:The maintaining of drinking water quality, access to clean urban surface water are the most challenge resulting in water-borne diseases. Although, the developing countries, the Geographical Information System (GIS) technology has not been yet systematically utilized. Therefore, the present study aimed to establish the parameters for assessing surface water quality and how they would cause health issues in the study area. This study reveals the surface water quality variations by using principal component analysis (PCA) techniques with the help of Landsat 8 Operational Land Imager (OLI) satellite imagery. Regular monitoring of environmental quality database produced by (GIS) could manage information from various sources such as, domestic, industrial, recreational activities, point and non-points sources etc. This monitoring activity could make spatial correlations with epidemiology data about time and space distribution of water-borne diseases. The spatial correlation of water contamination tank and it's surroundings of land use / land cover (LULC) activities were calculated by the technique of buffer analysis. Using Medical GIS, could easily detect the circulation and spread of disease across the geographic regions which could be used for planning, policy making, water resource protection and avoid contamination. The study area also included the industrial and residential areas of Madurai urban region, Tamil Nadu, India, to validate ground truth verification. The study would help to reveal the disease monitoring, surveillance systems, improving the distribution of health resources by predicting available health care accessibility, the source of pollution and it impacts on public health. Especially in Madurai need more attention to epidemiology. Because, the 11,132 epidemic disease affected cases were recorded in 2019 [1]. In every year government of India spends more money for this type of epidemiological spread; especially in Tamil Nadu spend 8868 lakhs of money. WHO also revealed that the developing countries are highly affected by the epidemic diseases.