Water Sorption and Glass Transition of Pig Gastric Mucin Studied by QCM-D

Znamenskaya, Yana; Sotres, Javier; Gavryushov, Sergei; Engblom, Johan; Arnebrant, Thomas; Kocherbitov, Vitaly

doi:10.1021/jp311968b

Cited by 27 publications

(65 citation statements)

References 35 publications

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“…Such mucin coatings can reconstitute levels of surface hydration [ 21,22 ] and lubrication [23][24][25] similar to those found in native mucus. Our data show that the removal of mucin-bound glycans results in a signifi cant reduction in hydration and lubricity of the mucin-coated surface.…”

Section: Doi: 101002/admi201500308mentioning

confidence: 68%

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

Crouzier

Boettcher

Geonnotti

et al. 2015

Adv Materials Inter

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glycoproteins, a family of high molecular weight and densely glycosylated biopolymers. Those mucins can act as an infection shield by trapping various viruses in the biopolymer matrix [ 1 ] and they reduce bacterial adhesion to surfaces. [ 2,3 ] Mucins also form highly hydrated and lubricative layers that protect the underlying epithelium from dehydration and shear stress that emerge during eye blinking or when swallowing food. Water typically accounts for up to 95% of the total mass in the mucus gel. [ 4,5 ] Shifts in the mucus water content correlate with substantial changes of the mucus barrier function [6][7][8] and result in important pathological disorders such as dry mouth [ 9 ] and dry eye. [ 10 ] Mucin-associated glycans contribute up to 80% of the molecular weight of mucins [ 11 ] and contain highly hydrated hydroxyl groups. Differences in the amount and composition of glycans from mucins extracted from pig stomachs and mucins from bovine submaxillary glands correlate with their capacity to adsorb water. [ 12 ] However, other properties such as protein composition also differ between these two mucin species, precluding the establishment of a defi nitive link between the presence of mucin glycans and hydration.Mucins, and other well-hydrated molecules such as zwitterionic polymers, polysaccharides, and polyelectrolytes are generally good boundary lubricants when adsorbed [ 13,14 ] or assembled in brush structures [15][16][17][18] on surfaces. Hence, mucin-associated glycans could also be essential for the lubricative function of mucus. In a related system, the mucin-like macromolecule lubricin showed a signifi cant decrease in lubricity when its associated glycans were removed. [ 19 ] Moreover, the lubricity of salivary fi lms, which contain mucins, has also been correlated with the total amount of glycosylation present in the fi lm. However, also here, the complexity of saliva composition precluded the identifi cation of the specifi c role of mucin glycosylation. [ 20 ] Hence, there is a need for a more defi ned experimental system to determine if mucin glycosylation is essential to the hydration and lubrication properties of mucins.This study explores the role of glycosylation in mucin hydration and lubricity, using mucin coatings as a simplifi ed model system. Such mucin coatings can reconstitute levels of surface hydration [ 21,22 ] and lubrication [23][24][25] similar to those found in native mucus. Our data show that the removal of mucin-bound glycans results in a signifi cant reduction in hydration and lubricity of the mucin-coated surface. We show that the hydration and lubricity of deglycosylated mucins can be partially A key property of mucin glycoproteins is their exceptional capacity to hydrate and lubricate surfaces. In vivo, mucins assemble into mucus hydrogels that cover the epithelium and protect it from dehydration and shear stress. A better understanding of the origin of these properties could lead to new treatment strategies for patients with poor mucus coverage, defective mucus produ...

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Section: Doi: 101002/admi201500308mentioning

confidence: 68%

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

Crouzier

Boettcher

Geonnotti

et al. 2015

Adv Materials Inter

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“…Equation (7) reflects the fact that the concentration of the incoming solution is constant (irrespective of time), while Eq. (8) shows that the concentration of the outgoing solution changes continuously during the sorptiondesorption experiment. For clarity, we revise Eqs.…”

Section: Calculation Of the Water Activitymentioning

confidence: 97%

“…On the other hand, thick films are associated with unstable resonance signals and disappearing of overtones (especially at high levels of hydration). 8 Therefore, to coat the sensors accordingly, we used the following procedures: for spin-coating, the sample was applied by adding 20 µl of aqueous solution and most often repeating the procedure 3-10 times in total during which the sensor was rotated at 1200 rpm (spin-coater developed in-house). The concentration of the aqueous solutions used for spin-coating varied between 2 and 20 wt.…”

Section: B Qcm-d Instrument and Preparation Of Qcm-d Sensorsmentioning

confidence: 99%

“…In many cases, the coating of the quartz sensor with a film leads to deviations from the Sauerbrey equation due to the rheological and/or surface properties of the applied film. In this work, the QCM-D data were analyzed according to the single viscoelastic film in air model, 13 which previously has been used for QCM-D hydration studies on pig gastric mucin, 8 lysozyme, 10 and starch films. 11 According to this model, the frequency change, normalized per overtone, corresponding to the total mass of the coated sensor m f , is proportional to the overtone in square n 2 by the following relation:…”

Section: Mass Determinationmentioning

confidence: 99%

“…For clarity, we revise Eqs. (7) and (8) to describe how the mass of LiCl salt and water changes in the solution kept in bottle A according to the following:…”

Section: Calculation Of the Water Activitymentioning

confidence: 99%

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Humidity scanning quartz crystal microbalance with dissipation monitoring setup for determination of sorption-desorption isotherms and rheological changes

Björklund

Kocherbitov

2015

Review of Scientific Instruments

Self Cite

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A new method to determine water sorption-desorption isotherms with high resolution in the complete range of water activities (relative humidities) is presented. The method is based on quartz crystal microbalance with dissipation monitoring (QCM-D). The QCM-D is equipped with a humidity module in which the sample film is kept in air with controlled humidity. The experimental setup allows for continuous scanning of the relative humidity from either dry to humid conditions or vice versa. The amount of water sorbed or desorbed from the sample is determined from the resonance frequencies of the coated quartz sensor, via analysis of the overtone dependence. In addition, the method allows for characterization of hydration induced changes of the rheological properties from the dissipation data, which is closely connected to the viscoelasticity of the film. The accuracy of the humidity scanning setup is confirmed in control experiments. Sorption-desorption isotherms of pig gastric mucin and lysozyme, obtained by the new method, show good agreement with previous results. Finally, we show that the deposition technique used to coat the quartz sensor influences the QCM-D data and how this issue can be used to obtain further information on the effect of hydration. In particular, we demonstrate that spin-coating represents an attractive alternative to obtain sorption-desorption isotherms, while drop-coating provides additional information on changes of the rheological properties during hydration.

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Advances in Mucin Biopolymer Research: Purification, Characterization, and Applications

Marczynski

Winkeljann

Lieleg

2020

Biopolymers for Biomedical and Biotechnological Applications

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Water Sorption and Glass Transition of Pig Gastric Mucin Studied by QCM-D

Cited by 27 publications

References 35 publications

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

Humidity scanning quartz crystal microbalance with dissipation monitoring setup for determination of sorption-desorption isotherms and rheological changes

Advances in Mucin Biopolymer Research: Purification, Characterization, and Applications

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