Waterlogging tolerance, phenolic and flavonoid contents, and genetic diversity among Moringa oleifera self-pollinated lines

Nguyen, Bao Le Quy; Chanthanousone, Hatsadong; Ho, Han Ngoc; Ho, Nhi Thi Hoang; Le, Minh Hoang Duy; Rasphone, Sonexay; Nguyen, Co Quang; Truong, Hai Thi Hong

doi:10.1016/j.sajb.2023.04.012

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Individuals of the short capsule moringa phenotype were grouped in a sector of smaller dispersion indicating a greater similarity in genetic structure, while in the long capsule Moringa phenotype, a wide separation is observed between individuals of the long capsule phenotype, indicating a greater difference in genetic structure. This type of behavior is observed in different genetic identi cation and population variation studies, where one of the important factors is the presence and absence of genes derived from one or multiple common ancestors, and the crossbreeding or isolation of these genes generated by development and evolution in different geographical conditions (Natarajan & Joshi, 2015;Nguyen et al, 2023).…”

Section: Genetic Diversitymentioning

confidence: 90%

Genetic and cytogenetic analysis of Moringa oleifera short and long capsule phenotypes

Gómez-Martínez,

García-Ortiz,

Gómez-Martínez

et al. 2024

Preprint

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Cytogenetic and molecular studies hold significant importance in plant breeding programs. In the case of moringa, such studies are scarce. Therefore, the objective of this research was to determine the chromosomal number and genetic diversity parameters in two phenotypes of Moringa oleifera (short and long capsule) using chromosomal analysis and ISSR markers, respectively. Cytogenetic analyses were conducted using the "Somatic chromosomes in root apices" technique, with acetocarmine and papain for staining. It was possible to identify 2N = 28 chromosomes in a single cell of the long capsule phenotype, ranging in size from 0.05 to 0.10 µm. Cells in both moringa phenotypes were observed in prophase, metaphase, anaphase, and telophase. High genetic variability was found in both phenotypes of moringa, as indicated by a Shannon index of 0.81. Additionally, the principal component analysis and UPGMA groups revealed genetic isolation between the studied moringa phenotypes. The significant polymorphism obtained with most primers suggests that the tested ISSR markers are highly useful for studying genetic diversity in moringa.

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Section: Genetic Diversitymentioning

confidence: 90%

Genetic and cytogenetic analysis of Moringa oleifera short and long capsule phenotypes

Gómez-Martínez,

García-Ortiz,

Gómez-Martínez

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Genetic and cytogenetic analysis of Moringa oleifera short and long capsule phenotypes

Gómez-Martínez,

García-Ortiz,

Gómez-Martínez

et al. 2025

Reproduction and Breeding

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First Report of Pepper Yellow Leaf Curl Thailand Virus Infecting Hot Chili in Central Vietnam and Laos

Nguyen,

Rasphone,

Truong

et al. 2024

Plant Disease

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Hot chili pepper (Capsicum annuum) cultivation has been on the rise in South East Asia to meet export demands. In Thailand, the top chili exporter in South East Asia, chili production has been severely hampered by pepper yellow leaf curl disease (YLCD) caused by the begomovirus pepper yellow leaf curl Thailand virus (PepYLCThV) (Chiemsombat et al., 2018; Suwor et al., 2021). In the neighbouring countries of Laos and Vietnam, a limited survey of chili fields (200 plants in total) in Savannakhet (Savannakhet University campus, n = 150), Laos and Quang Nam province (Ka Dang commune, Dong Giang district, n = 50), central Vietnam in 2023 led to the finding of eight plants (5 in Laos and 3 in Vietnam) exhibiting YLCD-like symptoms, which included bright yellow color in young leaves and leaf curl and mosaic chlorosis in mature leaves (Fig. S1). Total DNA was extracted from leaves of two symptomatic plants (one from Savannakhet and one from Quang Nam) using a cetyltrimethylammonium bromide-based DNA extraction protocol (Doyle & Doyle, 1987; Nguyen et al., 2023). Next, PCR were performed using newly designed PepYLCThV-specific primers based on PepYLCThV sequences in GenBank (Table 1). PCR products of expected sizes were observed in samples with disease symptoms, but not from DNA extracted from C. annuum (cv. VA.99999) grown at the Institute of Biotechnology in Thua Thien Hue, Vietnam (Fig. S2). The amplicons were Sanger sequenced (Apical Scientific, Selangor, Malaysia) and the complete bipartite genome sequence of two isolates (‘Sava01’ from Laos and ‘QNam01’ from Vietnam) were obtained. The sequences of the DNA-A component from isolates ‘Sava01’ (GenBank PP437580) and ‘QNam01’ (GenBank PP437581) exhibited the highest sequence identity of 99.2% and 94.7% with the PepYLCThV isolate ‘ChiangDaoS1’ (GenBank OM677627), respectively (Table 2). Conversely, the sequences of the DNA-B component from the isolates ‘Sava01’ (GenBank PP437579) and ‘QNam01’ (GenBank PP437582) exhibited the highest similarity of 91.8% and 90.9% with the PepYLCThV isolate ‘KKN601’ (GenBank MW715820), respectively (Table 2). These results confirmed the presence of PepYLCThV in hot chili pepper plants exhibiting YLCD-like symptoms in central Vietnam and Laos. Infectious clones of PepYLCThV DNA-A and DNA-B (isolate ‘QNam01’) were created based on the pLX-AS vector as described by Pasin (2022), and transformed into Agrobacterium tumefaciens EHA105. The resulting bacteria were cultured in LB broth containing rifampicin (25 μg/mL) and kanamycin (50 μg/mL) at 28°C and used for agroinoculation of Nicotiana benthamiana (n = 6) and C. annuum (cv. VA.99999, n = 6) (4-6 leaf plants) as described by Pasin (2022). In all N. benthamiana plants, agroinoculation with both DNA-A and DNA-B infectious clones caused stunted growth, severe leaf curl, with yellow and white patches 21 days post inoculation (Fig. S3). In C. annuum plants, symptom expression, which included leaf curl and stunted leaves with yellow mosaic patterns, was observed in two out of six inoculated plants six weeks postinoculation (Fig. S3). PCR assays confirmed the presence of PepYLCThV DNA in N. benthamiana and C. annuum symptomatic leaves (Fig. S4). To our knowledge, this is the first report of pepper yellow leaf curl Thailand virus in hot chili pepper in Laos and central Vietnam. Appropriate containment and management strategies should be developed and implemented to control the spread of this disease in hot chili pepper crops in both countries.

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Waterlogging tolerance, phenolic and flavonoid contents, and genetic diversity among Moringa oleifera self-pollinated lines

Cited by 3 publications

References 19 publications

Genetic and cytogenetic analysis of Moringa oleifera short and long capsule phenotypes

Genetic and cytogenetic analysis of Moringa oleifera short and long capsule phenotypes

Genetic and cytogenetic analysis of Moringa oleifera short and long capsule phenotypes

First Report of Pepper Yellow Leaf Curl Thailand Virus Infecting Hot Chili in Central Vietnam and Laos

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