Water stress-sensitized photoinhibition in senescing cotyledons of clusterbean: Changes in thylakoid structures and inactivation of photosystem 2

Deo, P. M.; Biswal, U. C.; Biswal, Basanti

doi:10.1007/s11099-006-0005-x

Cited by 12 publications

(3 citation statements)

References 41 publications

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“…The homogenate was filtered through a four layer cheesecloth, then centrifuged (5009g) at 4°C for 2 min to remove residual debris. Isolated chloroplasts in the supernatant were collected by centrifugation again at 4°C and 1,5009g for 10 min, and then washed using the same extract medium (Deo et al 2006). Thylakoid membrane in the pellet was prepared by subjecting the chloroplasts to a small volume of osmotic shock medium (10 mM Hepes-NaOH at pH 7.5, 25 mM KCl).…”

Section: Plant Materials and Thylakoid Membrane Preparationmentioning

confidence: 99%

Factors altering the membrane fluidity of spinach thylakoid as determined by fluorescence polarization

et al. 2011

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Alterations in fluidity of thylakoid membranes isolated from spinach chloroplasts in response to sodium bisulfite (NaHSO 3 ), hydrogen peroxide (H 2 O 2 ), sodium dodecyl sulfate (SDS), bovine serum albumin (BSA), and free linoleic acid (LA) were investigated by means of a fluorescence polarization study with 1,6-diphenyl-1,3,5-hexatriene as the fluorescence probe. A decrease in fluidity and an increase in microviscosity of membrane were caused by NaHSO 3 and H 2 O 2 treatment. In contrast, SDS and BSA were found to increase thylakoid membranes fluidity and decrease microviscosity, in which the corresponding correlation coefficients were -0.9995 to -0.9516 (SDS) and -0.9359 (BSA), respectively. No changes in thylakoid membranes fluidity induced by free LA were found until its concentration above 5 mM where the polarization value (P value) declined (increased fluidity). The results suggest that the changes in thylakoids membrane fluidity might depend on the characteristics, mechanism and extent of the interactions between membrane components and compounds added.

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Section: Plant Materials and Thylakoid Membrane Preparationmentioning

confidence: 99%

Factors altering the membrane fluidity of spinach thylakoid as determined by fluorescence polarization

et al. 2011

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“…Protein degradation in senescent leaves is an important hydrolytic process that plays a crucial role in nutrient recycling, particularly nitrogen. Many genes that are associated with senescence and encode proteases are synthesized de novo or are induced during senescence (Smarth 1994, Deo et al 2006. The reduction in protein content may be related to an imbalance between their synthesis and degradation, which may result in a decrease in the content of enzymes, such as Rubisco, resulting in a reduction in photosynthetic rate and a lower sugar production (Table 2) (Thimann et al 1977, Smarth 1994, Tezara et al 1999.…”

Section: Discussionmentioning

confidence: 99%

Biochemical and morphophysiological strategies of Myracrodruon

Souza¹,

Barbosa²,

Morais³

et al. 2020

Biologia plant.

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In view of the ecological, social, and economic importance of Myracrodruon urundeuva Allemão, the objective of this study was to investigate the strategies of this species under drought during its initial phase of development. Two-monthold plants were cultivated under continuous irrigation or no irrigation for 20 d. After this period, the water-stressed plants were rehydrated for 20 d. Physiological, biochemical, and anatomical variables were evaluated on 20 th and 40 th day. Water deficit (25 and 85 % leaf relative content) caused senescence followed by leaf abscission. Growth in height was negatively affected by water deficit (37 % reduction). A decrease in the thickness of the mesophyll was accompanied by a decrease of the total chlorophyll content. Water deficit affected saccharide metabolism and altered cellular component dynamics. Enzyme activities were higher during the rehydration period than during the water stress. There was no increase in lipid peroxidation in plants subjected to water deficit. A reduction in the stomatal opening during water stress was a strategy of reducing water loss through transpiration.

