Wasp venom immunotherapy induces a shift from IL‐4‐producing towards interferon‐gamma‐producing CD4<sup>+</sup>and CD8<sup>+</sup>T lymphocytes

Schuerwegh, Annemie J.; Clerck, Luc S. De; Bridts, Chris H.; Stevens, W. J.

doi:10.1046/j.1365-2222.2001.01066.x

Cited by 24 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the end of a 5‐day semi‐rush VIT, there was a significant decrease in the percentages of IL‐4‐producing CD4 + and CD8 + T cells, compared with cytokine‐producing cells before VIT. After 6 months of VIT, a shift from IL‐4‐producing towards IFN‐γ‐producing CD4 + and CD8 + T lymphocytes has been reported previously, in experiments in which Treg cells have not been intensely studied [59]. During bee VIT, IL‐10 serum levels increase from the second day of the build‐up phase [56].…”

Section: Introductionmentioning

confidence: 71%

Mechanisms of immunotherapy to wasp and bee venom

Özdemir¹,

Küçüksezer

Akdiş³

2011

Clin Experimental Allergy

View full text Add to dashboard Cite

Hymenoptera venoms are important allergens that can elicit both local and systemic allergic reactions, including life-threatening anaphylaxis. Venom immunotherapy (VIT) remains the most effective treatment, reducing the risk of systemic reactions in individuals with Hymenoptera venom allergy. VIT can restore normal immunity against venom allergens and provide patients with a lifetime of tolerance to venoms. During VIT, peripheral tolerance is induced by the generation of allergen-specific regulatory T (Treg) cells, which suppress proliferative and cytokine responses against the venom allergens. Treg cells are characterized by IL-10 secretion that directly or indirectly influence effector cells of allergic inflammation, such as mast cells, basophils and eosinophils. Treg cells also have influence on B cells, suppressing IgE production and inducing the production of blocking type IgG4 antibodies against venom allergens. An accumulating body of evidence suggests that Treg cells may affect allergen sensitization and methods for enhancing this cell population may eventually improve the efficacy of VIT. In this article, immune mechanisms enrolled in bee and wasp VIT are reviewed.

show abstract

Section: Introductionmentioning

confidence: 71%

Mechanisms of immunotherapy to wasp and bee venom

Özdemir¹,

Küçüksezer

Akdiş³

2011

Clin Experimental Allergy

View full text Add to dashboard Cite

show abstract

“…In particular, a shift in cytokine responses from a Th2 to a Th1 pattern was demonstrated during rush VIT using both HB [45] and YJ venom [46]. Regarding T-reg, a recent study found an elevated IL-10 production by CD3(+) T cells few hours after rush VIT [47]. In our study, patients were treated with aqueous extracts of Hymenoptera venom during the incremental phase and well tolerated the shift to the maintenance dose with aluminum hydroxide-adsorbed extracts, confirming previous data [32, 48].…”

Section: Discussionmentioning

confidence: 99%

Hymenoptera Venom Immunotherapy: Tolerance and Efficacy of an Ultrarush Protocol versus a Rush and a Slow Conventional Protocol

Florio

Giuliano²,

Oricchio³

et al. 2012

Journal of Allergy

View full text Add to dashboard Cite

Background and Objective. Various venom immunotherapy (VIT) protocols are available for Hymenoptera allergy. Although adverse reactions (ADRs) to VIT are widely reported, controlled trials are still needed. We conducted a randomized prospective study to evaluate ADRs and the efficacy of three VIT regimens. Methods. 76 patients with Hymenoptera allergy, aged 16–76 years, were randomized to receive an ultrarush protocol (group A: 27 patients), a rush protocol (group B: 25), or a slow protocol (group C: 24). Aqueous venom extract was used in incremental phase and an adsorbed depot in maintenance phase. ADRs and accidental Hymenoptera stings during VIT were used to evaluate efficacy. Results. During incremental treatment, ADRs occurred in 1.99%, 3.7%, and 3.9% of patients in groups A, B, and C, and in 0.99%, 1.46%, and 2.7%, respectively, during maintenance. ADRs were significantly fewer in group A (incremental + maintenance phase) than in group C (1.29% versus 3.2%; P = 0.013). Reactions to accidental Hymenoptera stings did not differ among groups (1.1%, 1.2%, and 1.1%). Conclusion. Ultrarush was as effective as the rush and slow protocols and was associated with a low incidence of reactions to stings. This study indicates that ultrarush VIT is a valid therapeutic option for Hymenoptera allergy.

