscle Regenerationmentioning

confidence: 99%

Section: Biomaterials For Skeletal Muscle Regenerationmentioning

confidence: 99%

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Biomaterial and stem cell‐based strategies for skeletal muscle regeneration

Dunn

Talovic

Patel

et al. 2019

Journal Orthopaedic Research

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“…Konigsberg long pointed out the importance of the extracellular matrix (ECM) protein collagen as a critical component to the development of muscle colonies, which led to its widespread use in SMTE . Since then, other natural and synthetic materials such as fibrin, alginate, polycaprolactone (PCL)‐based polymers, and various strategies have been developed to generate skeletal muscle tissues in vitro. Especially, the engineering of muscle fibers in vitro requires the culture of myoblasts in an anisotropic environment, promoting their alignment, favoring their fusion and the myogenesis .…”

Section: Skeletal Muscle Tissue Engineeringmentioning

confidence: 99%

3D Bioprinting in Skeletal Muscle Tissue Engineering

Ostrovidov

Salehi

Costantini

et al. 2019

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230

166

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Skeletal muscle tissue engineering (SMTE) aims at repairing defective skeletal muscles. Until now, numerous developments are made in SMTE; however, it is still challenging to recapitulate the complexity of muscles with current methods of fabrication. Here, after a brief description of the anatomy of skeletal muscle and a short state‐of‐the‐art on developments made in SMTE with “conventional methods,” the use of 3D bioprinting as a new tool for SMTE is in focus. The current bioprinting methods are discussed, and an overview of the bioink formulations and properties used in 3D bioprinting is provided. Finally, different advances made in SMTE by 3D bioprinting are highlighted, and future needs and a short perspective are provided.

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“…To expose the tibialis anterior muscle, a longitudinal incision was made, followed by the creation of a wedge-shaped defect of 6 mm × 3 mm × 4 mm in length, width, and depth. For in situ casting of the MDSCs with fibrin gel, freshly harvested MDSCs (1 × 10 6 ) were re-suspended in 20 μl of fibrinogen solution (MedChemExpress) with or without 100 nM of SW033291, and the muscle defect was filled with the solution followed by addition of thrombin solution (5 IU/2 μl, MedChemExpress) to induce gel casting [42]. After 2 min waiting period to ensure gel solidification, the wound was carefully covered with the fascia and skin and secured using sutures.…”

Section: Surgical Procedures and Gel Castingmentioning

confidence: 99%

Effects of SW033291 on the myogenesis of muscle-derived stem cells and muscle regeneration

Dong

Zhang

et al. 2020

Stem Cell Res Ther

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Background: The unmet medical needs in repairing large muscle defects promote the development of tissue regeneration strategy. The use of bioactive molecules in combination with biomaterial scaffold has become an area of great interest. SW033291, a small-molecule inhibitor targeting 15-hydroxyprostaglandin dehydrogenase (15-PDGH) and subsequently elevating the production of prostaglandin E2 (PGE2), has been proved to accelerate the recovery and potentiate the regeneration of multiple tissues including the bone, liver, and colon. The limited understanding of the potential therapeutic effects on myogenesis motivated us to investigate the role of SW033291 in regulating muscle-derived stem cell (MDSC) myogenic differentiation and MDSC-mediated muscle regeneration. Methods: The characteristics of rat MDSCs, including cell-specific markers and myogenic differentiation potential, were determined. MDSCs were incubated with SW033291 to evaluate PGE2 production and cytotoxicity. The effects of SW033291 on MDSC myogenic differentiation were assessed by quantitative real-time polymerase chain reaction (qPCR), western blot, and immunocytochemistry. The fibrin gel containing MDSCs and SW033291 was used for muscle regeneration in a tibialis anterior muscle defect model. Results: Our data demonstrated that MDSCs were well-tolerated to SW033291 and treatment with SW033291 significantly promoted the production of PGE2 by MDSCs. In vitro analysis showed that SW033291 enhanced the myogenic differentiation and myotube formation by upregulating a series of myogenic markers. Additionally, the activation of PI3K/Akt pathway was involved in the mechanism underlying these promotive effects. Then, in situ casting of fibrin gel containing MDSCs and SW033291 was used to repair the tibialis anterior muscle defect; the addition of SW033291 significantly promoted myofiber formation within the defect region with mild immune response, less fibrosis, and sufficient vascularization. Conclusion: SW033291 acted as a positive regulator of MDSC myogenic differentiation, and incorporating the compound with MDSCs in fibrin gel could serve as an effective method to repair large skeletal muscle defects.

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Volumetric muscle loss injury repair using in situ fibrin gel cast seeded with muscle-derived stem cells (MDSCs)

Cited by 65 publications

References 38 publications

Biomaterial and stem cell‐based strategies for skeletal muscle regeneration

Biomaterial and stem cell‐based strategies for skeletal muscle regeneration

3D Bioprinting in Skeletal Muscle Tissue Engineering

Effects of SW033291 on the myogenesis of muscle-derived stem cells and muscle regeneration

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