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Doğan, Zehra; Atli, Gülüzar; Canli, Mustafa

doi:10.4194/1303-2712-v15_2_04

Cited by 7 publications

(5 citation statements)

References 51 publications

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“…However, the downregulation of Na + /K + -ATPase and AQP3 observed here more likely suggests a stereotypical mechanical defense function to reduce the intake of Pb than an attempt to improve osmotic exchanges. The responses of Na + /K + -ATPase were not consistent in different fish species when faced with Pb exposure, and in the gills, a decreased [ 29 , 61 , 64 ] or increased activity [ 65 , 66 ] have been documented. On the issue of AQP3, we are not aware of other reports concerning the modulation after exposure to Pb in freshwater species; still, the results presented here agree with our previous results on a seawater species [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Morphological and Functional Alterations Induced by Two Ecologically Relevant Concentrations of Lead on Danio rerio Gills

Curcio

Macirella

Sesti

et al. 2022

IJMS

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Lead (Pb), due to its high toxicity and bioaccumulation tendency, is one of the top three pollutants of concern for both humans and wildlife and occupies second place in the Priority List of Hazardous Substances. In freshwater fish, Pb is mainly absorbed through the gills, where the greatest accumulation occurs. Despite the crucial role of gills in several physiological functions such as gas exchange, water balance, and osmoregulation, no studies evaluated the effects of environmentally relevant concentrations of Pb on this organ, and existing literature only refers to high levels of exposure. Herein we investigated for the first time the molecular and morphological effects induced by two low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) on the gills of Danio rerio, a model species with a high translational value for human toxicity. It was demonstrated that Pb administration at even low doses induces osmoregulatory dysfunctions by affecting Na+/K+-ATPase and AQP3 expression. It was also shown that Pb upregulates MTs as a protective response to prevent cell damage. Modulation of SOD confirms that the production of reactive oxygen species is an important toxicity mechanism of Pb. Histological and morphometric analysis revealed conspicuous pathological changes, both dose- and time-dependent.

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Section: Discussionmentioning

confidence: 99%

Morphological and Functional Alterations Induced by Two Ecologically Relevant Concentrations of Lead on Danio rerio Gills

Curcio

Macirella

Sesti

et al. 2022

IJMS

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“…Homogenizing buffers were prepared as described in the published literature: GST (Frasco & Guilhermino, 2002 ), AChE (Ellman et al, 1961 ), and (Na + ,K + )‐ATPase (Mayer‐Gostan & Lemaire, 1991 ). Homogenized shrimps were then centrifuged at 4 °C (Sigma; 3‐16KL centrifuge) for 30 min at 9000 g for GST and AChE, whereas a speed of 13 000 g was used for (Na + ,K + )‐ATPase, as described in Dogan et al ( 2015 ), modified from Mayer‐Gostan and Lemaire ( 1991 ). The supernatants were then used to assess enzyme activity for each of the three biochemical assays.…”

Section: Methodsmentioning

confidence: 99%

“…Activity of (Na + ,K + )‐ATPase was assessed for shrimp exposed to Ba 2+ and NaCl and measured using the method described by Mayer‐Gostan and Lemaire ( 1991 ) with modifications to centrifugation speeds as described in Dogan et al ( 2015 ). Centrifuged supernatant (10 µl) was diluted 10‐fold in 4 °C buffered reaction and vortexed, then 10 µl of 10‐fold diluted supernatant was added to each of the triplicate wells.…”

Section: Methodsmentioning

confidence: 99%

Mixture Toxicity of Three Unconventional Gas Fracking Chemicals, Barium, O‐Cresol, and Sodium Chloride, to the Freshwater Shrimp Paratya australiensis

