Water-soluble polysaccharides isolated from skin juice, gel juice and flower of Aloe vera Miller

Chang, Xiulian; Chen, B.Y.; Feng, Yimin

doi:10.1016/j.jtice.2010.07.007

Cited by 49 publications

(30 citation statements)

References 18 publications

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“…The average molecular weight information was calculated in a Microsoft Excel worksheet using the data extracted from the gel-permeation profile and molecular weight calibration curve (Chang et al, 2011a).…”

Section: Resultsmentioning

confidence: 99%

“…ferox flower was initially dried at 70℃ in an oven and ground using a food processor. These dried powders (100 g) were suspended in 500 mL of distilled water and refluxed scribed in our previous paper (Chang et al, 2011a).…”

Section: Methodsmentioning

confidence: 99%

“…In order to upgrade the byproduct, skin and flower of A. ferox might be as an alternative low-cost resource of aloe polysaccharides potentially used in the production of aloe drink, aloe jelly, food additive and various other aloe products. In this work, we attempt to elicit information that contributes to the understanding of compositional features of the polysaccharides from A. ferox different tissues and compared with the result reported by Mabusela et al (1990) and with those of A. vera and A. arborescens species in the context of our previous researches (Chang et al, 2011a(Chang et al, , 2011b, which is therefore essential to the development of a better use for this agricultural byproduct. …”

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“…ferox flower was initially dried at 70℃ in an oven and ground using a food processor. These dried powders (100 g) were suspended in 500 mL of distilled water and refluxed 902 Water Soluble Polysaccharides Separated from Aloe scribed in our previous paper (Chang et al, 2011a).All measurements were done in triplicate except for monosaccharides analysis that was done in duplicates and the measured values are shown as the respective mean value. …”

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“…Then, the ratio of V e /V o was plotted against the logarithm of their respective peak maxima molecular weight (M p ). The calibration curve of log M p of standard dextrans on GPC against their elution volume (V e ) was obtained (log M p = −0.0541 V e + 6.044, correlation coefficient r = 0.9998) and the peak molecular weight was calculated by the calibration curve.The average molecular weight information was calculated in a Microsoft Excel worksheet using the data extracted from the gel-permeation profile and molecular weight calibration curve (Chang et al, 2011a).Chemical analysis of the polysaccharide Total carbohydrate content and uronic acid were determined by the phenol-H 2 SO 4 method (Dubois et al, 1956) and carbazole reactions, using glucose and glucuronic acid as the standard, respectively.The purity of the crude polysaccharides was identified by Sepharose 4B (Amersham Biosciences) gel permeation chromatography (42 cm × 1 cm). And then, the fractions (2.0 mL) were collected.…”

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Research on Water Soluble Polysaccharides Separated from Skin Juice, Gel Juice and Flower of Aloe ferox Miller

Chang

Han

Wang

et al. 2013

FSTR

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Crude water-soluble polysaccharides were isolated from gel juice, skin juice and flowers of Aloe ferox Miller. Result indicated that skin juice contained 15 fold the level of polysaccharides in gel juice from one plant, strongly suggesting the potential industrial application of A. ferox skin as the resource of polysaccharides, rather than discarded. The amount of polysaccharides in the flower of A. ferox was about 2 fold compared with those of A. arborescens and A. vera. After fractionation by anion-exchange chromatography and gel permeation chromatography, gel juice polysaccharide was separated into three neutral (GN1, 1.7%; GN2, 17.6% and GN3, 0.4%) and one acid (GA1, 19.7%) fractions. Skin juice polysaccharide consisted of one neutral (SN1, 25.2%) and two acid (SA1, 7.2%; SA2, 18.0%) fractions. Flower polysaccharide consisted of one neutral (FN1, 1.6%) and one acid (FA1, 26.4%) fractions. All the subfractions have relatively high polydispersity indices (1.0 to 2.5). Sugar analysis shows that the subfractions are constituted predominantly by galactose, glucose and mannose with different mole percentages, except that acidic flower polysaccharide was composed of two more monosaccharides, rhamnose and xylose, and neutral skin juice polysaccharide yielded glucose as the sole monosaccharide. Galacturonic acid was identified as the sole uronic acid in all acidic polysaccharides from different tissues.Keywords: Aloe ferox, skin juice, gel juice, flower, polysaccharides, composition *To whom correspondence should be addressed. E-mail: yongmeif@126.com IntroductionThere are over 250 species of aloe grown world over. Out of wide range of aloe species, A. vera industry is a wellresearched, well-established, multi-billion dollar industry (Reynolds and Dweck, 1999). A. arborescens is much used in Japan in a way similar to A. vera, although in these studies it appears that the whole leaf was used in the preparation rather than the isolated gel (Reynolds and Dweck, 1999). A. vera and A. ferox are economically important with a significant international trade (Oldfield, 1993). A. ferox, the Cape Aloe or Bitter Aloe, is used to produce bitters as laxative in South Africa which had an estimated export value of approximately $1.2 million in 1996, now close to $2 million (Dickson, 2008). The mucilaginous gel is used in skin preparation and cosmetics (Sachedina and Bodeker, 1999).It has multiple pharmacological effects on healing wounds (Jia et al., 2008), skin cancer, burns and psoriasis (Maliehe, 1997;Van Wyk et al., 2009;Loots et al., 2007), eczema (Van Wyk and Gericke, 2000), constipation (Wintola et al., 2010). Currently, value adding such as the production of cosmetics or beverages is done mainly in export destinations (Melin, 2009). Kleinschmidt (2004) described the health benefits of beverages and fortified food products containing the leaf parenchyma of A. ferox, although it has been difficult to compete with the A. vera products available in the international market. Locally, the production of aloe drinks from polysaccha...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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