“…These spectrophotometric methods used for the study of ionic equilibria of pyridoxal-N-methochloride should be applicable to other pyridoxal analogs, as they are synthesized for studies on model systems. For example, pK' values of 3.96 and 8.4 were a spectrophotometrically determined for "alpha^-pyridoxalylacetic acid" by P. Scott Furbish (See Table 1 This information can be used to interpret pH effects on the binding of these coenzymes to apoenzymes and to substrates (Williams and Neilands, 195^;Metzler and Snell, 1955;Snell and Metzler, 1956;Martell, 1963).…”