Water Bridging Dynamics of Polymerase Chain Reaction in the Gauge Theory Paradigm of Quantum Fields

Abstract

We discuss the role of water bridging the DNA-enzyme interaction by resorting to recent results showing that London dispersion forces between delocalized electrons of base pairs of DNA are responsible for the formation of dipole modes that can be recognized by Taq polymerase. We describe the dynamic origin of the high efficiency and precise targeting of Taq activity in PCR. The spatiotemporal distribution of interaction couplings, frequencies, amplitudes, and phase modulations comprise a pattern of fields which constitutes the electromagnetic image of DNA in the surrounding water, which is what the polymerase enzyme actually recognizes in the DNA water environment. The experimental realization of PCR amplification, achieved through replacement of the DNA template by the treatment of pure water with electromagnetic signals recorded from viral and bacterial DNA solutions, is found consistent with the gauge theory paradigm of quantum fields.The enzymatic synthesis of DNA is a major subject of molecular biology, to which a large number of studies have been devoted. The elucidations of the double-helix structure of DNA and its scaffolding by water and the hydrogen bonding of its bases in a planar configuration, directly opened the way to understanding DNA duplication. It is

Guided Folding of Life’s Proteins in Integrate Cells with Holographic Memory and GM-Biophysical Steering

“…In this respect, some innovative papers with designed and properly controlled experiments, suggested that electromagnetic fields may reflect macromolecular features of enzymes like DNA-polymerase in water, and that this provides structure specific information storage in aqueous solutions [82] [83] [84]. The electromagnetic radiative properties of DNA and enzyme macromolecules likely depend on the characterization of their electric dipoles.…”

“…It has been argued that London force dipoles in such intra-protein hydrophobic pockets could couple together and oscillate coherently, thus generating a radiative field. Montagnier recently described the fundamental role of water dipole dynamics bridging the interaction between DNA and enzymatic proteins (see Figure 7) so as to allow polymerase chain reaction (PCR) processes to occur [84]. They describe the dynamic origin of the high efficiency and precise targeting of Taq activity in PCR.…”

Addendum: Montagnier, L.; Aïssa, J.; Capolupo, A.; Craddock, T.J.A.; Kurian, P.; Lavallee, C.; Polcari, A.; Romano, P.; Tedeschi, A.; Vitiello, G. Water Bridging Dynamics of Polymerase Chain Reaction in the Gauge Theory Paradigm of Quantum Fields. Water 2017, 9, 339

“…The authors wish to make the following addendum to their paper [1]: After the publication of our paper, submitted on 11 February 2017, we read the paper "DNA's Chiral Spine of Hydration", by M. Luke McDermott, Heather Vanselous, Steven A. Corcelli, and Poul B. Petersen, published in ACS Central Science, doi:10.1021/acscentsci.7b00100, on 24 May 2017 [2], where the authors report their discovery of a chiral water superstructure surrounding DNA under ambient conditions. This is the first observation of a chiral spine of hydration templated by a biomolecule and has been obtained by use of chiral sum frequency generation (SFG) spectroscopy, a method analogous to circular dichroism measurements.…”

Collective behavior of oscillating electric dipoles

“…As all biomolecules in real biological environment are in watery solution, we have to take into account the presence of surrounding water molecules. Though recent studies reveal that the water in biological system can have a highly non trivial behaviour with respect to electrodynamic fields generated by the electric dipole of biomolecules [12][13][14][15][16], in this article we will assume the surrounding water to play simply the role of a thermal bath.…”