Water-soluble fraction of petroleum induces genotoxicity and morphological effects in fat snook (Centropomus parallelus)

Medeiros, Laila Carine Campos; Delunardo, Frederico Augusto Cariello; Simões, Larissa Novaes; Paulino, Marcelo Gustavo; Vargas, Thais Souza; Fernandes, Marisa Narciso; Scherer, Rodrigo; Chippari-Gomes, Adriana Regina

doi:10.1016/j.ecoenv.2017.06.031

Cited by 15 publications

(10 citation statements)

References 36 publications

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“…Micronuclei quantification was performed according to Al-Sabti and Metcalfe (1995) and Medeiros et al (2017), using a Giemsa stain (5% w/v). One thousand erythrocytes per fish were analyzed under an optical microscope (100x magnification).…”

Section: Genotoxicity Assaysmentioning

confidence: 99%

“…Only intact cells with distinct nuclear and cellular membranes were scored. The alkaline Comet assay was performed as described by Tice et al (2000), Cariello Delunardo et al (2013) and Medeiros et al (2017) using silver nitrate. The Blood samples were diluted 1:120 (v:v) in RPMI 1640 medium for the alkaline comet assay.…”

Section: Genotoxicity Assaysmentioning

confidence: 99%

“…The Blood samples were diluted 1:120 (v:v) in RPMI 1640 medium for the alkaline comet assay. All sides were blind-reviewed, and the extent of DNA was quantified by the lengh of DNA migration (Tice et al, 2000;Cariello Delunardo et al, 2013;Medeiros et al, 2017). A total of 100 cells from each fish were randomly analyzed using optical microscopy (100x magnification) to measure the length of the comet's tail (Tice et al, 2000;Cariello Delunardo et al, 2013;Medeiros et al, 2017).…”

Section: Genotoxicity Assaysmentioning

confidence: 99%

“…All sides were blind-reviewed, and the extent of DNA was quantified by the lengh of DNA migration (Tice et al, 2000;Cariello Delunardo et al, 2013;Medeiros et al, 2017). A total of 100 cells from each fish were randomly analyzed using optical microscopy (100x magnification) to measure the length of the comet's tail (Tice et al, 2000;Cariello Delunardo et al, 2013;Medeiros et al, 2017). The DNA damage was classified into four classes according to Kobayashi et al (1995) as follow: (o) no damages, (1) short tail smaller than the diameter of the nucleus, (2) a tail length 1-2 fold the diameter of the nucleus and (3) a tail length > 2-fold the diameter of the nucleus.…”

Section: Genotoxicity Assaysmentioning

confidence: 99%

“…In recent years several studies have evaluated the impact of metals as genotoxic pollutants. At the molecular level, the comet assay and the micronucleus test (MN) are complementary biomarkers for genotoxic testing and indicate significant DNA damage based on two different endpoints (Rudolf and Cervinka, 2006;Villela et al, 2006;Medeiros et al, 2017). Micronuclei arise from chromosomal fragments or whole chromosomes that are not incorporated into daughter nuclei at mitosis (Schmid, 1975) and could be easily visualised in peripheral erythrocytes (Omar et al, 2012), whereas the alkaline comet assay measures strand breaks before the DNA repair systems intervene (Medeiros et al, 2017;Scalon et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Genotoxicity effects on Geophagus brasiliensis fish exposed to Doce River water after the environmental disaster in the city of Mariana, MG, Brazil

et al. 2019

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The Doce River basin has suffered the largest environmental accident ever occurred in Brazil with the influx of tailings from Fundão and Santarém, belonging to Samarco mining company, due to the disaster in Mariana. A spill between 50 and 60 million m3 of tailings was estimated by the company. According to Samarco, the wastewater was composed mainly of clay, silt and heavy metals like iron, copper and manganese. Thereby, the objective of the present study was evaluated the genotoxic damage in juvenile of Geophagus brasiliensis (Quoy e Gaimard, 1824) exposed to Doce river water before (DRWBA – Doce River water before acident) and after (DRWAA – Doce River water after acident) the influx of tailings from the Germano and Santarém Dam disasters in Mariana, MG, Brazil. For this, 24 individuals of the species G. brasiliensis (obtained on IFES/ALEGRE fish culture) were submitted to a bioassay with three treatments and eight replicates. The treatments were: 1) Control water (water from the urban water supply system, filtered with a 0.45 µm membrane), 2) DRBA and 3) DRAA. After 96 h, these fishes were anesthetized to remove blood for evaluation of genotoxic damage (micronucleus and comet). For the bioassay, a total of 80 L of The Doce River water were collected before the influx of tailings and after the influx and then submitted to metal quantification analysis. Fish exposed to DRWBA and DRWAA treatments showed a significant increase in both the number of erythrocyte micronuclei and the DNA damage index in relation to the control fish; however, they did not present any differences between the two treatments. The results demonstrate that the DRWBA treatment was already genotoxic for the fish, mainly due to dissolved Cu concentrations in the water. The DRWAA treatment probably presented genotoxicity due to the increase in the dissolved fraction and synergistic effects of several metals found in the tailings of the Mariana accident.

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Section: Genotoxicity Assaysmentioning

confidence: 99%