VP2 (PTA motif) encoding DNA vaccine confers protection against lethal challenge with infectious pancreatic necrosis virus (IPNV) in trout

Ahmadivand, Sohrab; Soltani, Mehdi; Behdani, Mahdi; Evensen, Øystein; Alirahimi, Ehsan; Soltani, Elahe; Hassanzadeh, Reza; Ashrafihelan, Javad

doi:10.1016/j.molimm.2017.12.015

Cited by 40 publications

(22 citation statements)

References 37 publications

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“…High percentages of CD4-1 + , CD4-2 + , and CD8β + T lymphocytes were observed in the peripheral blood, spleen, and head kidney of fish vaccinated with pVAA compared to the tissues of fish injected with the negative control pcDNA3.1. This is in line with the findings of a previous study indicating that DNA vaccines are able to induce an adaptive cellular immune response, via the upregulation of CD4 and CD8 (60). The proliferation of antigen-specific T-cells in pVAA vaccinated flounder fish following bacterial infection indicates the induction of a specific cellular immune response and demonstrates the potential of this antigen as a vaccine candidate.…”

Section: Discussionsupporting

confidence: 91%

“…In the present study, production of specific antibodies against V. anguillarum or rVAA was detected at days 28 and 35 post-immunization. Antibody production has been previously shown to be influenced by factors such as fish size and age, water oxygenation and temperature (30,68), and antigenic dose (60). Within this study the size and age of the selected fish were approximately equal; the oxygenation, water temperature, and other rearing conditions were normalized across all tanks; and the dose of DNA plasmid was also well-controlled.…”

Section: Discussionmentioning

confidence: 91%

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A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 91%

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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“…The aim of this study was to genetically analyse IPN viruses occurring on Polish fish farms from 2001 to 2017. To achieve this, a 1,377‐bp gene fragment of the VP2 gene was sequenced and analysed, this gene having been chosen because it is important in diagnostics and also plays a role in immune protection (Ahmadivand et al., 2018; Dadar, Memari, Peyghan, & Shapouri, 2014; Salgado‐Miranda, Rojas‐Anaya, García‐Espinosa, & Loza‐Rubio, 2014). Extra sequencing of the 1,079‐bp fragment of VP5 gene responsible for viral replication confirmed the results of the phylogenetic analysis of the VP2 gene.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic characterization of Polish isolates of infectious pancreatic necrosis virus in salmonid fish

Maj‐Paluch

Matras

Borzym

et al. 2020

Journal of Fish Diseases

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Infectious pancreatic necrosis virus (IPNV) is a virus belonging to the genus Aquabirnavirus and family Birnaviridae (Abdullah, Olsen, Hodneland, & Rimstad, 2015). The virus was first isolated in North America in 1960 and later was diagnosed in Europe and Japan. Now, it seems to be a significant and the most widespread virus in global freshwater aquaculture (Alonso, Rodriguez, & Perez-Prieto, 1999). The virus has been isolated from many fish species, crustacea and molluscs (Alonso, Rodriguez Saint-Jean, & Perez-Prieto, 2003; Castric, 1997). Highly virulent strains can result in more than 90% mortality in hatchery stock, and the fish which survive an infection can remain lifelong, asymptomatic carriers of the virus. In 2016, in China virus caused mass mortality in farmed rainbow trout. Signs such as decreased appetite, mucous-like stools and darkened pigmentation were observed. In addition, marked vacuolar degeneration of the pancreatic cells with pyknotic nucleus and decreased zymogen granules, severe haemorrhage in the liver and tumidness of respiratory epithelium in gills were observed (Zhu et al., 2017). During October 2009, an outbreak of infectious pancreatic necrosis, with typical signs and high mortality, was observed in cultured rainbow trout fry in a recirculating system in Fars Province, Iran. Fish were lethargic, had poor appetite and showed erratic swimming behaviour. Dark coloration of the skin, abdominal distension, mild-to-moderate exophthalmia,

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“…5 Despite the great concerns regarding the resistant pathogen and environmental issues, the control of fish diseases has mainly focused on the antibiotics, and more recently on the vaccination. [6][7][8] Thus, there is increasing interest in using dietary immune-stimulants (such as, prebiotic, probiotic, and plant extracts) as a potential and promising alternative to RESEARCH ARTICLE improve the health status and disease control in aquaculture. 9,10 The prebiotics in fish have revealed many beneficial effects on growth performance and feed utilization, 11 intestine microbiota, and morphology, 12 and resistance against pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Could the Vitacel R200 improve the Immune Response and Hemato-Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) challenged with Aeromonas hydrophila?

Kane¹,

Al-Sharafi²,

Hamad³

2020

ijddt

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The fermentable fiber, Vitacel R200 is industrial compound cellulose exerting many beneficial health effects at the hosts. In this study, rainbow trout (Oncorhynchus mykiss) fingerling (120 ± 4.5 grams) were fed dietary 1% (10 g/kg feed) Vitacel R200 for 7 weeks, then challenged with Aeromonas hydrophila. Two weeks after the challenge, hemato-biochemical and immune parameters in the challenged fish were studied. The lysozyme and complement activity, as well as, white blood cells (WBCs), hemoglobin (Hb%), percentage of neutrophil and eosinophil in the fish fed Vitacel were significantly increased compared with the control group. Furthermore, the serum total protein, albumin, Na+, K+, P, and Ca++ concentrations in the fish fed diet containing 1% Vitacel were significantly increased, while alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST), as well as, cortisol, cholesterol, and glucose concentration were significantly decreased in comparison to the control group. These results concluded that dietary Vitacel could improve the hemato-biochemical and immune parameters, as well as, the stress response in O. mykiss infected with A. hydrophila.

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VP2 (PTA motif) encoding DNA vaccine confers protection against lethal challenge with infectious pancreatic necrosis virus (IPNV) in trout

Cited by 40 publications

References 37 publications

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Phylogenetic characterization of Polish isolates of infectious pancreatic necrosis virus in salmonid fish

Could the Vitacel R200 improve the Immune Response and Hemato-Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) challenged with Aeromonas hydrophila?

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