W-band Pulsed ENDOR of Transition Metal Centers in Orientationally Disordered Systems and Single Crystals

Goldfarb, Daniella; Krymov, Vladimir

doi:10.1007/978-1-4757-4379-1_10

Cited by 6 publications

(2 citation statements)

References 71 publications

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“…Further on, the first HF pulsed EPR spectrometer was built in Leiden at 95 GHz (Weber et al 1989). Since 1996, a 95 GHz pulsed HF-EPR spectrometer has been offered commercially by Bruker Analytik (Höfer et al 1996), as well as by Donetsk Physico Technical Institute (Goldfarb and Krymov 2004) at 95 and 140 GHz microwave frequencies. Numerous recent review articles exist, which discuss the main technical issues of cw and pulsed HF-EPR spectrometers (Prisner 1997, Freed 2000, Smith and Riedi 2000.…”

Section: Instrumentationmentioning

confidence: 99%

New developments in high field electron paramagnetic resonance with applications in structural biology

Bennati

Prisner²

2005

Rep. Prog. Phys.

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Recent developments in microwave technologies have led to a renaissance of electron paramagnetic resonance (EPR) due to the implementation of new spectrometers operating at frequencies 90 GHz. EPR at high fields and high frequencies (HF-EPR) has been established up to THz (very high frequency (VHF) EPR) in continuous wave (cw) operation and up to about 300 GHz in pulsed operation. To date, its most prominent application field is structural biology. This review article first gives an overview of the theoretical basics and the technical aspects of HF-EPR methodologies, such as cw and pulsed HF-EPR, as well as electron nuclear double resonance at high fields (HF-ENDOR). In the second part, the article illustrates different application areas of HF-EPR in studies of protein structure and function. In particular, HF-EPR has delivered essential contributions to disentangling complex spectra of radical cofactors or reaction intermediates in photosynthetic reaction centres, radical enzymes (such as ribonucleotide reductase) and in metalloproteins. Furthermore, HF-EPR combined with site-directed spin labelling in membranes and soluble proteins provides new methods of investigating complex molecular dynamics and intermolecular distances.

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Section: Instrumentationmentioning

confidence: 99%

New developments in high field electron paramagnetic resonance with applications in structural biology

Bennati

Prisner²

2005

Rep. Prog. Phys.

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“…Furthermore, at high magnetic fields it is often possible to resolve all components of the anisotropic g-tensor. This orientation selectivity allows the determination of the orientation of the hyperfine tensor with respect to the g-tensor axis system even in disordered powder samples [8][9][10]. Unfortunately this advantage does not hold for methods like ESEEM or HYSCORE, since they rely on forbidden transitions whose transition moments are considerably attenuated at high magnetic fields [11].…”

Section: Introductionmentioning

confidence: 99%

2D-REFINE spectroscopy: Separation of overlapping hyperfine spectra

Cernescu

Maly

Prisner

2008

Journal of Magnetic Resonance

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High-field/High-frequency EPR Spectroscopy in Protein Research: Principles and Examples

Möbius

Savitsky

2022

Appl Magn Reson

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During the last decades, the combined efforts of biologists, chemists, and physicists in developing high-field/high-frequency EPR techniques and applying them to functional proteins have demonstrated that this type of magnetic resonance spectroscopy is particularly powerful for characterizing the structure and dynamics of stable and transient states of proteins in action on biologically relevant time scales ranging from nanoseconds to hours. The review article describes how high-field EPR methodology, in conjunction with site-specific isotope and spin-labeling strategies, is capable of providing new insights into fundamental biological processes. Specifically, we discuss the theoretical and instrumental background of continuous-wave and pulse high-field EPR and the multiple-resonance extensions EDNMR, ENDOR, TRIPLE, ESEEM, PELDOR, and RIDME. Some emphasis is placed on a balanced description of both the historical spadework and the achieved performance of advanced EPR at 95 GHz and 360 GHz. This culminates in a coherent treatment of state-of-the-art research of high-field EPR in terms of both instrumentation development and application to representative protein complexes such as cofactor binding sites in photosynthesis.

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W-band Pulsed ENDOR of Transition Metal Centers in Orientationally Disordered Systems and Single Crystals

Cited by 6 publications

References 71 publications

New developments in high field electron paramagnetic resonance with applications in structural biology

New developments in high field electron paramagnetic resonance with applications in structural biology

2D-REFINE spectroscopy: Separation of overlapping hyperfine spectra

High-field/High-frequency EPR Spectroscopy in Protein Research: Principles and Examples

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