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Abstract

Jasminum polyanthum is an important ornamental plant belonging to the Oleaceae family. Both shoot tip and leaves were used as explant for the induction of shoots via indirect organogenesis. For callus induction, explants were excised from field grown mature plants and thereafter incubated on MS basal medium supplied by 1.0 mg/l BA supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.0, 1.0, 2.0, 4.0 and 6.0 mg/l. Maximum percentage of callus induction from both explants was procured by the application of 2,4-D at 2.0 mg/l. Significant release of callus induction was swelled from leaf explants when compared to shoot tips at different 2,4-D levels. For the proliferation stage, induced callus was transferred into MS supplemented with BA at 0.0, 1.0, 2.0 and 3.0 mg/l along with NAA at 0.0, 0.5 and 1.0 mg/l. The maximum percentage of shoot proliferation per culture was achieved from callus cultured in 1.0 mg/L BA along with 0.5 mg/l NAA. For rooting, microshoots were treated with either IBA or IAA at 0.0, 0.5, 1.0 and 2.0 mg/l. Maximum rooting formation (Number and length) was recorded by the application of IBA at 2.0 mg/l. For acclimatization, the complete plantlets grown in pots filled with peat moss + vermiculite was suitable for 91% plantlets to survive.