VR1-, VRL-1- and P2X3 receptor-immunoreactive innervation of the rat temporomandibular joint

Ichikawa, Hiroyuki; Fukunaga, Tomohiro; Jin, Hai Wei; Fujita, Masatoshi; Takano‐Yamamoto, Teruko; Sugimoto, Tomosada

doi:10.1016/j.brainres.2004.02.029

Cited by 54 publications

(31 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that the majority of the neurons providing innervation of the TMJ capsule co-expressed CGRP and the TRPV1 receptor. Similarly, others (Ichikawa et al, 2004;Loi et al, 2006) have reported TRPV1 and CGRP co-expression in nerves providing innervation of cells lining the synovium of the TMJ capsule. TRPV1 receptors, which are activated by capsaicin, heat, and protons, are abundantly expressed by unmyelinated C-fibers involved in pain transmission (Caterina et al, 1997).…”

Section: Discussionmentioning

confidence: 70%

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Abbey

Patil

Vause

et al. 2008

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Ethnopharmacological relevance-Cocoa bean preparations were first used by the ancient Maya and Aztec civilizations of South America to treat a variety of medical ailments involving the cardiovascular, gastrointestinal, and nervous systems. Diets rich in foods containing abundant polyphenols, as found in cocoa, underlie the protective effects reported in chronic inflammatory diseases. Release of calcitonin gene-related peptide (CGRP) from trigeminal nerves promotes inflammation in peripheral tissues and nociception.Aim of the study-To determine whether a methanol extract of Theobroma cacao L. (Sterculiaceae) beans enriched for polyphenols could inhibit CGRP expression, both an in vitro and an in vivo approach was taken.Results-Treatment of rat trigeminal ganglia cultures with depolarizing stimuli caused a significant increase in CGRP release that was repressed by pretreatment with Theobroma cacao extract. Pretreatment with Theobroma cacao was also shown to block the KCl-and capsaicin-stimulated increases in intracellular calcium. Next, the effects of Theobroma cacao on CGRP levels were determined using an in vivo model of temporomandibular joint (TMJ) inflammation. Capsaicin injection into the TMJ capsule caused an ipsilateral decrease in CGRP levels. Theobroma cacao extract injected into the TMJ capsule 24 h prior to capsaicin treatment repressed the stimulatory effects of capsaicin.Conclusions-Our results demonstrate that Theobroma cacao extract can repress stimulated CGRP release by a mechanism that likely involves blockage of calcium channel activity. Furthermore, our findings suggest that the beneficial effects of diets rich in cocoa may include suppression of sensory trigeminal nerve activation.

show abstract

Section: Discussionmentioning

confidence: 70%

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Abbey

Patil

Vause

et al. 2008

Journal of Ethnopharmacology

View full text Add to dashboard Cite

show abstract

“…The basis for the selective enhancement of E2 on ATP-evoked activity of NS units in laminae I-II is not certain. Six of the seven P2X receptor subtypes are expressed in trigeminal ganglion cells (Ambalavanar et al 2005;Collo et al 1996) and nearly 50% of TMJ afferents express P2X3 receptors (Ichikawa et al 2004;Shinoda et al 2005) and 30% of TMJ sensory neurons are isolectin B4 (IB4) positive (Flake et al 2004). Small-diameter sensory fibers that express IB4 project only to laminae I-II in Vc (Ambalavanar and Morris 1993; Kobayashi and Matsumura 1996) and may be the target of E2 modulation.…”

Section: Estrogen Status and Atp-evoked Articular Input To Tmj Unitsmentioning

confidence: 99%

“…Studies in rat and cat indicate the TMJ region is innervated mainly by small-diameter myelinated and unmyelinated fibers (Ioi et al 2006;Kido et al 1995;Takeuchi and Toda 2003;Takeuchi et al 2001) that project to second-order neurons at the trigeminal subnucleus caudalis/upper cervical cord (Vc/C1-2) junction (Shigenaga et al 1986(Shigenaga et al , 1988. Lesion of the Vc/C1-2 junction, but not more rostral trigeminal brain stem areas, prevents increases in masseter muscle activity after TMJ inflammation (Hu et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…In animal studies injection of physiological concentrations of ATP or its analogues into the knee joint activates nociceptive afferent fibers (Dowd et al 1998), whereas injection into the TMJ produces nocifensive behavior in rats (Oliveira et al 2005). ATP excites nociceptors by acting mainly on P2X2 and P2X3 receptors and a significant percentage of trigeminal ganglion cells that innervate the TMJ (Ichikawa et al 2004;Shinoda et al 2005), masseter muscle (Ambalavanar et al 2005;Connor et al 2005), or tooth pulp (Cook et al 1997) express these receptor subtypes. In the trigeminal brain stem, P2X receptor blockade inhibits the sensitization of rostral Vc and trigeminal subnucleus oralis neurons caused by acute tooth pulp nerve injury (Chiang et al 2005;Hu et al 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential Effects of Estradiol on Encoding Properties of TMJ Units in Laminae I and V at the Spinomedullary Junction in Female Rats

