VX2-induced rabbit buccal carcinoma: A potential cancer model for human buccal mucosa squamous cell carcinoma

Lin, Li‐Min; Chen, Yuk-Kwan; Chen, Chi‐Hsien; Chen, Yu‐Wen; Huang, Anderson Hsien-Cheng; Wang, Wen-Chen

doi:10.1016/j.oraloncology.2009.07.012

Cited by 14 publications

(8 citation statements)

References 42 publications

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“…The common sites of tumor implantation in this model include the leg muscle, liver, and auricles [15] , [16] , [17] . Studies have also established VX2 tumors in the buccal mucosa, larynx, and esophagus [18] , [19] , [20] . To mimic thick neck masses seen in humans, we developed an orthotopic neck tumor model by surgically implanting tumor tissue into the sternohyoid muscle of rabbits.…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal Optoacoustic Mapping of Tumor Hemodynamics in a Clinically Relevant Orthotopic Rabbit Model of Head and Neck Cancer

Rich

Sexton

Curtin

et al. 2017

Translational Oncology

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The purpose of this study was to investigate the usefulness of photoacoustic imaging (PAI) for spatiotemporal mapping of tumor hemodynamics in a rabbit model of head and neck carcinoma. Shope cottontail rabbit papilloma virus associated VX2 carcinomas were established in adult male New Zealand White rabbits (n = 9) by surgical transplantation of tumor tissue in the neck. Noninvasive PAI with co-registered ultrasound (US) was performed to longitudinally monitor tumor growth, oxygen saturation (%sO2), and hemoglobin concentration (HbT). PAI findings were validated with Doppler sonography measures of percent vascularity (PV). Differences in tumor volumes, %sO2, HbT, and PV values over time were analyzed using repeated-measures analysis of variance with multiple comparisons. Two-tailed Spearman correlation analysis was performed to determine the correlation coefficient (r) for comparisons between %sO2, HbT, and tumor volume. US revealed a significant (P < .0001) increase in tumor volume over the 3-week period from 549 ± 260 mm3 on day 7 to 5055 ± 438 mm3 at 21 days postimplantation. Consistent with this aggressive tumor growth, PAI revealed a significant (P < .05) and progressive reduction in %sO2 from day 7 (37.6 ± 7.4%) to day 21 (9.5 ± 2.1%). Corresponding Doppler images also showed a decrease in PV over time. PAI revealed considerable intratumoral spatial heterogeneity with the tumor rim showing two- to three-fold higher %sO2 values compared to the core. Noninvasive PAI based on endogenous contrast provides a label-free method for longitudinal monitoring of temporal changes and spatial heterogeneity in thick head and neck tumors.

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Section: Discussionmentioning

confidence: 99%

Spatiotemporal Optoacoustic Mapping of Tumor Hemodynamics in a Clinically Relevant Orthotopic Rabbit Model of Head and Neck Cancer

Rich

Sexton

Curtin

et al. 2017

Translational Oncology

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“…All animal experiments were completed following a protocol approved by the Animal Care Committee of University Health Network, Toronto, Ontario, Canada, with measures to minimize pain and discomfort. To generate the head and neck tumor model, ten male New Zealand white rabbits (Charles River, Wilmington, Massachusetts) weighing 2.5-3.0 kg were anesthetized with isoflurane and subsequently injected with 300 μL of a high-density cell suspension (5 x 10 6 /mL) of VX2 squamous cell carcinoma into one cheek muscle (buccinator) [14]. Two weeks after VX2 cell injection all ten animals developed tumor in the cheek and cervical lymph node metastases.…”

Section: Animal Modelmentioning

confidence: 99%

Sentinel lymph node mapping using ICG fluorescence or cone beam CT - a rabbit model feasibility study

Muhanna

Chan

Douglas

et al. 2020

Preprint

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Background Current sentinel lymph node biopsy (SLNB) techniques, including use of radioisotopes, have disadvantages including the use of a radioactive tracer. Indocyanine green (ICG) based near-infrared (NIR) fluorescence imaging and cone beam CT (CBCT) have advantages for intraoperative use. However, limited literature exists regarding their use in head and neck cancer SLNB.Methods This was a prospective, non-randomized study using a rabbit oral cavity VX2 squamous cell carcinoma model which develops lymph node metastasis. Pre-operatively, images were acquired by MicroCT and DCE-MRI. CBCT and NIR fluorescence imaging of ICG was used to map and guide the SLNB resection.Results Intraoperative use of ICG to guide fluorescence resection resulted in identification of all lymph nodes identified by pre-operative CT. CBCT was useful for near real time intraoperative imaging and 3D reconstruction.Conclusions This pre-clinical study further demonstrates the technical feasibility, limitations and advantages of intraoperative NIR-guided ICG imaging for SLN identification as a complementary method during head and neck surgery.

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“…To generate the head and neck tumor model, ten male New Zealand white rabbits (Charles River, Wilmington, Massachusetts) weighing 2.5-3.0 kg were anesthetized with iso urane and subsequently injected with 300 μL of a high-density cell suspension (5 x 10 6 /mL) of VX2 squamous cell carcinoma into one cheek muscle (buccinator) [18]. Two weeks after VX2 cell injection all ten animals developed tumor in the cheek and cervical lymph node metastases.…”

Section: Animal Modelmentioning

confidence: 99%

Sentinel lymph node mapping using ICG fluorescence and cone beam CT – a feasibility study in a rabbit model of oral cancer

Muhanna

Chan

Douglas

et al. 2020

Preprint

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Background: Current sentinel lymph node biopsy (SLNB) techniques, including use of radioisotopes, have disadvantages including the use of a radioactive tracer. Indocyanine green (ICG) based near-infrared (NIR) fluorescence imaging and cone beam CT (CBCT) have advantages for intraoperative use. However, limited literature exists regarding their use in head and neck cancer SLNB.Methods: This was a prospective, non-randomized study using a rabbit oral cavity VX2 squamous cell carcinoma model (n = 10) which develops lymph node metastasis. Pre-operatively, images were acquired by MicroCT. During surgery, CBCT and NIR fluorescence imaging of ICG was used to map and guide the SLNB resection.Results: Intraoperative use of ICG to guide fluorescence resection resulted in identification of all lymph nodes identified by pre-operative CT. CBCT was useful for near real time intraoperative imaging and 3D reconstruction. Conclusions: This pre-clinical study further demonstrates the technical feasibility, limitations and advantages of intraoperative NIR-guided ICG imaging for SLN identification as a complementary method during head and neck surgery.

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VX2-induced rabbit buccal carcinoma: A potential cancer model for human buccal mucosa squamous cell carcinoma

Cited by 14 publications

References 42 publications

Spatiotemporal Optoacoustic Mapping of Tumor Hemodynamics in a Clinically Relevant Orthotopic Rabbit Model of Head and Neck Cancer

Spatiotemporal Optoacoustic Mapping of Tumor Hemodynamics in a Clinically Relevant Orthotopic Rabbit Model of Head and Neck Cancer

Sentinel lymph node mapping using ICG fluorescence or cone beam CT - a rabbit model feasibility study

Sentinel lymph node mapping using ICG fluorescence and cone beam CT – a feasibility study in a rabbit model of oral cancer

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