2001
DOI: 10.1023/a:1013316816955
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Cited by 29 publications
(4 citation statements)
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“…The PCR product was first cloned into pBluescript SK(Ϫ) and confirmed by sequencing. Then the CBF3 ORF was released by digesting with XbaI and KpnI, and subcloned into pBIB vector under control of the superpromoter, which consists of three copies of the octopine synthase upstream-activating sequence in front of the manopine synthase promoter (28). Agrobacterium tumefaciens strain GV3101 containing this binary construct was used to transform los4-1 mutant plants.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The PCR product was first cloned into pBluescript SK(Ϫ) and confirmed by sequencing. Then the CBF3 ORF was released by digesting with XbaI and KpnI, and subcloned into pBIB vector under control of the superpromoter, which consists of three copies of the octopine synthase upstream-activating sequence in front of the manopine synthase promoter (28). Agrobacterium tumefaciens strain GV3101 containing this binary construct was used to transform los4-1 mutant plants.…”
Section: Methodsmentioning
confidence: 99%
“…We hypothesized that the defect in the regulation of CBF genes in los4-1 may be responsible for the chilling and freezing sensitivity of the mutant plants. To test this hypothesis, CBF3 was expressed under a strong constitutive super promoter (28) in los4-1 mutant plants (Fig. 4).…”
Section: Ectopic Expression Of Cbf3 In Los4-1 Suppresses the Chillingmentioning
confidence: 99%
“…The TaRAV1 transgenic lines were generated by cloning the coding sequence of TaRAV1 into the pCAMBIA1300 vector and transferred into wild-type Arabidopsis plants (Columbia ecotype) [ 58 ]. Wide-type and transgenic Arabidopsis seeds were sterilized with 1% NaClO solution for 10 min, followed by washing with sterile water.…”
Section: Methodsmentioning
confidence: 99%
“…The 35S::GhKT2 construct was generated by cloning the coding sequence of the GhKT2 gene into the binary vector, pBI121 under the control of the CaMV 35S promoter. The SUPER::GhKT2 construct was generated by cloning the coding sequence of GhKT2 into the pBI121 vector under control of the SUPER promoter [33 ] .…”
Section: Construction Of 35s::ghkt2mentioning
confidence: 99%