show abstract

“…As previously described (Schuerwegh et al, 2001), isolated uterine lymphocyte cells were stained for 30 min in the dark at room temperature with 1 mL of specific monoclonal antibodies directed against T-cell surface markers, including APC-conjugated anti-CD3 (553066, BD Biosciences, San Diego, CA), FITC-conjugated anti-CD4 (553046, BD Biosciences, San Diego, CA), and PEconjugated anti-CD8 (553032, BD Biosciences, San Diego, CA). Next, the lymphocytes were washed with 0.2 mL PBS and centrifuged at 500 Â g for 5 min (three times).…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation

Liu

Dong

Wang

et al. 2014

Stress

View full text Add to dashboard Cite

The influence of stress on embryo implantation is not well understood. Prior studies have focused on later gestational stages and the long-term impact of stress on immune function. The objective of this study is to investigate the effects of restraint stress on the immune parameters and the oxidative states of the uterus during implantation. In this study, pregnant CD1 mice were subjected to restraint stress (4 h/d) on embryonic day 1 (E1) and sacrificed on E3, E5, and E7. Maternal plasma corticosterone (CORT) secretion and implantation sites in the uterus were examined. The uterine (excluding embryos) homogenate and uterine lymphocytes were collected to examine oxidative stress states and associated immune parameters. The results demonstrated that restraint stress increased maternal plasma CORT secretion and reduced the number of implantation sites by 15.3% on E5 and by 26.1% on E7. Moreover, restraint stress decreased the density of uterine natural killer (uNK) cells in the endometrium by 22.1-47.9% and increased the density of mast cells in the myometrium by 55.6-76.9%. Restraint stress remarkably decreased the CD3(+)CD4(+) T/CD3(+)CD8(+) T cell ratio (by 26.2-28.9%) and attenuated uterine lymphocyte proliferation and secretion of cytokines. In addition, restraint stress threatened the intracellular equilibrium between oxidants and antioxidants, resulting in decreased glutathione peroxidase (GSH-PX) (32.2% and 45.7%), superoxide dismutase (SOD) (15.5% and 26.1%), and total antioxidant capacity (T-AOC) (18.4% and 18.2%) activities and increased malondialdehyde (MDA) (34.4% and 43.0%) contents on E5 and E7. In conclusion, these findings demonstrate that restraint stress causes abnormal implantation and negatively impacts immune parameters in association with oxidative stress in mice.

show abstract

Wasp venom immunotherapy induces a shift from IL‐4‐producing towards interferon‐gamma‐producing CD4⁺and CD8⁺T lymphocytes

Abstract: Venom immunotherapy induced a shift from IL-4-producing towards IFN-gamma-producing CD4+ as well as CD8+ T lymphocytes.

Cited by 24 publications

References 41 publications

Mechanisms of immunotherapy to wasp and bee venom

Mechanisms of immunotherapy to wasp and bee venom

Hymenoptera Venom Immunotherapy: Tolerance and Efficacy of an Ultrarush Protocol versus a Rush and a Slow Conventional Protocol

Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation

Contact Info

Product

Resources

About

Wasp venom immunotherapy induces a shift from IL‐4‐producing towards interferon‐gamma‐producing CD4+and CD8+T lymphocytes

Abstract: Venom immunotherapy induced a shift from IL-4-producing towards IFN-gamma-producing CD4+ as well as CD8+ T lymphocytes.

Cited by 24 publications

References 41 publications

Mechanisms of immunotherapy to wasp and bee venom

Mechanisms of immunotherapy to wasp and bee venom

Hymenoptera Venom Immunotherapy: Tolerance and Efficacy of an Ultrarush Protocol versus a Rush and a Slow Conventional Protocol

Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation

Contact Info

Product

Resources

About

Wasp venom immunotherapy induces a shift from IL‐4‐producing towards interferon‐gamma‐producing CD4⁺and CD8⁺T lymphocytes