VP7 and VP8* genetic characterization of group A rotavirus genotype G12P[8]: Emergence and spreading in the Eastern Brazilian coast in 2014

Silva, Marcelle Figueira Marques da; Fumian, Túlio Machado; Assis, Rosane Maria Santos de; Fialho, Alexandre Madi; Carvalho-Costa, Filipe Aníbal; Andrade, Juliana da Silva Ribeiro de; Leite, José Paulo Gagliardi

doi:10.1002/jmv.24605

Cited by 25 publications

(57 citation statements)

References 51 publications

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“…Lineage III strains, on the other hand, are the mostly contemporary G12 strains detected since the mid-2000's and which are now globally prevalent in most continents. This analysis con rms that the genotype G12 strains circulating in these six sub-Saharan African countries (Ethiopia, Kenya, Rwanda, Tanzania, Togo and Zambia) clustered in lineage III with strains circulating all over the world, showing the dominance and biological competitiveness of these strains, which have persisted over the last two decades, in most continents [47][48][49]. Evidence of genetic variation was observed amongst the four G12 lineages in this study.…”

Section: Discussionsupporting

confidence: 74%

Genetic characterization of G12P[6] and G12P[8] rotavirus strains in six African countries

Rakau¹,

Nyaga²,

Gededzha³

et al. 2020

Preprint

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Background: G12 rotaviruses were first observed in sub-Saharan Africa in 2004 and since then have continued to emerge and spread across the continent and are reported as a significant human rotavirus genotype in several African countries, both prior to and after rotavirus vaccine introduction. This study investigated the genetic variability of 15 G12 rotavirus strains with either P[6] or P[8] identified between 2010 and 2014 from Ethiopia, Kenya, Rwanda, Tanzania, Togo and Zambia.Methods: The investigation was carried out by comparing VP7 and partial VP4 sequences of the African G12P[6] and G12P[8] strains with the available GenBank sequences and mapping the recognized neutralization epitopes of these strains.Results: The findings suggested that the VP7 and VP4 genes of the G12 strains circulating in African countries are homologous at the nucleotide and amino acid level, irrespective of country of origin and year of detection, although there was a unique clustering of the Ethiopian strains. The study strains shared a common ancestry with G12 strains circulating globally. Neutralization epitope mapping revealed that rotavirus VP4 P[8] genes associated with G12 had amino acids similar to those reported globally including the vaccines RotaTeq® P[8] and Rotarix®.Conclusions: It is unlikely that widespread vaccine use has driven the molecular evolution and sustainability of G12 strains in Africa. Furthermore, it is too early post vaccine introduction to indicate any effect of vaccine-induced pressure on maintaining the stability of these strains in circulation. Continuous monitoring of rotavirus genotypes is recommended to assess the long-term impact of rotavirus vaccination on the dynamic nature of rotavirus evolution on the continent.

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Section: Discussionsupporting

confidence: 74%

Genetic characterization of G12P[6] and G12P[8] rotavirus strains in six African countries

Rakau¹,

Nyaga²,

Gededzha³

et al. 2020

Preprint

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“…It is important to emphasize that lack of detection of the virus does not imply that there was no RVA circulation in this period; instead, viral detection was below the LOD, due to the low sensitivity of RT-qPCR for RVA detection. The VP4 gene was successfully characterized in all samples collected in 2015, showing the circulation of the genotypes P [4], P [8], P [9] and P [10] in Barcelona. The VP7 gene could only be genotyped in samples collected in April 2016 from Besòs and Granollers, showing the circulation of genotypes G2, G3, G9 and G12 in raw sewage from Barcelona (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…To date, there are 36 G genotypes and 51 P genotypes described in the literature (Rotavirus Classification Working Group, updated in May 2018 and available at https://rega.kuleuven.be/cev/viralmetagenomics/virus-classification/rcwg). Six G/P combinations: G1P [8], G2P [4], G3P [8], G4P [8], G9P [8] and G12P [8] are the most prevalent combinations detected in humans [3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…Rotarix ® (RV1) (GlaxoSmithKline, Rixensart, Belgium) is an attenuated monovalent vaccine, derived from a human G1P [8] strain, which is administered orally to infants at 2 and 4 months of age. Rotateq ® (RV5) (Merck and Co., Whitehouse Station, NJ, USA; Sanofi Pasteur MSD, Lyon, France) is a pentavalent vaccine comprising five human-bovine reassortant strains: four expressing human G1, G2, G3, G4 and bovine P7 [5], and one expressing P1A [8] (human) and G6 (bovine). This latter vaccine is administered orally to infants at 2, 4 and 6 months of age [12].…”

Section: Introductionmentioning

confidence: 99%

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High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain

Silva‐Sales

Martínez-Puchol

Gonzales-Gustavson

et al. 2020

Viruses

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Rotavirus A (RVA) is the most common virus associated with infantile gastroenteritis worldwide, being a public health threat, as it is excreted in large amounts in stool and can persist in the environment for extended periods. In this study, we performed the detection of RVA and human adenovirus (HAdV) by TaqMan qPCR and assessed the circulation of RVA genotypes in three wastewater treatment plants (WWTPs) between 2015 and 2016 in Catalonia, Spain. RVA was detected in 90% and HAdV in 100% of the WWTP samples, with viral loads ranging between 3.96 × 104 and 3.30 × 108 RT-PCR Units/L and 9.51 × 104 and 1.16 × 106 genomic copies/L, respectively. RVA VP7 and VP4 gene analysis revealed the circulation of G2, G3, G9, G12, P[4], P[8], P[9] and P[10]. Nucleotide sequencing (VP6 fragment) showed the circulation of I1 and I2 genotypes, commonly associated with human, bovine and porcine strains. It is important to mention that the RVA strains isolated from the WWTPs were different from those recovered from piglets and calves living in the same area of single sampling in 2016. These data highlight the importance of monitoring water matrices for RVA epidemiology and may be a useful tool to evaluate and predict possible emergence/reemergence of uncommon strains in a region.

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“…The genetic diversity of the RVA, as well as some intrinsic characteristics of these viruses, such as the temporal fluctuations of genotypes and the interspecies barrier broken to generate new combinations or novel strains will always require an active laboratory surveillance to monitor and evaluate the circulating genotypes. In this context, it is essential to maintain active surveillance studies in order to predict the possible variants that might circulate in a country, as well as to assess the effects and the impact of the RV vaccination to aid in future RVA vaccine design and development (Fumian et al, 2011;Linhares et al, 2011, da Silva et al, 20132017). In attempt to evaluate the impact of vaccination programs on viral strains circulating after RVA introduction, environmental surveillance studies were conducted in Brazil and Nicaragua (Bucardo et al, 2011, Fumian et al 2011.…”

Section: Future Aspectsmentioning

confidence: 99%