Background Waterpipe smoking has become a global public health problem as its popularity increased over time, especially among youth. This study aims to examine waterpipe smoking patterns of university students in Ankara, Turkey, and compare the patterns in public and private universities, which have different economic and social environments. Students in these two types of universities differ, on average, in socioeconomic background and access to financial resources. Methods The survey was conducted among public (n=2685) and private (n=2485) university students via an online questionnaire. Logistic regressions (on the full sample and on the sample that excludes students who initiate before age 18) were used to test the association between ever using waterpipe and respondent characteristics such as age, gender, university type, living arrangements, and source of income. Descriptive statistics on consumption pattern (age at first use, use within last month, location of use, availability of waterpipe venues, whether it is usually shared, reasons for use, and harm perceptions) were also generated. Moreover, never, ever, and current user prevalence rates by age at first use were estimated for waterpipe and cigarettes. Results69% of students in private and 59% of students in public universities (p<0.001) were ever smokers of waterpipe. Being older, male, private university student, living alone or having roommate(s), as well as financial resources such as having access to a car, having income from work or family were associated with ever using waterpipe. Although more than half (68-70%) of ever users tried waterpipe before age 18, risk continued in university and 41-46% of those who tried before 18 were current users in university. For women who had abstained from waterpipe until university age, the risk of waterpipe use was higher in private than in public universities. ConclusionsWaterpipe smoking has increased and is highly common among university students. Students with higher socioeconomic status are at higher risk. There is an urgent need to implement the new creative interventions based on the WHO Framework Convention on Tobacco Control to prevent the young people from waterpipe smoking after 18 years old.