Voriconazole loaded nanostructured lipid carriers based topical delivery system: QbD based designing, characterization, in-vitro and ex-vivo evaluation

Waghule, Tejashree; Rapalli, Vamshi Krishna; Singhvi, Gautam; Manchanda, Prachi; Hans, Neha; Dubey, Sunil Kumar; Hasnain, Saquib; Nayak, Amit Kumar

doi:10.1016/j.jddst.2019.04.026

Cited by 104 publications

(44 citation statements)

References 39 publications

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“…Drug concentration was calculated from the calibration curve equation. Further, entrapment efficiency and drug loading were calculated using the following equations .

\begin{matrix} true \\ left % Entrapment efficiency \\ left = \frac{Amount of drug in nanocarriers}{Amount of drug used in formulation} \times 100 \end{matrix}

\begin{matrix} true \\ left % Drug loading \\ left = \frac{Amount of drug in nanocarriers}{Weight of nanocarriers} \times 100 \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

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Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Rapalli

Singhvi

Gorantla

et al. 2019

J of Separation Science

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Low‐potency corticosteroid betamethasone valerate and vitamin‐A tazarotene are used in combination for effective treatment of psoriasis. There is no robust high‐performance liquid chromatography analytical technique available for simultaneous estimation of betamethasone valerate and tazarotene in conventional and nanocarriers based formulations. A simple, accurate, robust isocratic high‐performance liquid chromatography method was developed for simultaneous estimation of betamethasone valerate and tazarotene in topical pharmaceutical formulations. The developed method was validated as per the regulatory guidelines. The validated method was linear over the concentration range of 150–6000 ng/mL (r2 > 0.999) at 239 nm wavelength. Limits of detection and quantification of two analytes were 50 and 150 ng/mL, respectively. The %relative standard deviation for intraday and interday precision was less than 2%. The method was also evaluated in the presence of forced degradation conditions. The developed method was successfully applied for in vitro and ex vivo drug release studies of in‐house designed nanoformulations.

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“…Drug concentration was calculated from the calibration curve equation. Further, entrapment efficiency and drug loading were calculated using the following equations .

\begin{matrix} true \\ left % Entrapment efficiency \\ left = \frac{Amount of drug in nanocarriers}{Amount of drug used in formulation} \times 100 \end{matrix}

\begin{matrix} true \\ left % Drug loading \\ left = \frac{Amount of drug in nanocarriers}{Weight of nanocarriers} \times 100 \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

“…Drug concentration was calculated from the calibration curve equation. Further, entrapment efficiency and drug loading were calculated using the following equations [31].…”

Section: Entrapment Efficiency and Drug Loadingmentioning

confidence: 99%

Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Rapalli

Singhvi

Gorantla

et al. 2019

J of Separation Science

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“…NLCs 22 Voriconazole Improved permeation (66.45%), sustained release (11 h), antifungal studies showed 1.5 times higher zone of inhibition as compared to free drug. index as compared to the marketed Candistan cream.…”

Section: Slns 21 Fluconazolementioning

confidence: 99%

Emerging role of nanocarriers based topical delivery of anti‐fungal agents in combating growing fungal infections

Waghule

Sankar

Rapalli

et al. 2020

Dermatologic Therapy

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The incidences of fungal infections have greatly increased over the past few years, particularly in humid and industrialized areas. The severity of such infections ranges from being asymptomatic-mild to potentially life-threatening systemic infections. There are limited classes of drugs that are approved for the treatment of such infections like polyenes, azoles, and echinocandins. Some fungi have developed resistance to these drugs. Therefore, to counter drug resistance, intensive large scale studies on novel targeting strategies and formulations are being conducted, which have gained impetus lately. Conventional formulations have limitations such as higher doses, frequent dosing, and several side effects. Such limiting factors have paved the path for the emergence of nanotechnology and its applications. This further gave formulation scientists the possibility of encapsulating the existing potential drug moieties into nanocarriers, which when loaded into gels or creams provided prolonged release and improved permeation, thus giving on-target effect. This review thus discusses the newer targeting strategies and the role of nanocarriers that could be administered topically for the treatment of various fungal infections. Furthermore, this approach opens newer avenues for continued and sustained research in pharmaceuticals with much more effective outcomes.

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“…In the field of material science, nanotechnology is unremittingly carving its own zone as an emerging field of research. Applicability of nanoparticles (NPs) and nanomaterials is evolving rapidly; specially on the biomedical front from cell imaging, targeted drug delivery and cancer diagnostics to therapeutic applications [1][2][3][4][5][6][7][8]. Both the physical and chemical methods, or either of any, can be significantly applied for the synthesis of NPs [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Study of Reaction Kinetics. The effect of varying temperatures (25°C-40°C), pH(5)(6)(7)(8)(9), agitation speed (50-250 rpm), and HAuCl 4 concentration (1-10 mg/L) over the reaction kinetics involved in the biosynthesis of AuNPs was attempted to comprehend in this study. Multiple sets of experiments were carried out by varying one factor at a time (OFAT) while keeping the other factors constant over a period of reaction time (0-48 h) intervals.…”

mentioning

confidence: 99%

Crystal Growth and Kinetic Behaviour of Pseudoalteromonas espejiana Assisted Biosynthesized Gold Nanoparticles

Kumar

Das

Alkahtani

et al. 2020

Oxidative Medicine and Cellular Longevity

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Pseudoalteromonas espejiana (P. espejiana) is a marine bacterium known for its high resistance to alkalinity. The ability of P. espejiana to reduce Au (III) and biosynthesize gold nanoparticles (AuNPs) is found positive and was confirmed using UV-VIS, EDS, SEM, and TEM studies. Previously, many studies have been reported regarding the crystalline nature of AuNPs; therefore, this research aims at studying the crystal growth behaviour of AuNPs through DLS and TEM studies. Spherically shaped and monodispersed, AuNPs ranging between 5 to 160 nm were obtained with an average particle size of 62 nm. Also, to achieve maximum production of AuNPs, the reaction kinetic study was performed using an ICP-OES method and the effect of various parameters including pH, temperature, rpm, and concentration of substrate was analyzed. During the biosynthesis process, an appropriate phase of nucleation, crystal growth, and saturation was observed and this helped to determine the rate constants and order of reaction. The parameters such as pH profile (pH 9), temperature (30°C), agitation speed (150 rpm), and enzyme substrate ratio (2 : 3) were found to be the best fits for maximum production of low size AuNPs. This demonstrates that in initial few hours, a quick conversion of the ionic gold precursor takes place into metallic gold nuclei, trailed by crystal growth via coalescence of small nuclei. Subsequently, it can be concluded that coalescence processes drive the crystal growth process of AuNPs over a time interval and finally leads to saturation and no newer particle formation in the solution.

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Voriconazole loaded nanostructured lipid carriers based topical delivery system: QbD based designing, characterization, in-vitro and ex-vivo evaluation

Cited by 104 publications

References 39 publications

Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Stability indicating liquid chromatographic method for simultaneous quantification of betamethasone valerate and tazarotene in in vitro and ex vivo studies of complex nanoformulation

Emerging role of nanocarriers based topical delivery of anti‐fungal agents in combating growing fungal infections

Crystal Growth and Kinetic Behaviour of Pseudoalteromonas espejiana Assisted Biosynthesized Gold Nanoparticles

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