Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin I in whole blood samples

Zhao, Huan; Su, Enben; Huang, Li; Zai, Yunfeng; Liu, Yuan; Chen, Zhu; Song, Li; Jin, Lian; Deng, Yan; He, Nongyue

doi:10.1016/j.cclet.2021.07.017

Cited by 69 publications

(16 citation statements)

References 27 publications

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“…All vital cellular and organismal functions, including immunological responses and cell communication, depend on proteins [ 103 , 104 , 105 ]. Numerous studies have been done on abnormal protein production in the early stages of illness [ 106 , 107 ]. Additionally, LC droplet-based biosensors for the detection of proteins and peptides have been created.…”

Section: Overview Of Lc Droplet-based Biosensors For Poc Diagnosis Of...mentioning

confidence: 99%

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Xie

et al. 2022

Biosensors

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The development of biosensing platforms has been impressively accelerated by advancements in liquid crystal (LC) technology. High response rate, easy operation, and good stability of the LC droplet-based biosensors are all benefits of the long-range order of LC molecules. Bioprobes emerged when LC droplets were combined with biotechnology, and these bioprobes are used extensively for disease diagnosis, food safety, and environmental monitoring. The LC droplet biosensors have high sensitivity and excellent selectivity, making them an attractive tool for the label-free, economical, and real-time detection of different targets. Portable devices work well as the accessory kits for LC droplet-based biosensors to make them easier to use by anyone for on-site monitoring of targets. Herein, we offer a review of the latest developments in the design of LC droplet-based biosensors for qualitative target monitoring and quantitative target analysis.

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Section: Overview Of Lc Droplet-based Biosensors For Poc Diagnosis Of...mentioning

confidence: 99%

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Xie

et al. 2022

Biosensors

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“…Optical biosensors, becoming increasingly crucial in the sensing field [ 161 ], have been given a new design concept thanks to the advent of nanotechnology [ 162 , 163 , 164 , 165 ]. The combination of optical biosensors and nanotechnology [ 166 , 167 ] enables the traditional biosensor to detect the target more sensitively [ 168 ] and dramatically improves the intensity and durability of the detected signal [ 169 ].…”

Section: Janus Particles For Optical Poctmentioning

confidence: 99%

Application of Janus Particles in Point-of-Care Testing

et al. 2022

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Janus particles (JPs), named after the two-faced Roman god, are asymmetric particles with different chemical properties or polarities. JPs have been widely used in the biomedical field in recent years, including as drug carriers for targeted controlled drug release and as biosensors for biological imaging and biomarker detection, which is crucial in the early detection and treatment of diseases. In this review, we highlight the most recent advancements made with regard to Janus particles in point-of-care testing (POCT). Firstly, we introduce several commonly used methods for preparing Janus particles. Secondly, we present biomarker detection using JPs based on various detection methods to achieve the goal of POCT. Finally, we discuss the challenges and opportunities for developing Janus particles in POCT. This review will facilitate the development of POCT biosensing devices based on the unique properties of Janus particles.

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“…Chemiluminescence (CL) detection technology has completely avoided the shortcomings of high fluorescent signal background and easy to quench, which is becoming the most important area of biological analysis research and development ( Yang et al, 2014 ; Huang; Zhang et al, 2021 ; Hu et al, 2022 ; Liu et al, 2022 ; Zhao et al, 2022 ). Also, the CL signal of antibody joint with typical horseradish peroxidase (HRP), Alkaline phosphatase (ALP) and magnetic beads (MBs) platforms have been perfectly employed to commercialized immunoassay diagnosis kits and effectively solving clinical problems (Huang; Speletas et al, 2020 ; Huang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Clustered Regularly Interspaced Short Palindromic Repeats-Associated Proteins13a combined with magnetic beads, chemiluminescence and reverse transcription-recombinase aided amplification for detection of avian influenza a (H7N9) virus

Peng

et al. 2023

Front. Bioeng. Biotechnol.

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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and Clustered Regularly Interspaced Short Palindromic Repeats-Associated Proteins (CRISPR-Cas) have promising prospects in the field of nucleic acid molecular diagnostics. However, Clustered Regularly Interspaced Short Palindromic Repeats-based fluorescence detection technology is mainly hindered by proteins with conjugated double bonds and autofluorescence, resulting in high fluorescence background, low sensitivity and incompatible reaction systems, which are not conducive to automatic clinical testing. Chemiluminescence (CL) detection technology has been applied mainly owing to its greatly high sensitivity, as well as low background and rapid response. Therefore, we developed a rapid, ultrasensitive and economical detection system based on Clustered Regularly Interspaced Short Palindromic Repeats-Clustered Regularly Interspaced Short Palindromic Repeats-Associated Proteins 13a combined with magnetic beads (MBs) and chemiluminescence (CL) (Cas13a-MB-CL) to detect Influenza A (H7N9), an acute respiratory tract infectious disease. The carboxyl functionalized magnetic beads (MBs-COOH) were covalently coupled with aminated RNA probe while the other end of the RNA probe was modified with biotin. Alkaline phosphatase labeled streptavidin (SA-ALP) binds with biotin to form magnetic beads composites. In presence of target RNA, the collateral cleavage activity of Cas13a was activated to degrade the RNA probes on MBs and released Alkaline phosphatase from the composites. The composites were then magnetically separated followed by addition of ALP substrate Disodium 2-chloro-5-{4-methoxyspiro [1,2-dioxetane-3,2′-(5′-chloro) tricyclo (3.3.1.13,7) decan]-4-yl}-1-phenyl phosphate (CDP-star), to generate the chemiluminescence signal. The activity of Associated Proteins 13a and presence of target RNA was quantified by measuring the chemiluminescence intensity. The proposed method accomplished the detection of H7N9 within 30 min at 25°C. When combined with Reverse Transcription- Recombinase Aides Amplification (RT-RAA), the low detection limit limit of detection was as low as 19.7 fM (3S/N). Our proposed MB-Associated Proteins 13a-chemiluminescence was further evaluated to test H7N9 clinical samples, showing superior sensitivity and specificity.

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Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin I in whole blood samples

Cited by 69 publications

References 27 publications

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Application of Janus Particles in Point-of-Care Testing

Clustered Regularly Interspaced Short Palindromic Repeats-Associated Proteins13a combined with magnetic beads, chemiluminescence and reverse transcription-recombinase aided amplification for detection of avian influenza a (H7N9) virus

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