Water–Surfactant Contact Studied by19F–1H Heteronuclear Overhauser Effect Spectroscopy

Raulet, R.; Furó, István; Brondeau, J.; Diter, B.; Canet, Daniel

doi:10.1006/jmre.1998.1487

Cited by 22 publications

(18 citation statements)

References 28 publications

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“…Intermolecular cross‐relaxation has been widely used to examine solvent interactions with peptides and proteins8, 28 and small molecules 29, 30. The interactions between solute and solvent components in our HFA–water mixture potentially could be so weak that the dipolar interactions leading to an NOE are describable in terms of random encounters of solute and solvent molecules; many such weak interactions were demonstrated for melittin dissolved in 35% HFIP–water 14.…”

Section: Discussionmentioning

confidence: 99%

Structure and solvation of melittin in hexafluoroacetone/water

Gerig

2004

Biopolymers

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Intermolecular (1)H[(19)F] and (1)H[(1)H] nuclear Overhauser effects have been used to explore interaction of solvent components with melittin dissolved in 50% hexafluoroacetone trihydrate (HFA)/water. Standard nuclear Overhauser effect experiments and an analysis of C(alpha)H proton chemical shifts confirm that the conformation of the peptide in this solvent is alpha-helical from residues Ala4 to Thr11 and from Leu13 to Arg24. The two helical regions are not collinear; the interhelix angle (144 +/- 20 degrees ) found in this work is near that observed in the solid state and previous NMR studies. Intermolecular NOEs arising from interactions between spins of the solvent and the solute indicate that both fluoroalcohol and water molecules are strongly enough bound to the peptide that solvent-solute complexes persist for > or =2 ns. Preferential interactions of HFA with many hydrophobic side chains of the peptide are apparent while water molecules appear to be localized near hydrophilic side chains. These results indicate that interactions of both HFA and water are qualitatively different from those present when the peptide is dissolved in 35% hexafluoro-2-propanol/water, a chemically similar helix-supporting solvent system.

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Section: Discussionmentioning

confidence: 99%

Structure and solvation of melittin in hexafluoroacetone/water

Gerig

2004

Biopolymers

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“…On the basis of the 19 F assignment, the HETCOR experiment clearly provides the C2, C4, and C6-C8 assignments and resolves, in the form of small but clearly measurable chemical shift differences, the ambiguity imposed on us by the F3-F5 overlap. 44 Unfortunately, the C3 and C4 resonances overlap; the full 13 C spectral assignment is given in Figure 1. This assignment is given further credibility by the same pattern (F3-F5 and C3-C4 overlaps) detected in perfluoroheptanoic acid.…”

Section: Methodsmentioning

confidence: 99%

Order Parameter Profile of Perfluorinated Chains in a Micelle

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Sitnikov

1999

Langmuir

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“…2; note that the spectrum of the nematic phase has been recorded under homonuclear decoupling (without decoupling, the spectrum is around 16 kHz wide (18)). Both the assignment (25) and the explanation of the apparent chemical shift differences between the two phases (17) are as previously communicated. The cross relaxation among the various 19 F spins along the perfluoroalkyl chain is rather fast (25), in particularly at low temperatures where the orientational fluctuation of the micelles is slower.…”

Section: Resultsmentioning

confidence: 96%