Water-soluble superparamagnetic manganese ferrite nanoparticles for magnetic resonance imaging

Yang, Hong; Zhang, Cuixia; Shi, Xiangyang; Hu, He; Du, Xiaoxia; Fang, Yong; Ma, Yu‐Lan; Wu, Huixia; Yang, Shiping

doi:10.1016/j.biomaterials.2010.01.055

Cited by 245 publications

(108 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The internalization of the Gd-Au DENPs potentially occurs through two distinct mechanisms, phagocytosis and diffusion via cell walls, in agreement with the literature. 29,30 Furthermore, the TEM data also revealed that the incubation of the Gd-Au DENPs with an Au concentration as high as 50 μM did not markedly affect the cell morphology, corroborating with the MTT assay and optical microscopy observation data.…”

Section: In Vitro Cellular Uptake Of Gd-au Denpssupporting

confidence: 75%

Multifunctional dendrimer-based nanoparticles for in vivo MR/CT dual-modal molecular imaging of breast cancer

Li¹,

Wen²,

Larson³

et al. 2013

IJN

Self Cite

View full text Add to dashboard Cite

Development of dual-mode or multi-mode imaging contrast agents is important for accurate and self-confirmatory diagnosis of cancer. We report a new multifunctional, dendrimer-based gold nanoparticle (AuNP) as a dual-modality contrast agent for magnetic resonance (MR)/computed tomography (CT) imaging of breast cancer cells in vitro and in vivo. In this study, amine-terminated generation 5 poly(amidoamine) dendrimers modified with gadolinium chelate (DOTA-NHS) and polyethylene glycol monomethyl ether were used as templates to synthesize AuNPs, followed by Gd(III) chelation and acetylation of the remaining dendrimer terminal amine groups; multifunctional dendrimer-entrapped AuNPs (Gd-Au DENPs) were formed. The formed Gd-Au DENPs were used for both in vitro and in vivo MR/CT imaging of human MCF-7 cancer cells. Both MR and CT images demonstrate that MCF-7 cells and the xenograft tumor model can be effectively imaged. The Gd-Au DENPs uptake, mainly in the cell cytoplasm, was confirmed by transmission electron microscopy. The cell cytotoxicity assay, cell morphology observation, and flow cytometry show that the developed Gd-Au DENPs have good biocompatibility in the given concentration range. Our results clearly suggest that the synthetic Gd-Au DENPs are amenable for dual-modality MR/CT imaging of breast cancer cells.

show abstract

Section: In Vitro Cellular Uptake Of Gd-au Denpssupporting

confidence: 75%

Multifunctional dendrimer-based nanoparticles for in vivo MR/CT dual-modal molecular imaging of breast cancer

Li¹,

Wen²,

Larson³

et al. 2013

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…No significant induction of cytotoxicity was shown on HeLa cells treated with tetraethylene glycol stabilized nanoparticles up to a concentration of 200 µg/mL. 16 The surface charge of water-soluble manganese ferrite nanoparticles was shown to influence their uptake and toxicity on murine macrophages, with cationic particles being more biologically reactive. 18 Recently, we proposed a high-yield, low-cost mechanochemical process to synthesize manganese ferrite (MnFe 2 O 4 ) nanoparticles, which were subsequently functionalized with citric acid and stabilized in ferrofluid form.…”

Section: Introductionmentioning

confidence: 95%

“…13,14 Superparamagnetic manganese ferrite (MnFe 2 O 4 ) nanoparticles are particularly attractive as candidate contrast agents for magnetic resonance imaging, because of a very high magnetization due to their large magnetic spin. 15,16 Since a fundamental aspect of drug development is the trade-off evaluation of risks and benefits, a parallel toxicological assessment of these new medical devices is in progress, especially in view of the possible specific biological reactivity of nanosized materials that has recently been emerging. 17 Until now, few data have been published.…”

Section: Introductionmentioning

confidence: 99%

Biodistribution and acute toxicity of a nanofluid containing manganese iron oxide nanoparticles produced by a mechanochemical process

