Water-soluble Hemin-mPEG-enhanced Luminol Chemiluminescence for Sensitive Detection of Hydrogen Peroxide and Glucose

Abstract

In the present study, we synthesized a water-soluble substance (Hemin-mPEG) at room temperature by using hemin and poly(ethylene glycol) methyl ether (mPEG). It was found that the Hemin-mPEG maintained the excellent catalytic activity inherited from hemin, and was first used to catalyze a luminol-H2O2 chemiluminescence (CL) system to generate an intense and slow CL signal. The results of a mechanism research showed that the presence of Hemin-mPEG could promote the production of oxygen-relative radicals from H2O2 and dissolved oxygen in solution. Based on this mechanism, an ultra-sensitive, cheap and simply practical sensor for detecting glucose and H2O2 was developed. Under the most optimal experimental conditions, H2O2 and glucose detection results exhibited a good linear range from 0.002 to 3 μM and from 0.02 to 4 μM, respectively, and the detection limits were 1.8 and 10 nM, respectively. This approach has been successfully used to detect glucose in actual biological samples, and achieved good results.

Hydrogen Peroxide Sensors for Biomedical Applications

“…Before we are able to understand H 2 O 2 ' s role in the body, there must be reliable, fast, and versatile methods of detection with appropriate range and detection limits to function within biological systems and to detect biologically-relevant concentrations. In the past five years, researchers have continued to improve existing H 2 O 2 sensors [26,27,33,34,36,52,57,65,66,71,76] and developed novel methods of H 2 O 2 detection [26,27,32,66,67]. Sensor developments have occurred in the improvement of H 2 O 2 detection limits, with researchers able to detect much smaller concentrations than in the past; longevity, with researchers developing sensors that function over multiple weeks/months; and cost, with prices dropping in sensor development, this making sensors easier to manufacture at desired price points [65,66,71].…”

“…In 2019, Wang et al decreased the H 2 O 2 detection limit of the luminol reaction via the synthesis and use of a hemin and poly(ethylene glycol) methyl ether catalyst [36]. The new catalyst resulted in a detection limit of 1.8 nM [36], lower than other luminol-H 2 O 2 detection levels [31,36,[43][44][45].…”

“…H2O2 sensors that could be used by researchers, including those outlined below [10,25,[28][29][30][31][32][33][34][35][36][37][38]. light that can then be read to determine H2O2 concentration [16].…”

Core-shell Au@Pt Nanoparticles Catalyzed Luminol Chemiluminescence for Sensitive Detection of Thiocyanate

“…It is well known that the weak CL emission of lumino-H2O2 is due to the transition from the excited state of the 3-aminophthalate anions (3-APA *) to the ground state. 29,30 In order to acquire the enhancement mechanism of Au@Pt NPs on luminol-H2O2 reaction, the CL spectra were investigated. As shown in Fig 3b, the position of the maximum emission wavelength was located at 425 nm in each case, which was in agreement with the typical peak of luminol CL, between SCNand Pt layers.…”

“…Experimental conditions: SCNions was 0.3 µM, other ions were 0.03 mM, Na2C2O4 was 1.2 mM.Analytical SciencesAdvance Publication by J-STAGE Received December 17, 2019; Accepted February 14, 2020; Published online on February 28, 2020 DOI: 10.2116/analsci.19P475Fig. 7(a) When SCNin different concentrations (0,5,10,15,20,25,30, 60, 90, 120, 150, 180 nM) existed in Au@Pt NPs-luminuol-H2O2 system, CL kinetic curves of SCNwere detected.The black curve was the CL kinetic curve for the reaction of luminol + H2O2, (b) Calibration curve of SCN -. Experimental conditions: luminol and H2O2 reaction time was 4 h, 3.5 mM luminol in 0.1 M NaOH solution, Au@Pt NPs was 0.3 nM, H2O2 was 10 mM.…”