Von Willebrand Disease: Range of the Disease, and Management

Distinct platelet F-actin patterns and traction forces on von Willebrand factor versus fibrinogen

“…On the day of the platelet experiment, human VWF (Haematologic Technologies) or human fibrinogen (Sigma) was incubated on ethanol-cleaned glass coverslips or black dots substrates for 1 to 2 hours. VWF and fibrinogen were respectively diluted to 5 µg/mL and 1 mg/mL because these are the approximate concentrations in human blood (i.e., approximately half of the concentration of each in plasma) (29,30). Glass coverslips were then blocked with 1% bovine serum albumin (Sigma) for 2 hours.…”