Voyage inside the cell: Microsystems and nanoengineering for intracellular measurement and manipulation

Li, Jun; Wen, Jialin; Zhang, Zhuoran; Liu, Haijiao; Sun, Yu

doi:10.1038/micronano.2015.20

Cited by 80 publications

(62 citation statements)

References 199 publications

(218 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1][2][3] To address this challenge,p rimary research in genetic engineering and synthetic biology focuses on understanding and engineering genetic codons to enhance cellular adaptive responses to environmental changes. [4,5] Generally,s ynthetic biology aims to develop new circuit design principles that involve multistep procedures,i ncluding creating and analyzing computational models,c onstructing genetic circuits,e valuating the performance of the genetically engineered cells,a nd refining the design process.…”

mentioning

confidence: 99%

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

et al. 2017

View full text Add to dashboard Cite

Abioactive synthetic porous shell was engineered to enable cells to survive in an oligotrophic environment. Eukaryotic cells (yeast) were firstly coated with a b-galactosidase (b-gal), before crystallization of ametal-organic framework (MOF) film on the enzyme coating;t hereby producing ab ioactive porous synthetic shell. The b-gal was an essential component of the bioactive shell as it generated nutrients (that is,glucose and galactose) required for cell viability in nutrientdeficient media (lactose-based). Additionally,the porous MOF coating carried out other vital functions,s uch as 1) shielding the cells from cytotoxic compounds and radiation, 2) protecting the non-native enzymes (b-gal in this instance) from degradation and internalization, and 3) allowing for the diffusion of molecules essential for the survival of the cells. Indeed, this bioactive porous shell enabled the survival of cells in simulated extreme oligotrophic environments for more than 7days, leading to ad ecrease in cell viability less than 30 %, versus a9 9% decrease for naked yeast. When returned to optimal growth conditions the bioactive porous exoskeleton could be removed and the cells regained full growth immediately.T he construction of bioactive coatings represents ac onceptually new and promising approach for the next-generation of cell-based researchand application, and is an alternative to synthetic biology or genetic modification.

show abstract

mentioning

confidence: 99%

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

et al. 2017

View full text Add to dashboard Cite

show abstract

“…As envisaged from the figure, clear reduction peak for the electrode is observed at pH 7. [26][27][28][29] To confirm the catalytic 105 activity we have performed the similar cyclic voltammetric studies using bare FTO coated glass substrate. The acidic and 70 basic environment may interact with the basic ZnO and acidic catechol respectively and may degenerate their activity.…”

Section: Resultsmentioning

confidence: 99%

Zinc oxide thin film based nonenzymatic electrochemical sensor for the detection of trace level catechol

et al. 2016

View full text Add to dashboard Cite

In the present work, a novel zinc oxide thin film based nonenzymatic, electrochemical sensor is developed for the detection of catechol. Zinc oxide thin film electrode on FTO conducting substrate is 5 prepared by simple and cost effective spin coating technique. The developed sensor exhibits promising cyclic voltammetric as well as amperometric response when the concentration of the catechol in phosphate buffer (pH ~7) is varied in the rage of 2 to 15 µM. The interference of the sensor for the detection of catechol is compared in presence of chlorophenol and formaldehyde. The repeatability of the sensor performance towards catechol is also investigated at different time interval. To understand the 10 underlying mechanism of catechol sensing by ZnO thin film, we have studied the phase, micro-structural and optical features of the electrode before and after electrochemical sensing experiments. It has been observed that the XRD pattern, morphology and optical transmittance of the electrode changes significantly after electrochemical interaction with catechol. Specifically, the 2D thin film morphology upon electrochemical interaction with catechol starts changing to 1D nanowire like morphology which in 15 turn influence the phase, optical transmittance as well as sensing performance. The modulation of structural, optical features and sensing performances of the developed electrode are again supported by electrochemical impedance spectroscopy.

show abstract

“…Intracellular manipulation and monitoring has been attempted using a variety of techniques . Optical methods allow high spatio‐temporal resolution and have been employed for, for example, intracellular delivery of submicron entities or for direct manipulation of subcellular components.…”

Section: Applications For Biological Samplesmentioning

confidence: 99%

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

Bunea

Glückstad

2019

Laser & Photonics Reviews

View full text Add to dashboard Cite

Optical trapping and manipulation of objects down to the Ångstrom level has revolutionized research at the smallest scales in all natural sciences. The flexibility of optical trapping methods facilitates real‐time monitoring of the dynamics of biological processes in model systems and even in living cells. Different optical trapping and manipulation approaches allow displacement of nanostructures with subnanometer precision and force measurements with femtonewton precision. Due to inherent constraints of optical methods, most optical trapping experiments are performed in water or simple aqueous solutions. However, in recent years, there is an ever‐growing interest of shifting from simple aqueous media towards more biologically‐relevant media. Precise optical trapping and manipulation, combined with state‐of‐the‐art microfabrication, will enable the development of microrobotic “surgeons” with tremendous potential for biomedical and microengineering applications. This review introduces the basics of optical trapping and discusses its applications for biological samples, with focus on trapping in biological media and strategies for overcoming the challenges of optical manipulation in complex environments as a stepping‐stone for microrobotic “surgeons.”

show abstract

Voyage inside the cell: Microsystems and nanoengineering for intracellular measurement and manipulation

Cited by 80 publications

References 199 publications

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

Zinc oxide thin film based nonenzymatic electrochemical sensor for the detection of trace level catechol

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

Contact Info

Product

Resources

About