Walnut (Juglans spp.)

Rodrı́guez, Roberto; Revilla, A.; Albuerne, M.; Perez, C Alexander

doi:10.1007/978-3-642-61535-1_7

Cited by 17 publications

(5 citation statements)

References 33 publications

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“…The micropropagation of walnut trees presents some difficulties that have to be solved before this technology can be used as a commercial tool for clonal propagation of selected clones (McGranahan et al, 1987;Rodriguez et al, 1989; Gruselle and Boxus, 1990). The main difficulties are: i) the establishment of in vitro shoot-tip cultures from adult walnut trees, ii) uneven and low rates of multiplication or rooting and iii) high number of losses during acclimatization of plantlets to soil.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of walnut trees (Juglans regia L) and response to arbuscular mycorrhizal inoculation

Dolcet-Sanjuan¹,

Claveria²,

Camprubí³

et al. 1996

Agronomie

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Section: Introductionmentioning

confidence: 99%

Micropropagation of walnut trees (Juglans regia L) and response to arbuscular mycorrhizal inoculation

Dolcet-Sanjuan¹,

Claveria²,

Camprubí³

et al. 1996

Agronomie

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“…To meet the increasing need for plants, in vitro vegetative propagation has been intensively studied in several laboratories. In spite of recent progress, rooting of multiplied shoots has remained a difficult step in micropropagation (Rodriguez et al 1989). Somatic embryogenesis is an alternative means of propagation and has the potential to produce complete plantlets directly (Tulecke & McGranahan 1985).…”

Section: Introductionmentioning

confidence: 99%

Maturation and germination of walnut somatic embryos

Deng¹,

Cornu²

1992

Plant Cell Tiss Organ Cult

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Walnut somatic embryos were multiplied by repetitive embryogenesis on a solid basal DKW medium at 25°C in the dark. When the embryos were isolated at early cotyledonary stage (1-2 mm long) from the primary embryos and cultured on the medium for 3 weeks, they developed into mature embryos showing white, enlarged cotyledons and shoot and root apex. After transfer to light on solid germination medium, however, few mature embryos (0-5%) germinated. Germination percentage increased to about 10% when the mature embryos were pretreated by a storage at 4°C in the dark for 2 months, or by desiccation at 25°C in the dark for 3 or 5 days under an air-humidity conditioned by saturated salt solutions (Mg(NO3)2.6HzO, or ZnSO4.7H20 ). Similar results were obtained by the addition of gibberellic acid (GA3) to the germination medium. When mature embryos were desiccated and then placed on medical cotton compresses in liquid germination medium, 45% of the embryos germinated into complete plantlets. These plantlets continued their growth after transplanting to a mixture of peat and vermiculite in pots.

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“…The 8.87 µM BAP level has been reported to be optimal for shoot proliferation of the Prunus cerasus x Prunus munsoniana rootstocks (Dalzoto and Docampo, 1997). Rodriguez et al (1998) on the walnut tree Juglans spp. and Akbas et al (2009) on Amygdalus communis L. noticed that the smallest percentages of sprouting and development of the shoots are due to the strong concentrations of BAP which inhibit them.…”

Section: )mentioning

confidence: 99%

Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration

Mansseri-Lamrioui¹,

Louerguioui²,

Bonaly³

et al. 2011

Afr. J. Biotechnol.

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Determination of the most optimal type and concentration of plant growth regulators as medium constituents is one of the most important aspects of successful micro propagation, among other in vitro factors. With the aim of optimization of in vitro multiplication of wild cherry, the effect of the following cytokinins was studied: 6-benzyladenine (BAP), 2-isopentenyl adenine (2iP) and kinetin (Kin) at concentrations of 1, 2, 4 and 8 mg. l-1. Stem segments of seedlings from juvenile and adult materials were disinfected and grown on a Quoirin and Lepoivre (1977) (QL) medium without growth regulators for 4 weeks. Each material responded differently to the tested cytokinins. The use of 6-benzyladenine resulted in the highest percentage of sprouting, the development of shoots and the ratios of multiplication for two materials of Prunus avium L. In the next experiment, seedlings from the juvenile and adult materials were grown on (MS2/5) medium in the presence of auxins indole-3-butyric acid (IBA), naphtaleneacetic acid (NAA), indole-3-acetic acid (IAA), when compared with concentrations of 0.5, 1, 2, and 4 mg. l-1. For the type of explants and its reactivity with the type and the concentration of auxin, significant differences among explants for root induction were observed. The adult material did not develop roots in any of the auxin and concentration used. In the case of the juvenile material, the IBA was distinguished from the other auxins tested and the highest induction of roots took place in 1 mg. l-1. The most significant induction of cal characterizes, especially, the mediums containing the NAA followed by the IAA with concentrations of 2 and 4 mg. l-1 , respectively, which block the emergence of the roots partly and decreases the rate of rooting thereafter. The highest average number of roots and the highest average length of roots were obtained with the IBA with 1 mg. l-1 .

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Walnut (Juglans spp.)

Cited by 17 publications

References 33 publications

Micropropagation of walnut trees (Juglans regia L) and response to arbuscular mycorrhizal inoculation

Micropropagation of walnut trees (Juglans regia L) and response to arbuscular mycorrhizal inoculation

Maturation and germination of walnut somatic embryos

Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration

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