Willems

Kumar

Nugegoda

2023

Enviro Toxic and Chemistry

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The 96-h acute toxicity of barium (Ba 2+ ), o-cresol, and sodium chloride (NaCl) to Paratya australiensis was assessed in single, binary, and ternary combinations in addition to three biochemical assays: glutathione S-transferase, acetylcholinesterase, and sodium-potassium adenosine triphosphatase. The 96-h lethal concentrations that expressed 50% mortality (LC50) in the single-toxicant exposures were Ba 2+ = 23.4 mg/L, o-cresol = 12.2 mg/L, and NaCl = 4198 mg/L. Mortality from ocresol exposure occurred between 11 and 22 mg/L, whereas Ba 2+ was more gradual across 10-105 mg/L, and most of the NaCl mortality occurred between 2050 and 4100 mg/L. Toxic units were used to assess the binary and ternary interactions of the toxicants. A more than additive effect was observed for most combinations in the binary chemical exposures, with the ternary combinations yielding highly synergistic interactions. Greater synergism was observed with the 96-h LC50 of o-cresol in combination with the three concentrations of NaCl (1025, 2050, and 3075 mg/L) compared with Ba 2+ , with toxic units of 0.38, 0.48, and 0.10 (o-cresol) and 0.71, 0.67, and 0.50 (Ba 2+ ). No notable enzyme activity trends were observed in the enzyme biomarker responses from both individual and mixture exposures. Although acute single-species toxicity tests tend to underestimate the effects of Ba 2+ , o-cresol, and NaCl on populations, communities, and ecosystems in seminatural (e.g., mesocosms) and natural systems, there are currently no published acute toxicity data available for P. australiensis and the three toxicants used in the present study. The present study shows that chemicals with different toxicity mechanisms can potentially lead to more synergistic responses.

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“…Data presented its activity change as mostly in decreases could be in relation to disruption of ion concentrations after Hg 2+ and Ni 2+ exposures at different Ca 2+ environment. This decrease could be also associated with metal binding directly to the enzyme structure leading to a dysfunction in its activity (Dogan, Atli, & Canli, 2015). However different pattern was observed in Mg 2+ -ATPase activity as both increases and decreases in relation to metal and Ca 2+ concentration differences.…”

Section: Atpase Activitiesmentioning

confidence: 99%

Untitled

Atli¹

2019

Turk. J. Fish. Aquat. Sci.

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In this study, freshwater fish Oreochromis niloticus were exposed to acute (3 days) and chronic (30 days) Hg 2+ and Ni 2+ in differing Ca 2+ levels (15, 30 and 90 mg Ca 2+ /L). In the acute duration 30 µM Ni 2+ and 0.3 μM Hg 2+ , in the chronic duration 10 µM Ni 2+ and 0.03 μM Hg 2+ were tested. At the end of the exposures, activities of ATPase (Total-ATPase, Na + /K +-ATPase and Mg 2+-ATPase) and AChE were measured in the brain. There were changes upon Hg 2+ and Ni 2+ effects depending on the Ca 2+ concentration though low dependence on Ca 2+ exposure alone. Decreased activities were recorded after acute metal exposures even at high Ca 2+ concentration. There were variable alterations in ATPase activities whereas AChE activity was mostly altered by Hg 2+ exposure. ATPase and AChE activities were changed in both durations due to metal type and Ca 2+ concentration differences. However, ATPase responses were more affected. Changes in the activities demonstrated the sensitivity of these vital enzymes towards environmental factors. Therefore, there is a potential using of these enzymes as biomarkers on the basis of multi-biomarker attitude in environmental toxicology studies.

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Cited by 7 publications

References 51 publications

Morphological and Functional Alterations Induced by Two Ecologically Relevant Concentrations of Lead on Danio rerio Gills

Morphological and Functional Alterations Induced by Two Ecologically Relevant Concentrations of Lead on Danio rerio Gills

Mixture Toxicity of Three Unconventional Gas Fracking Chemicals, Barium, O‐Cresol, and Sodium Chloride, to the Freshwater Shrimp Paratya australiensis

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