Watermelon chlorotic stunt virus (WmCSV): a serious disease threatening watermelon production in Jordan

Al‐Musa, A.; Anfoka, Ghandi; Al-Abdulat, A.; Misbeh, Samar; Ahmed, Fatima Haj; Otri, I.

doi:10.1007/s11262-011-0594-8

Cited by 18 publications

(20 citation statements)

References 37 publications

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“…Leaf samples collected from Jenin and Qalqilia were found to be mixed infected with WmCSV-[PAL] and SLCV-[PAL] (48%). Mixed infections with two or three whitefly transmitted viruses were also very common in cucurbits fields in Lebanon and Jordan [16,24]. Moreover, recent results have demonstrated that co-infection of melon plants with both WmCSV and SLCV results in a synergistic reaction characterized by severe symptoms and a major yield reduction [6,39].…”

Section: Resultsmentioning

confidence: 99%

“…DNA-A contains five ORFs, which encode for proteins involved in replication, regulation of gene expression, and encapsidation, while DNA-B contains two ORFs, encoding for proteins involved in viral movement and symptom development [7,8]. Begomoviruses have spread rapidly among cucurbit crops throughout the world, and such invasions were caused by Squash leaf curl virus (SLCV) [6,9,10,11], Cucurbit leaf curl virus (CuLCV) [12], Melon chlorotic leaf curl virus (MCLCuV) [13,14] and Watermelon chlorotic stunt virus (WmCSV) [6,15,16,17,18]. …”

Section: Introductionmentioning

confidence: 99%

“…Outside of the Red Sea region, the virus has been found in Iran [15,17], later on, between the years 2010–2012, the virus was reported in Israel, Jordan, Lebanon and Oman [6,16,24,25]. The complete genomes of two WmCSV isolates, from Sudan and from Iran, have been cloned and sequenced [15].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine

Ali‐Shtayeh¹,

Jamous²,

Mallah³

et al. 2014

Viruses

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The incidence of watermelon chlorotic stunt disease and molecular characterization of the Palestinian isolate of Watermelon chlorotic stunt virus (WmCSV-[PAL]) are described in this study. Symptomatic leaf samples obtained from watermelon Citrullus lanatus (Thunb.), and cucumber (Cucumis sativus L.) plants were tested for WmCSV-[PAL] infection by polymerase chain reaction (PCR) and Rolling Circle Amplification (RCA). Disease incidence ranged between 25%–98% in watermelon fields in the studied area, 77% of leaf samples collected from Jenin were found to be mixed infected with WmCSV-[PAL] and SLCV. The full-length DNA-A and DNA-B genomes of WmCSV-[PAL] were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis of virus genomes showed that DNA-A and DNA-B had 97.6%–99.42% and 93.16%–98.26% nucleotide identity with other virus isolates in the region, respectively. Sequence analysis also revealed that the Palestinian isolate of WmCSV shared the highest nucleotide identity with an isolate from Israel suggesting that the virus was introduced to Palestine from Israel.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine

Ali‐Shtayeh¹,

Jamous²,

Mallah³

et al. 2014

Viruses

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“…Tomato yellow leaf curl virus (TYLCV) has a monopartite genome [29] while that of Watermelon chlorotic stunt virus (WmCSV) is bipartite with a DNA-A and a DNA-B genomic component [11]. Both cause serious crop diseases: WmCSV threatens the production of cucurbit crops like watermelon ( Citrullus lanatus ), melons ( Cucumis melo ), and pumpkin ( Cucurbita moschata ) in North Africa, Yemen, Israel, Jordan and Iran [12]. Disease symptoms include vein yellowing, chlorotic mottling and stunting and severe curling of leaves with drastic reduction of yield [13], [14].…”

Section: Introductionmentioning

confidence: 99%

Quantification and Localization of Watermelon Chlorotic Stunt Virus and Tomato Yellow Leaf Curl Virus (Geminiviridae) in Populations of Bemisia tabaci (Hemiptera, Aleyrodidae) with Differential Virus Transmission Characteristics

