Water relations of polyol accumulation by Zygosaccharomyces rouxii in continuous culture

Zyl, Petrus Jakobus van; Prior, Bernard A.

doi:10.1007/bf00170561

Cited by 27 publications

(8 citation statements)

References 25 publications

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“…1E), increase in biomass yield on oxygen (data not shown), and respiratory coefficients greater than 1.0 (data not shown). In contrast to our results, Van Zyl and Prior (1990) did not observe any ethanol production by Z. rouxii under aerobic conditions. They assumed, in common with Brown and Edgley (1980), that Z. rouxii does not display the Crabtree effect.…”

Section: Experimental Study With Z Rouxiicontrasting

confidence: 86%

Estimation of steady‐state culture characteristics during acceleration‐stats with yeasts

Sluis

Westerink

Dijkstal

et al. 2001

Biotech & Bioengineering

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Steady-state culture characteristics are usually determined in chemostat cultivations, which are very time-consuming. In contrast, acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed with a constant acceleration rate are not so time-consuming, especially at high acceleration rates. Therefore, the A-stat could be advantageous to use instead of the chemostat. However, the highest acceleration rate, meaning the fastest A-stat that can be applied for estimating steady-state culture characteristics, is not known yet. Experimental results obtained with Zygosaccharomyces rouxii, an important yeast in soy sauce processes, showed that the culture characteristics during the A-stat with an acceleration rate of 0.001 h(-2) were roughly comparable to those of the chemostat. For higher acceleration rates the deviation between the culture characteristics in the A-stat and those in the chemostat obtained at the same dilution rate generally started to increase. The source of these deviations was examined by simulation for Saccharomyces cerevisiae. The simulations demonstrated that this deviation was not only dependent on the metabolic adaptation rate of the yeast, but also on the rate of change in environmental substrate concentrations during A-stats. From this work, it was concluded that an A-stat with an acceleration rate of 0.001 h(-2) is attractive to be used instead of chemostat whenever a rough estimation of steady-state culture characteristics is acceptable.

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Section: Experimental Study With Z Rouxiicontrasting

confidence: 86%

Estimation of steady‐state culture characteristics during acceleration‐stats with yeasts

Sluis

Westerink

Dijkstal

et al. 2001

Biotech & Bioengineering

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“…Indeed, during continuous cultures of Zygosaccharomyces rouxii at dilution rates in the range 0.05-0.2 h −1 , the maximum biomass concentration at steady state, reached for the lowest dilution rate, was only 1.6 g L −1 , obtained in media at low water activity (0.96) induced by NaCl addition (Van Zyl and Prior 1990). Accounting for the low cellular density recorded during culture in media at low water activity, an important part of the assimilated glucose is used for polyols production and for energy supply, needed to maintain the required amount of intracellular polyols, which allows osmotic adjustment to be ensured (Djelal et al 2005).…”

Section: Discussionmentioning

confidence: 98%

Continuous culture for the bioproduction of glycerol and ethanol by Hansenula anomala growing under salt stress conditions

et al. 2011

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Continuous cultures of Hansenula anomala were carried out in media at low water activity, resulting from NaCl addition. Three dilution rates were compared and it was shown that a clear steady state was only recorded for the lowest dilution rate tested (0.03 h −1 ), leading to a biomass concentration of 4.3 g L −1 . At 0.06 h −1 dilution rate, steady state was maintained for no more than 20 h and a clear washout was observed at 0.12 h −1 . The experimental ratio ethanol on glycerol produced at steady state (D=0.03 h −1 ) was similar to that previously recorded in batch culture in similar conditions after 30 h of growth, 7.8 and 7.9, respectively. The comparison of continuous culture to repeated batch culture was clearly in favour of the continuous culture, which led to significantly higher glycerol and ethanol production rates, 4.5 and 4 times higher than those recorded in repeated batch culture, respectively, even if significant amounts of glucose remained in the bleeding of the bioreactor.

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“…The role of the secondary polyol in osmoregulation is less clear. The arabitol concentration in Z. rouxii grown in a continuous culture under osmotic stress depended on the solute used to adjust the a w , although the glycerol concentration remained similar (Van Zyl & Prior, 1990). This pointed to the possibility that the secondary polyol might have additional functions apart from osmotic adjustment.…”

Section: Discussionmentioning

confidence: 99%

The osmotic stress tolerance of basidiomycetous yeasts

Tekolo

McKenzie

Botha

et al. 2010

FEMS Yeast Research

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The growth and accumulation of intracellular polyols at reduced water activity (a(w)) were studied in 40 basidiomycetous yeast strains. The growth of most strains showed greater tolerance to NaCl than sorbitol at the same a(w). No strain was able to grow below 0.90a(w). (13)C nuclear magnetic resonance spectroscopy revealed that glycerol was the major solute accumulated intracellularly by all the yeasts when grown to 0.96a(w) (NaCl). Arabitol or mannitol was also accumulated in some yeasts, whereas a few only accumulated glycerol. Analysis of six yeasts in detail revealed that the intracellular glycerol concentrations of five yeasts increased significantly when grown at 0.96a(w) (NaCl or sorbitol) compared with growth at 0.998a(w). Arabitol and mannitol concentrations also increased, but not to the same degree. Intracellular potassium concentrations decreased when grown at 0.96a(w) (NaCl or sorbitol) and sodium increased, but only when grown at 0.96a(w) (NaCl). The survival of nine strains was evaluated in soil cultures and it was found that all grew at 100% field capacity, whereas at lower field capacity, only some strains grew or survived. The growth of basidiomycetous yeasts appears to be more sensitive to reduced a(w) than ascomycetous yeasts.

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Water relations of polyol accumulation by Zygosaccharomyces rouxii in continuous culture

Cited by 27 publications

References 25 publications

Estimation of steady‐state culture characteristics during acceleration‐stats with yeasts

Estimation of steady‐state culture characteristics during acceleration‐stats with yeasts

Continuous culture for the bioproduction of glycerol and ethanol by Hansenula anomala growing under salt stress conditions

The osmotic stress tolerance of basidiomycetous yeasts

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