Vox Sanguinis International Forum on platelet cryopreservation

Cohn, Claudia S.; Dumont, Larry J.; Lozano, Miquel; Marks, Denese C.; Johnson, Lacey; Ismay, S.; Bondar, N.; T'Sas, F.; Yokoyama, Ana Paula Hitomi; Kutner, José Mauro; Acker, Jason P.; Bohoněk, Miloš; Sailliol, A.; Martinaud, Christophe; Pogłód, Ryszard; Antoniewicz‐Papis, Jolanta; Lachert, Elżbieta; Pun, Pamela Boon Li; Lu, Jia; Cid, Joan; Guijarro, Francesca; Puig, Lluís; Gerber, Bernhard; Alberio, Lorenzo; Schanz, Urs; Buser, Andreas; Noorman, Femke; Zoodsma, Margreet; Meer, Pieter F. van der; Korte, Dirk de; Wagner, Stephen J.; O'Neill, M.

doi:10.1111/vox.12532

Cited by 23 publications

(30 citation statements)

References 25 publications

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“…Platelet cryopreservation is an attractive alternative to RT storage, and international interest in this storage option has grown considerably in the past decade . For cryopreservation, 5% to 6% dimethyl sulfoxide (DMSO) is added to the platelets and they are frozen and stored at −80°C, which extends their shelf life to at least 2 years .…”

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confidence: 99%

“…Freezing platelets within 2 days of collection reduces the available inventory of liquid‐stored platelets and, combined with unpredictable supply and demand, may actually result in the inability to meet demand for this component. As the clinical indications for liquid‐stored and cryopreserved platelets (CPPs) are likely to be distinct, a flexible approach to the management of these inventories would be beneficial. Potentially, if platelets could be cryopreserved later in their usable shelf life, following storage at RT, this would maximize utility, as they could be available for standard transfusion and then frozen if not required.…”

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confidence: 99%

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Freezing expired platelets does not compromise in vitro quality: An opportunity to maximize inventory potential

et al. 2019

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BACKGROUND AND OBJECTIVES Cryopreservation provides an option for long‐term storage of platelet concentrates. While platelets are usually frozen as soon as practical after collection (within 2 days), the ability to freeze units at a later stage of the shelf life may improve inventory management. As such, the aim of this study was to determine the impact of freezing platelets approaching expiry (Day 5/6). MATERIALS AND METHODS Two ABO‐matched buffy coat–derived platelets (30% plasma/70% platelet additive solution) were pooled and split to produce matched pairs (n = 8 pairs). Platelets were frozen on Day 1 after collection (cryopreserved platelets [CPPs]) or Day 5 or 6 (expired‐CPPs) at −80°C with 5% to 6% dimethyl sulfoxide. In vitro platelet quality was tested before freezing and after thawing and reconstitution in plasma. RESULTS The majority of prefreeze parameters were equivalent for all platelet units (Day 1 vs. Day 5 or 6). Expired‐CPPs had a higher mean postthaw platelet recovery (82 ± 4%) compared to CPPs (75 ± 4%; p = 0.0021). Cryopreservation resulted in a loss of surface glycoproteins (glycoprotein (GP) Ibα, GPIIb, GPVI), an increase in activation markers (phosphatidylserine and P‐selectin) and microparticle release, compared to unfrozen platelets. However, the cryopreservation‐induced changes were equivalent in CPPs and expired‐CPPs. Functionality was measured by thromboelastography and was similar between expired‐CPPs (R‐time: 5.3 ± 0.3) and CPPs (R‐time: 5.4 ± 0.5; p = 0.7094). CONCLUSION The phenotype and functional profile of platelets frozen at expiry were similar to platelets frozen 1 day following collection. These data suggest that expired platelets may represent a suitable starting material for cryopreservation.

