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Saeed, Nasir; Zafar, Yusuf; Malik, K. A.

doi:10.1023/a:1005958812583

Cited by 27 publications

(4 citation statements)

References 10 publications

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“…Cell competency depends on hormones inside the callus tissue [21]. This result is in accordance with the result of Saeed, et al [22]. He states that the multiplication of shoots derived from direct shoot organogenesis occurred in a shorter period than shoots formed in the callus phase.…”

Section: Figure 4 Shoots Derived From Callus (D E G H I) and Shoosupporting

confidence: 86%

In Vitro Regeneration of Foxtail Millet (Setaria italica (L.) Beauv.) cv. Buru Hotong

Iriawati

Puspita

Rodiansyah

2017

J. Math. Fund. Sci.

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Abstract. In vitro regeneration of foxtail millet (Setaria italica (L.) Beauv.) was done using basal shoot explants of 10-day old seedlings. Explants were cultured in MS basal medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP) and 1.5 ppm NiSO 4 . Shoot multiplication and root induction were done in Murashige and Skoog (MS) basal media. Plantlets were then acclimatized in rice husk charcoal, cocopeat, or mixed media. Results showed that MS basal medium containing 0.5 ppm kinetin, 2 ppm BAP, and 0.1 ppm 2,4-D was the optimal medium for shoot induction, in which 60% of explants developed direct shoot organogenesis. In addition, callus was optimally formed in MS medium supplemented by 1 ppm kinetin, 1 ppm BAP, and 0.5 ppm 2,4-D. Regenerated shoots spontaneously developed roots after being transferred into MS basal media without growth regulator. During acclimatization, the highest survival rate of plantlets (47%) was obtained in rice husk charcoal. The developed method could be useful towards improvement of this species using in vitro tissue culture techniques.

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Section: Figure 4 Shoots Derived From Callus (D E G H I) and Shoosupporting

confidence: 86%

In Vitro Regeneration of Foxtail Millet (Setaria italica (L.) Beauv.) cv. Buru Hotong

Iriawati

Puspita

Rodiansyah

2017

J. Math. Fund. Sci.

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“…Cotton has successfully been regenerated from the shoot apex. Problem related to regeneration of plant from callus has been sorted out by using shoot apex as explant [21][22][23]. Cotton was regenerated by the use of shoot apex which was less expensive and required less labor to regenerate cotton plant.…”

Section: Cell and Tissue Culture Technologymentioning

confidence: 99%

Introductory Chapter: Recent Trends in “Cotton Research”

Zaib¹,

Iqbal²,

Shahzadi³

et al. 2020

Advances in Cotton Research

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“…Gould et al (1991) extended this approach by using two G. barbadense cultivars and 19 G. hirsutum cultivars in his research, which showed that regeneration from shoot tips was genotype-independent. Saeed et al (1997), Morre et al (1998) and Zapata et al (1999) also reported the regeneration of cotton plants from shoot meristems. However, rooting efficiencies were low in these reports (from 38 to 58%).…”

Section: Introductionmentioning

confidence: 96%

Optimization of micropropagation protocol for three cotton varieties regenerated from apical shoot

Afolabi-Balogun¹,

Inuwa²,

Ume³

et al. 2015

J. Plant Breed. Crop Sci.

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The need for alternative strategies to obtain transgenic cotton via apical shoot was necessitated due to the recalcitrance of cotton regeneration from somatic embryogenesis, this has greatly slowed down the development of transgenic cottons. To this effect, an optimized regeneration system from apical shoot was developed for three varieties of cotton. Ninety-five percent seed surface sterility was observed in seed germination using a combination of hydrogen perioxide and Clorox as sterilizing medium. Highest shoot elongation rate was achieved on MS supplemented with 2.5 mg/L BAP + 0.1% (w/v) AC, rapid shoot growth occurred with kinetin supplemented media. Rooting efficiency of the three improved cultivars of cotton (Gossypium hirsutum), Samcot 9,11 and 13 were optimized using the optimum medium for rooting of difficult-to-root in vitro regenerated shoots of cotton which consist of MS basal salts and modified MS vitamins, supplemented with 3% sucrose, 0.2 mg/L IBA, without activated charcoal. In the end, an improved regeneration protocol with rooting efficiency up to 47% and regeneration rate up to 87% by combining rooting induction, indole acetic acid (IAA) shock and graft technique was developed.

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Untitled

Cited by 27 publications

References 10 publications

In Vitro Regeneration of Foxtail Millet (Setaria italica (L.) Beauv.) cv. Buru Hotong

In Vitro Regeneration of Foxtail Millet (Setaria italica (L.) Beauv.) cv. Buru Hotong

Introductory Chapter: Recent Trends in “Cotton Research”

Optimization of micropropagation protocol for three cotton varieties regenerated from apical shoot

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