2015
DOI: 10.1371/journal.pone.0145210
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Walking the Line: A Fibronectin Fiber-Guided Assay to Probe Early Steps of (Lymph)angiogenesis

Abstract: Angiogenesis and lymphangiogenesis are highly complex morphogenetic processes, central to many physiological and pathological conditions, including development, cancer metastasis, inflammation and wound healing. While it is described that extracellular matrix (ECM) fibers are involved in the spatiotemporal regulation of angiogenesis, current angiogenesis assays are not specifically designed to dissect and quantify the underlying molecular mechanisms of how the fibrillar nature of ECM regulates vessel sprouting… Show more

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Cited by 16 publications
(14 citation statements)
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“…FN1 is produced by pro-angiogenic macrophages 64 and stimulates endothelial cell proliferation, micro-vessel elongation and angiogenesis 65 . Finally, and in line with our observation of extensive lymphangiogenesis in mice, FN1 is an excellent substrate for lymphatic endothelial cells invasion 66 , while TNC induces lymphangiogenesis in tumors 67 . Interestingly, several studies have targeted FN1 over-expression in tumors, with therapeutic molecules or even imaging agents 68,69 .…”
Section: Discussionsupporting
confidence: 86%
“…FN1 is produced by pro-angiogenic macrophages 64 and stimulates endothelial cell proliferation, micro-vessel elongation and angiogenesis 65 . Finally, and in line with our observation of extensive lymphangiogenesis in mice, FN1 is an excellent substrate for lymphatic endothelial cells invasion 66 , while TNC induces lymphangiogenesis in tumors 67 . Interestingly, several studies have targeted FN1 over-expression in tumors, with therapeutic molecules or even imaging agents 68,69 .…”
Section: Discussionsupporting
confidence: 86%
“…Migration speed and persistence of MEFs was assessed on manually pulled plasma fibronectin fibers, based on a previously developed system [ 32 , 76 ]. Fibronectin fibers were pulled from solution (0.4 mg/mL plasma fibronectin in PBS) and deposited on silicone sheets (SMI .005” NRV G/G 40D, Specialty Manufacturing, Saginaw, MI) that were cut to fit a 4-well LabTek chamber (#155382, Thermo Scientific).…”
Section: Methodsmentioning
confidence: 99%
“…We could see more blood vessels in the FN precoating group. These new blood vessels might come from the proliferation of endothelial cells and the differentiation of ESCs, which could be promoted by FN precoating [25].…”
Section: Discussionmentioning
confidence: 99%