Bellusci¹,

Barbera²,

Padella³

et al. 2014

IJN

View full text Add to dashboard Cite

Superparamagnetic iron oxide nanoparticles are candidate contrast agents for magnetic resonance imaging and targeted drug delivery. Biodistribution and toxicity assessment are critical for the development of nanoparticle-based drugs, because of nanoparticle-enhanced biological reactivity. Here, we investigated the uptake, in vivo biodistribution, and in vitro and in vivo potential toxicity of manganese ferrite (MnFe 2 O 4 ) nanoparticles, synthesized by an original high-yield, low-cost mechanochemical process. Cultures of murine Balb/3T3 fibroblasts were exposed for 24, 48, or 72 hours to increasing ferrofluid concentrations. Nanoparticle cellular uptake was assessed by flow-cytometry scatter-light measurements and microscopy imaging after Prussian blue staining; cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony-forming assays. After a single intravenous injection, in vivo nanoparticle biodistribution and clearance were evaluated in mice by Mn spectrophotometric determination and Prussian blue staining in the liver, kidneys, spleen, and brain at different posttreatment times up to 21 days. The same organs were analyzed for any possible histopathological change. The in vitro study demonstrated dose-dependent nanoparticle uptake and statistically significant cytotoxic effects from a concentration of 50 μg/mL for the MTT assay and 20 μg/mL for the colony-forming assay. Significant increases in Mn concentrations were detected in all analyzed organs, peaking at 6 hours after injection and then gradually declining. Clearance appeared complete at 7 days in the kidneys, spleen, and brain, whereas in the liver Mn levels remained statistically higher than in vehicle-treated mice up to 3 weeks postinjection. No evidence of irreversible histopathological damage to any of the tested organs was observed. A comparison of the lowest in vitro toxic concentration with the intravenously injected dose and the administered dose of other ferrofluid drugs currently in clinical practice suggests that there might be sufficient safety margins for further development of our formulation.

show abstract

“…Manganese ferrite have spinel structure and its properties depend on morphology and size, which can be controlled by synthesis parameters [1]. Various methods are used to produce MnFe 2 O 4 nanoparticles, such as solid state reactions [2], combustion synthesis [3], thermal decomposition [4], coprecipitation [5] and hydrothermal method [6].…”

Section: Introductionmentioning

confidence: 99%

STUDY ON SYNTHESIS OF MnFe2O4/GNPs COMPOSITE AND APPLICATION ON HEAVY METAL REMOVAL

Anh¹

2018

JST

View full text Add to dashboard Cite

Nowadays, composite materials between mixed-metal oxides and graphene are widely studied due to their multiple applications on different fields. MnFe 2 O 4 is a magnetic material which has the ability to absorb toxic heavy metal in water. Graphene nanoplatelets (GNPs) with about 10 layers, is one of type of graphene. GNPs was used as matrix for the fine distribution of metal oxide nanoparticles. Surface area for the absorption process can be increased. Composite was synthesized using solvothermal method, in which mixed-metal oxide nanoparticles were directly formed in-situ from precursor salts onto GNPs surface. Synthesized material was analyzed using XRD, SEM and EDX methods to determine its properties. Heavy metal absorption capacity was also studied and showed good results.

show abstract

Water-soluble superparamagnetic manganese ferrite nanoparticles for magnetic resonance imaging

Cited by 245 publications

References 36 publications

Multifunctional dendrimer-based nanoparticles for in vivo MR/CT dual-modal molecular imaging of breast cancer

Multifunctional dendrimer-based nanoparticles for in vivo MR/CT dual-modal molecular imaging of breast cancer

Biodistribution and acute toxicity of a nanofluid containing manganese iron oxide nanoparticles produced by a mechanochemical process

STUDY ON SYNTHESIS OF MnFe2O4/GNPs COMPOSITE AND APPLICATION ON HEAVY METAL REMOVAL

Contact Info

Product

Resources

About