Water transport in epididymal and ejaculated rhesus monkey (Macaca mulatta) sperm during freezing

Alapati, Raghava; Goff, Kelly; Kubisch, H. Michael; Devireddy, Ram V.

doi:10.1016/j.cryobiol.2008.07.005

Cited by 13 publications

(2 citation statements)

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“…A broad tolerance of macaque sperm to conditions required for cryopreservation has also been noted in studies of other sperm parameters such as osmotic stress, water permeability of membranes at room and freezing Effect of LDL and HDL on rhesus monkey sperm QX Dong et al 462 temperatures, and addition of cryoprotectants. [16][17][18] Differences in optimal LDL concentrations for various species could be due to their difference in composition of the sperm plasma membranes. 9 Because LDL consists of 86%-89% lipids and 12.5% proteins, 19 lipids have been the main focus for the study of the protective role of egg yolk.…”

Section: Discussionmentioning

confidence: 99%

“…When samples were frozen without permeable cryoprotectant, LDL in a concentration range of 10%-30% yielded similar post-thaw motility (33%-40%) and AMI (17)(18)(19)(20)(21)(22)(23)(24) as that of samples frozen in 20% egg yolk alone (39%67% for motility and 2469 for AMI). However, LDL at concentrations lower than 8% reduced post-thaw motility and AMI when compared with controls at 20% egg yolk (P,0.05; Figure 2).…”

Section: Evaluation Of Ldl Concentrationmentioning

confidence: 94%

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The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

Dong¹,

Rodenburg²,

Hill³

et al. 2011

Asian J Androl

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Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The present study evaluates their effect in comparison with egg yolk with or without the addition of permeable cryoprotectant (glycerol) on sperm cryopreservation of rhesus macaques. In addition, various additives intended to change the lipid composition of LDL-sperm membrane complex have also been tested for their effectiveness in preserving post-thaw viability. Our findings indicated that LDL is the main component in egg yolk that is responsible for its protective role for sperm cryopreservation in rhesus monkeys. Regardless of the presence or absence of glycerol, the protective role of LDL is similar to that of egg yolk and we did not observe any superiority in post-thaw survival with LDL when compared to egg yolk. Modifying the lipid composition of LDL-sperm membrane complex with the addition of cholesterol, cholesterol loaded cyclodextrin and phosphatidylcholine also did not yield any improvements in post-thaw survival; while addition of methyl-b-cyclodextrin reduced post-thaw motility. HDL plays a neutral role in sperm cryopreservation of rhesus monkeys. The present study suggests that egg yolk may still hold advantages when compared with LDL as effective components in extenders for sperm cryopreservation in rhesus monkeys.

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