Wastewater Sample Collection - Moore Swab and Grab Sample Methods v1

Abstract

This protocol describes materials and methods that can be employed in the field to collect Moore swabs and/or grab samples for wastewater sample collection. The materials and methods described herein are meant to provide guidelines, examples, and tips for various methods of wastewater collection; however, these techniques can be adapted and modified depending on the setting and goals of the particular project or program. This protocol makes note of composite sampling instructions but is focused on situations where installation and operation of a composite sampler is not feasible.

Correlation of SARS-CoV-2 in Wastewater and Individual Testing Results in a Jail, Atlanta, Georgia, USA

“…A sampling team from Emory University in Atlanta collected weekly wastewater samples from the jail throughout the project period. Moore swabs ( Figure 1 ) were suspended overnight in manhole sites around the jail property ( Figure 2 ) ( 21 , 22 ). Eluted wastewater from the swabs was tested by using quantitative real-time reverse transcription PCR (qRT-PCR) at the Center for Global Safe Water, Sanitation, and Hygiene Laboratory at Emory University, as described ( 21 , 23 ).…”

Wastewater Surveillance for SARS-CoV-2 in an Atlanta, Georgia Jail: A study of the feasibility of wastewater monitoring and correlation of building wastewater and individual testing results

“…An Emory University sampling team collected weekly wastewater samples from the jail through the project period. Moore swabs (see Figure 1) were suspended overnight in manhole sites around the jail property (Figure 2) [23,24]. Eluted wastewater from the swabs was tested using real time, quantitative reverse transcription-polymerase chain reaction (RT-qPCR) at the Center for Global Safe WASH Laboratory of Emory as previously described [25,26].…”