1978
DOI: 10.1016/0014-5793(78)80963-6
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Water and calcium ions in cell fusion induced by poly(ethylene glycol)

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Cited by 108 publications
(50 citation statements)
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“…Freeze-fracture electron microscopy revealed numerous morphological features, some of which are known to occur in lipids 10 to 20% hydrated (Kleman et al, 1977). This dehydration effect agrees with previous studies which show that no unbound water exists in solutions of PEG-6,000 above 45% (wt/vol) (Blow et al, 1978). Only 11 of the 23 water molecules per phospholipid molecules are said to remain at the presence of 47% PEG (Arnold et al, 1983).…”
Section: Discussionsupporting
confidence: 86%
“…Freeze-fracture electron microscopy revealed numerous morphological features, some of which are known to occur in lipids 10 to 20% hydrated (Kleman et al, 1977). This dehydration effect agrees with previous studies which show that no unbound water exists in solutions of PEG-6,000 above 45% (wt/vol) (Blow et al, 1978). Only 11 of the 23 water molecules per phospholipid molecules are said to remain at the presence of 47% PEG (Arnold et al, 1983).…”
Section: Discussionsupporting
confidence: 86%
“…These values also agree with observations of the disappearance of a differential scanning calorimetry (DSC) melting exortherm of frozen free water at 45% PEG-6,000. (Blow et al, 1978). Our finding that complete fusion occurs at 38% PEG and above thus correlates with the percentages of PEG required to completely bind water.…”
Section: Discussionsupporting
confidence: 58%
“…An important known property of PEG is its ability to bind and structure water (Baran, Solomentseva, Mank & Kurilenko, 1972;Blow et al, 1978;Tilcock & Fisher, 1979). This property appears to be related to a critical PEG concentration (45-50%, wt/wt) for fusion to occur (Davidson & Gerald, 1977).…”
mentioning
confidence: 98%
“…see [1,2]). By which properties o f PEG solutions the necessary cell altera tions are induced was still an open question [1,3]. In this paper I demonstrate, how in ionfree fusogenic PEG solutions PEG can be replaced in part by carbohydrates an d /o r salts.…”
mentioning
confidence: 99%