Water temperature induces jaw deformity and bone morphogenetic proteins (BMPs) gene expression in golden pompano Trachinotus ovatus larvae

Abstract

Golden pompano Trachinotus ovatus larvae were kept at 26, 29 and 33 °C for 15 days from 3-day post hatching (DPH) to 18 DPH to test temperature-dependent growth and jaw malformation. The growth, survival, jaw deformity and the gene expressions of bone morphogenetic proteins (BMPs) were used as criteria to examine the fish response to temperature manipulation. The growth rate of fish at 29 or 33 °C was significantly faster than fish at 26 °C, while fish survival at 29 °C was significantly higher than fish at 33 °C. Jaw deformity was significantly affected by water temperature. The highest jaw deformity occurred on fish at 33 °C, and the lowest jaw deformity was at 26 °C. The expressions of all BMP genes except BMP10 were significantly affected by water temperature. The highest gene expression of BMP2 was on fish at 29 °C, and the lowest expression was at 33 °C. For the BMP4 gene, the highest and lowest expressions were found on fish at 33 and 26 °C, respectively. The present study indicates that jaw deformity of golden pompano larvae increases with increasing temperature, and the gene expression of BMP4 proteins coincides with high jaw deformity and water temperature elevation.

Effects of different artificial motile activating media on sperms motility of Waigieu seaperch Psammoperca waigiensis throughout a reproductive season

“…The optimal temperatures for the maximum sperm motility of key farmed fish of the tropical regions such as tiger grouper, barramundi, red snapper, pompano, and cobia are 28-30℃, which is similar to the optimal range for their growth (Glencross & Felsing, 2006;Le et al, 2011Le et al, , 2014Le et al, , 2020Ma et al, 2016). In the current study, we found that MOT, VAP, and DSM were highest at 30℃ which is consistent across three periods during the spawning season.…”

Hydroxytyrosol-rich extract from olive juice as an additive in gilthead sea bream juveniles fed a high-fat diet: Regulation of somatic growth

“…A positive relationship between bmp4 expression and ossification has been established in different groups of vertebrates including fish ( Chen et al, 2002 ; Albertson et al, 2005 ). bmp4 is expressed in osteoblasts prior to formation of mineralized bone nodules being indicative of new bone formation, although high levels of expression have been also reported in response to elevated temperatures, and linked to increased jaw malformations ( Ytteborg et al, 2010 ; Ma et al, 2016 ). Indeed, bmp4 has been shown to play a role in jaw development ( Albertson et al, 2005 ) and more recently, in the determination of intermuscular bone distribution ( Su and Dong, 2018 ).…”

Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus)

“…On 10 day, the expression of BMP2, BMP4, and BMP5 genes were all upregulated, also indicating that HT induced the development of vertebral cartilage. The studies of Ma et al (2016a) also show that the increase of water temperature increased the expression of BMPs and resulted in an increase in the rate of jaw deformity in T. ovatus larvae. At the later stage of the experiment (30 days), the expressions of BMPs were relatively down-regulated in the HT group, which may be related to the adaptability of fish to water temperature.…”

“…Abnormality of bone results in a bottleneck in the culture of T. ovatus. The effects of temperature and nutrition factors on the skeletal deformities of T. ovatus, and the expression of some genes related to skeletal development in T. ovatus has also been studied (Ma et al, 2016a(Ma et al, , 2017a(Ma et al, ,b,c, 2018Yang et al, 2016), but the regulatory mechanism of temperature on the occurrence of vertebral fusion in juvenile T. ovatus is still not clear, while histopathological studies on the skeleton of T. ovatus are lacking. The objective of the present study was to elucidate the possible molecular reaction of vertebral fusion in juvenile T. ovatus under high water temperature combined with the histopathology.…”