Water‐in‐ionic liquid microemulsion‐based organogels as novel matrices for enzyme immobilization

Pavlidis, Ioannis V.; Tzafestas, Kyriakos; Stamatis, Haralambos

doi:10.1002/biot.201000052

Cited by 23 publications

(15 citation statements)

References 45 publications

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“…MEs are thermodynamically stable isotropic mixtures of polar, nonpolar, and amphiphilic phase, which provide unique advantages like nanosized aggregates, pharmacodynamic stability, enhanced drug solubilization, and ease of preparation (10,11). Depending on the polarity, ILs can be employed to prepare ionic liquid in oil (IL/ o) (12,13) or ionic liquid in water (14,15) type of MEs.…”

Section: Introductionmentioning

confidence: 99%

Development of Novel Ionic Liquid-Based Microemulsion Formulation for Dermal Delivery of 5-Fluorouracil

et al. 2014

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Abstract. The present study was aimed at synthesizing an imidazole-based ionic liquid 1-butyl-3-methylimidazolium bromide (BMIMBr) and subsequent development of a novel ionic liquid-in-oil (IL/o) microemulsion (ME) system for dermal delivery of a poorly permeating drug 5-fluorouracil (5-FU). A significant enhancement in the solubility of 5-FU was observed in BMIMBr. IL/o MEs of 5-FU were prepared using isopropyl myristate, Tween 80/Span 20, and BMIMBr. Results of ex vivo skin permeation studies through mice skin indicated that the selected IL/o ME exhibited 4-fold enhancement in percent drug permeation as compared to aqueous solution, 2.3-fold as compared to hydrophilic ointment, and 1.6-fold greater permeation than water in oil (w/o) ME. The results of in vivo studies against dimethylbenz(a)anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mice skin carcinogenesis demonstrated that the IL/o ME could effectively treat skin cancer in 4 weeks. In addition, the side effects such as erythema and irritation associated with the conventional formulations were not observed. Histopathological studies showed that the use of IL/o ME caused no anatomic and pathological changes in the skin structure of mice. These studies suggest that the use of IL-based ME system can efficiently enhance the solubility and permeability of 5-FU and hence its therapeutic efficacy.KEY WORDS: 5-fluorouracil; dermal delivery; ionic liquids; ionic liquid in oil (IL/o) microemulsion; skin cancer.

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Section: Introductionmentioning

confidence: 99%

Development of Novel Ionic Liquid-Based Microemulsion Formulation for Dermal Delivery of 5-Fluorouracil

et al. 2014

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“…Moreover, compared with other methods, the use of MBGs is a relatively inexpensive and facile method of enzyme immobilization. Although successful biotransformations employing enzymes immobilized in MBGs have been well documented [6], [7] and were highlighted in our previous study [8], few reports have focused on the application of this method to kinetic resolution reactions.…”

Section: Introductionmentioning

confidence: 93%

Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability

Zhang

Jia

Wang

et al. 2015

Biotechnology Reports

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“…Therefore, among several approaches to resolve the challenges, one of the most effective methods is immobilization of the enzymes within an aqueous microenvironment in the organic solvents. Microemulsions formed by amphiphilic surfactants have been widely reviewed as effective media for the immobilization of enzymes in hydrophobic solvents (Itabaiana et al 2014;Pavlidis et al 2010;Uskokovi and Drofenik 2007). The MBGS method based on microemulsions has been used to form matrices for enzyme immobilization to achieve enzymatic catalysis in nonconventional medium as they appear to be rigid and stable for a long time, even within the reaction solution (Zhang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Function Of Surfactants In Immobilization of Cellulase And Multiphase Hydrolysis: A Review

Wang

De-yi

Wang

et al. 2021

Preprint

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Surfactants, especially non-ionic surfactants, play an important role in the preparation of nanocarriers and can also promote the enzymatic hydrolysis of lignocellulose. A broad overview of the current status of surfactants on the immobilization of cellulase is provided in this review. In addition, the restricting factors in cellulase immobilization in the complex multiphase hydrolysis system are discussed, including the carrier structure characteristics, solid-solid contact obstacles, external diffusion resistance, limited recycling frequency, and invalid combination of enzyme active centers. Furthermore, promising prospects of cellulase-oriented immobilization are proposed, including the hydrophilic-hydrophobic interaction of surfactants and cellulase in the oil-water reaction system, the reversed micelle system of surfactants, and the possible oriented immobilization mechanism.

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Water‐in‐ionic liquid microemulsion‐based organogels as novel matrices for enzyme immobilization

Abstract: HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

Cited by 23 publications

References 45 publications

Development of Novel Ionic Liquid-Based Microemulsion Formulation for Dermal Delivery of 5-Fluorouracil

Development of Novel Ionic Liquid-Based Microemulsion Formulation for Dermal Delivery of 5-Fluorouracil

Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability

Function Of Surfactants In Immobilization of Cellulase And Multiphase Hydrolysis: A Review

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