2014

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Bemisia tabaci (Gennadius) is one of the economically most damaging insects to crops in tropical and subtropical regions. Severe damage is caused by feeding and more seriously by transmitting viruses. Those of the genus begomovirus (Geminiviridae) cause the most significant crop diseases and are transmitted by B. tabaci in a persistent circulative mode, a process which is largely unknown. To analyze the translocation and to identify critical determinants for transmission, two populations of B. tabaci MEAM1 were compared for transmitting Watermelon chlorotic stunt virus (WmCSV) and Tomato yellow leaf curl virus (TYLCV). Insect populations were chosen because of their high and respectively low virus transmission efficiency to compare uptake and translocation of virus through insects. Both populations harbored Rickettsia, Hamiltonella and Wolbachia in comparable ratios indicating that endosymbionts might not contribute to the different transmission rates. Quantification by qPCR revealed that WmCSV uptake and virus concentrations in midguts and primary salivary glands were generally higher than TYLCV due to higher virus contents of the source plants. Both viruses accumulated higher in insects from the efficiently compared to the poorly transmitting population. In the latter, virus translocation into the hemolymph was delayed and virus passage was impeded with limited numbers of viruses translocated. FISH analysis confirmed these results with similar virus distribution found in excised organs of both populations. No virus accumulation was found in the midgut lumen of the poor transmitter because of a restrained virus translocation. Results suggest that the poorly transmitting population comprised insects that lacked transmission competence. Those were selected to develop a population that lacks virus transmission. Investigations with insects lacking transmission showed that virus concentrations in midguts were reduced and only negligible virus amounts were found at the primary salivary glands indicating for a missing or modified receptor responsible for virus attachment or translocation.

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“…Sequence analysis revealed that the diseases were caused by the native Watermelon chlorotic stunt virus and Squash leaf curl virus Abudy et al, 2010;Ali-Shtayeh et al, 2014;Idris et al, 2006). WmCSV was first identified in Yemen (Jones et al, 1988) and after that, in the following years, it was reported through the Middle East and also in North Africa as the following: Sudan (Kheyr-Pour et al, 2000), Jordan (Al-Musa et al, 2011), Oman (Khan et al, 2012) and Palestine (Ali-Shtayeh et al, 2014). The symptoms caused by WmCSV are chlorotic mottling, vein yellowing, stunting and severe decrease of yield, generally in watermelon (Citrullus species) crops.…”

Section: Introductionmentioning

confidence: 99%

Cucurbit yellow stunting disorder virus and watermelon chlorotic stunt virus induced gene silencing in tobacco plants

Soliman¹,

Rezk²,

Alhudaib³

2018

Afr. J. Biotechnol.

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Cucurbit yellow stunting disorder virus (CYSDV) and Watermelon chlorotic stunt virus (WmCSV) are the most widespread and damaging viruses to cucurbits in the Middle East. CYSDV and WmCSV are cucurbit-infecting bipartite whitefly-transmitted begomoviruses. Post-transcriptional gene silencing (PTGS) is a universal mechanism by which plants are able to systemically switch off the expression of targeted genes via the reduction of steady-state levels of specific RNAs. PTGS was used in this study to control the two viruses. In this study, the efficiency of the dsRNA for the ability to trigger resistance against the CYSDV and WmCSV was investigated. Three regions of three genes of CYSDV genome were selected; the coat protein gene (CP), heat shock gene (Hsp70) and ORF3, while the two regions of two genes of WmCSV genome were selected; CP gene and rep gene. Bioassay, dot-blot hybridization and polymerase chain reaction (PCR) methods were capable to evaluate the resistance against viruses. Clear symptoms on tobacco plants took two to three weeks to appear and all non-infiltrating tobacco plants (positive control) showed viral symptoms after inoculation. Most of the agro-infiltrating sense/antisense constructs did not yield symptoms of the viruses. Dot-blot hybridization, showed that negative hybridization was obtained with infiltrating tobacco plants with prepared constructs compared to those non-infiltrating tobacco plants used as the control. Only one out of five gave positive signals with the construct pasCYSDV-Hsp70. Using PCR, positive reactions of the expected size of 500 bp fragment with WmCSV and 800 bp with CYSDV were obtained with the infiltrating tobacco plants with sense constructs, which pointed out the existence of viral genome in challenging tobacco plants. Infiltrating tobacco plants with sense/antisense constructs gave negative PCR pointed out the lack of the viral genome.

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Watermelon chlorotic stunt virus (WmCSV): a serious disease threatening watermelon production in Jordan

Cited by 18 publications

References 37 publications

Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine

Molecular Characterization of Watermelon Chlorotic Stunt Virus (WmCSV) from Palestine

Quantification and Localization of Watermelon Chlorotic Stunt Virus and Tomato Yellow Leaf Curl Virus (Geminiviridae) in Populations of Bemisia tabaci (Hemiptera, Aleyrodidae) with Differential Virus Transmission Characteristics

Cucurbit yellow stunting disorder virus and watermelon chlorotic stunt virus induced gene silencing in tobacco plants

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