Watching neuronal circuit dynamics through functional multineuron calcium imaging (fMCI)

Takahashi, Naoya; Sasaki, Takuya; Usami, Atsushi; Matsuki, Norio; Ikegaya, Yuji

doi:10.1016/j.neures.2007.03.001

Cited by 77 publications

(66 citation statements)

References 36 publications

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“…The advantage of this technique is that large populations of neurones can be loaded with the preferred calcium indicator allowing the investigation of a large number of neurones simultaneously ( [15,32]; for review of techniques see [10,36]). …”

Section: Calcium Imaging In Dcn Brain Slicesmentioning

confidence: 99%

Activation of muscarinic receptors increases the activity of the granule neurones of the rat dorsal cochlear nucleus—a calcium imaging study

Kőszeghy

Vincze

Rusznák

et al. 2012

Pflugers Arch - Eur J Physiol

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Acetylcholine modulates the function of the cochlear nucleus via several pathways. In this study the effects of cholinergic stimulation were studied on the cytoplasmic Ca 2+ concentration of granule neurones of the rat dorsal cochlear nucleus (DCN). Ca 2+ transients were recorded in Oregon-Green-BAPTA 1-loaded brain slices using a calcium imaging technique. For the detection, identification, and characterisation of the Ca 2+ transients, a wavelet analysis-based method was developed. Granule cells were identified on the basis of their size and localisation. The action potential-coupled character of the Ca 2+ transients of the granule cells was established by recording fluorescence changes and electrical activity simultaneously. Application of the cholinergic agonist carbamyl-choline (CCh) significantly increased the frequency of the Ca 2+ transients (from 0.37 to 6.31 min -1 , corresponding to a 17.1-fold increase; n = 89).This effect was antagonised by atropine, whereas CCh could still evoke an 8.3-fold increase of the frequency of the Ca 2+ transients when hexamethonium was present.Using immunolabelling, the expression of both type 1 and type 3 muscarinic receptors (M1 and M3 receptors, respectively) was demonstrated in the granule cells. Application of 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide (an M3-specific antagonist) prevented the onset of the CCh effect, whereas an M1-specific antagonist (pirenzepine) was less effective. We conclude that cholinergic stimulation increases the activity of granule cells, mainly by acting on their M3 receptors. The modulation of the firing activity of the granule cells, in turn, may modify the firing of projection neurones, and may adjust signal processing in the entire DCN.

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Section: Calcium Imaging In Dcn Brain Slicesmentioning

confidence: 99%

Activation of muscarinic receptors increases the activity of the granule neurones of the rat dorsal cochlear nucleus—a calcium imaging study

Kőszeghy

Vincze

Rusznák

et al. 2012

Pflugers Arch - Eur J Physiol

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“…Furthermore, it was shown that under the conditions of a mature network when spontaneous Ca 2+ cell activity is low while maintaining the conduction of excitation by adding the neural network of excitatory neurotransmitters cause strict synchronization of cell activity. In the papers [4,10,11,13] aimed at studying the neural networks in the hippocampus was also shown that about 60% of the neurons responded to sensory irritation, due to feature in the structure of the hippocampus.…”

Section: Dynamics Of Spontaneous Camentioning

confidence: 99%

“…Meaning of calcium signaling system in the brain is very large, because it is directly involved in the regulation of fundamental processes of neural integration, regulates various localized functional responses, and maintains the set of intracellular signaling pathways. Calcium signaling is the subject of many scientific research areas related to the study of the role of neurons and astrocytes in the functioning of the central nervous system (CNS) in health and disease [1][2][3][4][5][6][7]. Metabolism is impossible at millimolar levels of intracellular Ca 2+ , so all living cells retain the Ca 2+ concentration at the low nanomolar level, thus creating a difference in gradients between cellular compartments and intercellular space [8,9].…”

mentioning

confidence: 99%

“…Network Mechanisms in the Hippocampus CA3 Region in Rat Postnatal Development during the activation of ionotropic and metabotropic receptors, channels, regulates by the transporter proteins and probably depends on the stage of ontogenesis [3,4]. Hippocampus -the structure of the central nervous system, which is involved in the mechanisms of emotion and memory consolidation.…”

mentioning

confidence: 99%

“…One of these is the network of neurons in the CA3 field. This network receives inputs from cells of the entorhinal cortex and the dentate gyrus, moreover CA3 pyramidal neurons form the connection between themselves and interneurons, forming a closed network that operates in conditions of acute slice and generates spontaneous Ca 2+ activity [4,11,13,14]. Therefore, to estimate the age dependence of Ca 2+ activity in the cells were investigated Ca 2+ oscillations in neuronal and glial networks and the interactions between them.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Intracellular Calcium Network Activity in the Hippocampus CA3 Region in Rat Postnatal Development

Mitaeva¹,

Mozherov²,

Kastalskiy³

et al. 2016

Sovrem Tehnol Med

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Hippocampus -the structure of the central nervous system, which is involved in the mechanisms of memory consolidation. The hippocampus has a certain topology distribution of cellular elements, which provides the many cellular networks. One of them is the network of neurons in the CA3 field. In this article discusses the features of the Ca 2+ signaling system of cells of CA3 field of rat hippocampus of early (P5-8, P14-16) and late (P21-25) postnatal development in different physiological conditions: spontaneous activity, in violation of the excitation in the neural network by adding tetrodotoxin (Ca 2+ signaling), as well as the effects of excitatory neurotransmitters (ATP, L-glutamate). This work extends the concepts of Ca 2+ signaling in rat hippocampus cells of early and late stages of postnatal ontogenesis. The study showed that changes in Ca 2+ activity in the cells CA3 field of rat hippocampal taking place during the neonatal period of postnatal ontogenesis directly related to the functioning of neural networks and the metabolic state of the cells.

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High‐Speed Multineuron Calcium Imaging Using Nipkow‐Type Confocal Microscopy

et al. 2011

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Conventional confocal and two-photon microscopy scan the field of view sequentially with single-point laser illumination. This raster-scanning method constrains video speeds to tens of frames per second, which are too slow to capture the temporal patterns of fast electrical events initiated by neurons. Nipkow-type spinning-disk confocal microscopy resolves this problem by the use of multiple laser beams. We describe experimental procedures for functional multineuron calcium imaging (fMCI) based on Nipkow-disk confocal microscopy, which enables us to monitor the activities of hundreds of neurons en masse at a cellular resolution at up to 2000 fps.

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Watching neuronal circuit dynamics through functional multineuron calcium imaging (fMCI)

Cited by 77 publications

References 36 publications

Activation of muscarinic receptors increases the activity of the granule neurones of the rat dorsal cochlear nucleus—a calcium imaging study

Activation of muscarinic receptors increases the activity of the granule neurones of the rat dorsal cochlear nucleus—a calcium imaging study

Intracellular Calcium Network Activity in the Hippocampus CA3 Region in Rat Postnatal Development

High‐Speed Multineuron Calcium Imaging Using Nipkow‐Type Confocal Microscopy

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