2021
DOI: 10.1101/2021.12.08.471726
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Wanted not, wasted not: Searching for non-target taxa in environmental DNA metabarcoding by-catch

Abstract: Metabarcoding of environmental DNA is based on primers that are specific to the target taxa (e.g. bacteria, zooplankton, fishes). However, due to the nature of the commonly used protocols, regardless of the chosen primers, several sequences of non-target species will inevitably be generated, but are usually discarded in commonly used bioinformatics pipelines. These non-target sequences might contain important biological information about the presence of other species in the studied habitats and its potential f… Show more

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Cited by 2 publications
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“…As with several metabarcoding primers, the MiFish primers are known to amplify other nontarget organismal groups, including mammal and bird species (Ritter et al, 2022). In our study, we further examined those zOTUs that received a poor taxonomic annotation.…”
Section: Reference Database Construction: Specific Considerationsmentioning
confidence: 99%
“…As with several metabarcoding primers, the MiFish primers are known to amplify other nontarget organismal groups, including mammal and bird species (Ritter et al, 2022). In our study, we further examined those zOTUs that received a poor taxonomic annotation.…”
Section: Reference Database Construction: Specific Considerationsmentioning
confidence: 99%