“…Samples were gently stirred and w45 g was weighed into centrifuge tubes (50 mL), tempered (10 C for 10 min) in the centrifuge (Heraeus BioFuge S tratos, Thermo Electron Corporation, Waltham, MA, USA), and centrifuged at 5000Â g for 15 min, 10 C (Harwalkar & Kalab, 1983;Kneifel, Paquin, Abert, & Richard, 1991). The supernatant was thoroughly removed with a pipette, and the relative amount of separated whey was calculated as the mass ratio of supernatant to the weighed in sample (w45 g).…”