Abstract:Purpose: This investigation examined the effects of Acqua Lete® bicarbonate calcic mineral water ingestion on blood lactate, glucose, and serum lactate dehydrogenase.Methods: 88 amateur male athletes underwent two experimental trials with modified Wingate test: the first was carried out without hydration (Test C), the second (Test H) with hydration following this scheme: 44 subjects (Group A) hydrated by a very low mineral content water and 44 subjects (Group B) hydrated by Acqua Lete ® . Measures of body temperature, [La -], glucose, total LDH and its isoenzyme pattern, were taken before (T0), during (T1-T5), and after exercise (T6-T7).Results: Blood lactate significantly increased after the session of exercises in both groups: after hydration and 30' of resting (T7) Group B returned a level of lactate lower than the Group A (2.2±0.2 vs 2.9±0.3mmol/L; p<0.001). In Test H, LDH activities after exercise did not change but serum LDH5 isoenzyme activity decreased by 0.9% in athletes in Group B compared to Group A (p< 0.05).Conclusions: All the athletes (Group B) hydrated pre-exercise with Acqua Lete® showed a significant decrease in blood lactate levels post-exercise and changes in LDH isoenzymatic pattern compared with athletes hydrated pre-exercise with a low mineral content water.