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“…The work conducted so far to understand the mechanism of damage and adaptation of photosynthetic apparatus of green plants experiencing flooding stress (FS) is meager (Mauchamp and Methy 2004, Panda et al 2006). The photosynthetic apparatus, especially its photosystem (PS) 2 is very sensitive to different abiotic stresses (Strasser and Tsimilli-Michael 2001, 2006, Deo et al 2006. The degree of stress induced damage and stress adaptations can therefore be examined by monitoring the behaviour of the photosystem exposed to FS.…”

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confidence: 99%

Response of senescing rice leaves to flooding stress

Mishra¹,

Patro²,

Mohapatra³

et al. 2008

Photosynt.

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Flooding stress (FS) induced changes in pigment and protein contents and in photochemical efficiency of thylakoid membranes of chloroplasts were investigated during senescence of primary leaves of rice seedlings. Leaf senescence was accompanied by loss in 2,6-dichlorophenolindophenol (DCPIP) photoreduction, rate of oxygen evolution, quantum yield of photosystem 2 with an increase in MDA accumulation, and non-photochemical quenching (NPQ) of chlorophyll fluorescence. These changes were further aggravated when the leaves during this period experienced FS. The increase in NPQ value under stress may indicate photosynthetic adaptation to FS.Additional key words: adaptation to stress; carotenoids; chlorophyll; non-photochemical quenching; photosystem 2; quantum yield; senescence.--Prolonged water-logging of rice plants is a major problem in rice growing countries. The work conducted so far to understand the mechanism of damage and adaptation of photosynthetic apparatus of green plants experiencing flooding stress (FS) is meager (Mauchamp and Methy 2004, Panda et al. 2006). The photosynthetic apparatus, especially its photosystem (PS) 2 is very sensitive to different abiotic stresses (Strasser and Tsimilli-Michael 2001, 2006, Deo et al. 2006. The degree of stress induced damage and stress adaptations can therefore be examined by monitoring the behaviour of the photosystem exposed to FS.Most of the studies related to stress have been conducted in developing or mature leaves with scant regard to investigate the stress response in senescence phase. This is why we examined the photosynthetic response of leaves of a local cultivar of rice plant subjected to FS. The primary leaves of intact rice seedlings experiencing flooding stress were used for measurement of PS2 photochemistry during senescence to examine the stress induced damage of the photosystem and the possible adaptation mechanism the leaves develop to counter the stress effect.FS was imposed by growing the rice seedlings on soil in submerged conditions as described by Panda et al. (2006). Rice (Oryza sativa L. cv. Swarna) seedlings were grown in 0.15×0.15 m pots with soil from the rice field around the campus of Sambalpur University for 7 d in continuous "white fluorescent light" (60 μmol m -2 s -1 ) at 25±2 ºC in the laboratory. One set of 7-d-old seedlings was completely submerged in a thermocol tank of depth 0.6 m till 15 th d whereas another set kept without submergence was taken as control. The photosynthetic response of plants to FS was studied during senescence (11-15 d) phase. In all cases the primary leaves of the rice seedlings were used for experiments. Pigments were extracted from leaves with chilled 80 % acetone. Estimation of chlorophyll (Chl) was performed spectrophotometrically as per Arnon (1949) and that of carotenoids as per Liaaen-Jensen and Jensen (1971). Total protein extracted from leaves was estimated following the method of Lowry et al. (1951). Chloroplasts from primary leaves of rice seedlings were isolated and 2,6-dichlorophenol indophen...

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Water stress-sensitized photoinhibition in senescing cotyledons of clusterbean: Changes in thylakoid structures and inactivation of photosystem 2

Cited by 12 publications

References 41 publications

Factors altering the membrane fluidity of spinach thylakoid as determined by fluorescence polarization

Factors altering the membrane fluidity of spinach thylakoid as determined by fluorescence polarization

Biochemical and morphophysiological strategies of Myracrodruon

Response of senescing rice leaves to flooding stress

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