“…A methanogen identified as Methanosphaera , an archaeon previously found to be involved in fat, oil, and grease methanogenesis [ 68 ], was detected with comparable relative abundances among all the anaerobic consortia (1.3–1.7 %). This finding indicates that despite the observed synergistic effect of TW80 and FeSO 4 on biogas production, their combined addition did not significantly change the microbial composition in the consortia relative to the control or the consortia with either of the added chemicals alone.…”