Water Extract of Fermented New Korean Medicinal Mixture (F-MAPC) Controls Intracellula Adipogenesis and Glut-4 dependent Glucose Uptake in 3T3-L1 Adipocytes and L6 Myoblasts

Abstract

Objectives : The aim of this study was to investigate the effects water extract of fermented new korean medicinal mixture, combinations of Mori Folium, Adenophorae Radix, Phllostachyos Folium and Citri Pericarpium (F-MAPC), on adipocyte differentiation, adipogenesis and glucose uptake using undiffernentiated 3T3-L1 adipocytes and L6 myoblasts. Methods : Each herb and those mixture were respectively fermented and then extracted with water. We carried on MTT assay for check-up on cell toxicity, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. Western blot analysis for measurement of pAMPK and pACC, C/EBPα, PPARγ and Glut-4 protein expressions were performed. Results : F-MAPC showed significant inhibitory activity on adipocyte differentiation in 3T3-L1 preadipocytes without affecting cell toxicity as assessed by measuring fat accumulation, and this effect was 2 fold higher in 0.2 mg/ml F-MAPC than that of the same dose of each fermented herbal extract alone. In addition, these effects were associated with modulation of adipogenic transcription factors, such as C/EBPα, PPARγ, as well as stimulated phosphorylations of AMPK and ACC. Translocation of Glut-4 was significantly increased by 10.2% in L6 cells treated with 0.2 mg/ml F-MAPC compared with that of control.Conclusions : These results demonstrate that F-MAPC may be an ideal candidate for therapy of obesity and diabetes by disturbing the differentiation into adipocytes, as well as the inducement of intramuscular glucose uptake from blood.

Potentials of Chenpi on Metabolic Syndrome: A Review

“…Phosphorylated Akt transports glucose into intracellular space, by bumping transporter 4 (GLUT4) to membrane cell 48 . As well, GLUT4 is a glucose transporter that is activated by insulin, and related influx of glucose into cells 49 . In L6 myotubes, CMPW 50, 100, 200, 500 μg/ml up-regulated IRS-1, PIK3R and GLUT4 mRNA expression in all groups, dose-dependently.…”