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confidence: 99%

Freezing expired platelets does not compromise in vitro quality: An opportunity to maximize inventory potential

et al. 2019

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“…To date, CPPs have been deployed in a number of military missions and confirmed to be effective in treating bleeding casualties, but have not been authorized for use in civilians in any country, with the main concern being the safety of CPPs for transfusion. Controlled clinical trials to demonstrate safety and efficacy are under way in several countries, but definitive results have not yet been obtained . Over the past 40 years, the cellular mechanisms of coagulation and thrombin generation have been widely investigated and are helpful for understanding the efficacy of CPPs, but reports on the mechanism of adverse reactions to CPP transfusion are scarce.…”

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confidence: 99%

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Zhao

Gan

et al. 2019

Transfusion

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BACKGROUND Cryopreservation in dimethyl sulfoxide and storage at −80 °C extends the shelf life of platelets to at least 2 years, allowing greater availability in rural and military areas. While cryopreserved platelets (CPPs) have been extensively characterized for coagulation and thrombin generation, reports on the mechanism of adverse reactions to CPPs transfusion are scarce. Here, we tested the hypothesis that CPPs facilitate phagocytosis by Kupffer cells and subsequently promote the inflammatory response in Kupffer cells. STUDY DESIGN AND METHODS P‐selectin expression, glycoprotein Ibα clustering and phosphatidylserine (PS) surface exposure on platelets stored at 22 °C, 4 °C and − 80 °C for 3 days were examined by flow cytometry. The phagocytosis of mepacrine‐labeled platelets coincubated with THP‐1 cells was examined by flow cytometry and confocal microscopy, and the release of cytokines from THP‐1 cells was measured by enzyme‐linked immunosorbent assay. RESULTS CPPs showed a marked enhancement of exposed PS but dramatically reduced glycoprotein Ibα expression and clustering compared with platelets stored at 4 °C. Activation of THP‐1 cells was stronger by CPPs than by platelets stored at 22 °C and 4 °C. CPP interference tests using annexin V and anti–P‐selectin showed that CPPs induced increases in PS‐ and P‐selectin–mediated phagocytosis, as well as secretion of the proinflammatory cytokine tumor necrosis factor‐α, and interleukins IL‐1β and IL‐6, but a decrease in transforming growth factor‐β production in THP‐1 cells. Surface‐exposed PS was more effective than P‐selectin for the activation of THP‐1 cells. CONCLUSION CPPs triggered PS and P‐selectin–mediated phagocytosis by macrophages and stimulated the inflammatory response of macrophages.

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“…These studies have been extensively reviewed . A modification of this technique for which thawed PLTs were resuspended in plasma rather than saline was used by the Dutch military in Bosnia and Afghanistan and adopted by other groups …”

Section: Cryopreserved Pltsmentioning

confidence: 99%

“…39 A modification of this technique for which thawed PLTs were resuspended in plasma rather than saline was used by the Dutch military in Bosnia and Afghanistan and adopted by other groups. 40,41 In vitro and ex vivo studies of cryopreserved PLTs have shown promising results. In a baboon model, 54% of thawed cryopreserved PLTs were recovered 2 hours after transfusion, more than that for liquid PLTs stored for 5 days.…”

Section: Cryopreserved Pltsmentioning

confidence: 99%

Future of platelet formulations with improved clotting profile: a short review on human safety and efficacy data

Cancelas

2019

Transfusion

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BACKGROUND Platelet (PLT) transfusion is a widely used therapy in treating or preventing bleeding and hemorrhage in patients with thrombocytopenia or trauma. Compared to the relative ease of PLT transfusion, current practice for PLT storage at room temperature (RT) for up to 5 to 7 days is inefficient, costly, wasteful, and relatively unsafe. STUDY DESIGN AND METHODS This study was a review of major advances in PLT derivative products with improved hemostatic potential and safety feature. RESULTS Recent progress in understanding the PLT activation and host clearance mechanisms has led to reassessments of current and new storage conditions that employ refrigeration and/or cryopreservation to overcome storage lesions and significantly extend shelf life of PLTs with reduced risk of pathogen contamination. DISCUSSION It is anticipated that future PLT preservation involving cold, frozen, and/or pathogen reduction strategies in proper PLT additive solutions will enable longer term and safer PLT storage.

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Vox Sanguinis International Forum on platelet cryopreservation

Cited by 23 publications

References 25 publications

Freezing expired platelets does not compromise in vitro quality: An opportunity to maximize inventory potential

Freezing expired platelets does not compromise in vitro quality: An opportunity to maximize inventory potential

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Future of platelet formulations with improved clotting profile: a short review on human safety and efficacy data

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