In this study, the response surface approach was employed to predict and optimize the biodiesel yield. Blend of Karanja and Chlorella vulgaris oil was used in a volumetric ratio of 75:25 to lower the free fatty acid content. The biodiesel production process parameters from this hybrid oil were optimized through a single-step transesterification process. The optimized conditions resulted in a maximum biodiesel yield of 97.55% . These conditions included a catalyst amount of 1.09% (w/w), a reaction time of 91.47 minutes, a reaction temperature of 56.86°C, and a methanol-to-oil molar ratio of 8.46:1. The experimental biodiesel yield was compared with the predicted response obtained from the response surface methodology (RSM). The comparison demonstrated good agreement between the experimental and predicted values.