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Abstract

Several antimicrobial agents have already been investigated relating to their influence on neutrophil ROS generation. Azithromycin provides, a dose-related anti-oxidant effect, after 15 min incubation, with the stimulating agent FMLP, as well with PMA or S. aureus. This finding was however obtained with concentrations not considered in therapeutics. Since short incubation times are not representative of the physiological situation, and since azithromycin is characterized by prolonged high concentrations within phagocytes, the same experiments were performed over 2 and 4 h exposures. A time-dependent anti-oxidant effect was then reported. The maximum effect was obtained with PMA (IC50 were 856 and 30 micrograms/ml for 15 min and 4 h incubation times respectively). Time-dependent modifications of neutrophil oxidative metabolism seem to be correlated with intracellular concentrations. Depressed oxidative metabolism might be related neither to azithromycin cellular toxicity, nor to superoxide scavenging properties. By increasing exposure periods, therapeutic concentrations could therefore lead to an anti-inflammatory effect, potentially of clinical interest since associated with bacteriostatic activity.

Effects of Various Drugs on Alcohol-induced Oxidative Stress in the Liver

“…Azithromycin is an antibiotic with a wide range of imunomodulatory effects [ 19 , 20 , 21 ] used in the treatment of number of bacterial infections, due to inhibition of the generation of.…”

“…Levert et al . [ 19 ] show that azithromycin inhibits the generation of O 2 in the presence of agents which stimulate neutrophile oxidative burst. Consequently, azithromycin exhibits a strong anti-inflammatory and antioxidative effect with very low IC50 values.…”

Neurosedative and Antioxidant Activities of Phenylpropanoids from Ballota nigra

“…All results expressed in IC 50 were calculated with a computer program [37]. Results were expressed as mean values ± standard deviation (SD) and were compared using the Wilcoxon test as previously described [38]. Differences were considered to be statistically significant when p < 0.05.…”

Azithromycin attenuates cigarette smoke extract-induced oxidative stress injury in human alveolar epithelial cells

“…The present study demonstrated, for the first time to the best of our knowledge, that AZM decreases ROS generation induced by CSE. AZM, a macrolide antibiotic widely used in clinical practice, was reported to suppress neutrophil ROS release ( 27 ). In a cystic fibrosis airway epithelial cell line, AZM significantly reduced the activity of glutathione transferase ( 28 ).…”

“…It accumulates at a high rate in cells and tissues and has a plasma half-life of >40 h ( 26 ). In the airway epithelial cells and neutrophils of cystic fibrosis patients, AZM has been demonstrated to have antioxidant capacity ( 27 – 29 ). However, there have been no studies, to the best of our knowledge, investigating the possible role of AZM in the protection of human AECs from oxidative injury induced by CSE.…”