Tashiro¹,

Okamoto²,

Milam³

et al. 2007

Journal of Neurophysiology

View full text Add to dashboard Cite

Tashiro A, Okamoto K, Milam SB, Bereiter DA. Differential effects of estradiol on encoding properties of TMJ units in laminae I and V at the spinomedullary junction in female rats. J Neurophysiol 98: 3242-3253, 2007. First published October 10, 2007 doi:10.1152/jn.00677.2007. To determine whether estrogen status modulated dorsal horn neural activity relevant to temporomandibular joint (TMJ) processing single units were recorded in superficial and deep laminae at the trigeminal subnucleus caudalis/upper cervical cord (Vc/C1-2) junction of ovariectomized (OvX) female rats under barbiturate anesthesia after 17␤-estradiol (E2) treatment for 2 days. E2 dose-dependently enhanced the response to intra-TMJ stimulation by adenosine triphosphate (ATP) of neurons classified as nociceptive specific (NS), but not wide dynamic range (WDR), in superficial laminae. ATP caused similar responses among NS and WDR neurons from deep laminae in all groups. By contrast, the cutaneous receptive field areas of WDR, but not NS, units in superficial and deep laminae were enlarged in high E2-treated (HE2) compared with low E2-treated (LE2) females. Units from untreated or vehicle-treated male rats displayed responses similar to those of LE2 females. TMJ units in superficial laminae from females were more likely to receive convergent cutaneous input and respond to jaw movement than males, independent of E2 treatment. Western blot analysis revealed similar levels of P2X2 and P2X3 receptor protein in Vc/C1-2 or trigeminal ganglion samples in all groups. Immunohistochemistry revealed dense terminal labeling for P2X3 receptors in superficial laminae and moderate labeling in deep laminae at the Vc/C1-2 junction. These data indicated a significant linkage between estrogen status and the magnitude of articular input evoked by ATP from TMJ neurons in the superficial laminae at the Vc/C1-2 junction, whereas estrogenic modulation of TMJ neurons in deep laminae affected only the convergent input from overlying facial skin.

show abstract

“…Many previous studies showed the expression of P2X3 in the central and peripheral neurons 1,4,[8][9][10] . In the orofacial region, it has been reported that P2X3 immunoreactivity exists in dental pulp 3) , taste bud 8) , and temporomandibular joint 11) of the rat. The presence of P2X3 in the human dental pulp was also documented in some studies 12,13) .…”

Section: ⅰ Introductionmentioning

confidence: 99%

Expression of P2X₃and its colocalization with trpv1 in the human dental pulp

Kim

2007

J Korean Acad Conserv Dent

View full text Add to dashboard Cite

The purinoreceptor, P2X3 is a ligand-gated cation channel activated by extracellular ATP. It has been reported that ATP can be released during inflammation and tissue damage, which in turn may activate P2X3 receptors to initiate nociceptive signals. However, little is known about the contribution of P2X3 to the dental pain during pulpal inflammation. Therefore, the purpose of this study was to investigate the expression of P2X3 and its colocalization with TRPV1 to understand the mechanism of pain transmission through P2X3 in the human dental pulp with double labeling immunofluorescence method.In the human dental pulp, intense P2X3 immunoreactivity was observed throughout the coronal and radicular pulp. Of all P2X3-positive fibers examined, 79.4% coexpressed TRPV1.This result suggests that P2X3 along with TRPV1 may be involved in the transmission of pain and potentiation of noxious stimuli during pulpal inflammation. [J Kor Acad Cons Dent 32(6):514-521, 2007]

show abstract

VR1-, VRL-1- and P2X3 receptor-immunoreactive innervation of the rat temporomandibular joint

Cited by 54 publications

References 19 publications

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Differential Effects of Estradiol on Encoding Properties of TMJ Units in Laminae I and V at the Spinomedullary Junction in Female Rats

Expression of P2X₃and its colocalization with trpv1 in the human dental pulp

Contact Info

Product

Resources

About

VR1-, VRL-1- and P2X3 receptor-immunoreactive innervation of the rat temporomandibular joint

Cited by 54 publications

References 19 publications

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Differential Effects of Estradiol on Encoding Properties of TMJ Units in Laminae I and V at the Spinomedullary Junction in Female Rats

Expression of P2X3and its colocalization with trpv1 in the human dental pulp

Contact Info

Product

Resources

About

Expression of P2X₃and its colocalization with trpv1 in the human dental pulp