Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.

Abstract

Culture Collection of the University of Gøteborg, Gøteborg, SwedenComparative 16S rDNA sequence analysis indicates that two distinct sublineages, with a sequence dissimilarity of >4 % (bootstrap value, 100 %), exist within the genus Ralstonia: the Ralstonia eutropha lineage, which comprises Ralstonia basilensis, Ralstonia campinensis, R. eutropha, Ralstonia gilardii, Ralstonia metallidurans, Ralstonia oxalatica, Ralstonia paucula, Ralstonia respiraculi and Ralstonia taiwanensis; and the Ralstonia pickettii lineage, which comprises Ralstonia insidiosa, Ralstonia mannitolilytica, R. pickettii, Ralstonia solanacearum and Ralstonia syzygii comb. nov. (previously Pseudomonas syzygii ). This phylogenetic discrimination is supported by phenotypic differences. Members of the R. eutropha lineage have peritrichous flagella, do not produce acids from glucose and are susceptible to colistin, in contrast to members of the R. pickettii lineage, which have one or more polar flagella, produce acid from several carbohydrates and are colistin-resistant. Members of the R. pickettii lineage are viable for up to 6 days on tryptic soy agar at 25 6C, whereas members of the R. eutropha lineage are viable for longer than 9 days. It is proposed that species of the R. eutropha lineage should be classified in a novel genus, Wautersia gen. nov. Finally, based on the literature and new DNA-DNA hybridization data, it is proposed that Pseudomonas syzygii should be renamed Ralstonia syzygii comb. nov. INTRODUCTIONThe genus Ralstonia (Yabuuchi et al., 1995) was created to accommodate bacteria from ecologically diverse niches that were classified previously as Burkholderia (Yabuuchi et al., 1992) and Alcaligenes. The type species of the genus -Ralstonia pickettii (type strain, ATCC 27511 T ) -was regarded originally as the only representative of clinical importance (Fass & Barnishan, 1976;Fujita et al., 1981;Kahan et al., 1983;Gardner & Shulman, 1984;Verschraegen et al., 1985;Roberts et al., 1990a;Lacey & Want, 1991; Dimech et al., 1993;Raveh et al., 1993) In a previous study , it was indicated that species of the genus Ralstonia can be separated clearly into two phenotypically and genotypically distinct groups. Here, it is proposed to consolidate these findings by allocating one group of species to a novel genus, Wautersia The results of lactose and maltose acidification by Ralstonia pickettii strains, the 16S rRNA gene signature sequences of the Ralstonia species, detailed phenotypic data of different species and a similarity matrix of the 16S rDNA sequences are available as supplementary material (Tables A1, A2, A3 and A4, respectively) in IJSEM Online.

A case of newborn with community acquired pneumonia caused by Cupriavidus pauculus

“…In 1999, C. pauculus was firstly described as Ralstonia paucula; the description was identical to the one given by Vaneechoutte et al in 2004 as Wautersia paucula, and it was finally renamed C. pauculus. C. pauculus is an oxidase, catalase, citrate, and urea positive motile and non-fermentatile bacillus (7,8). This bacillus can be distinguished from R. pickettii, R. gilardii, and R. mannitolilytica by biochemical tests (nitrate reduction, carbohydrate acidification, urease, and fatty acid profile) and conclusively by DNA sequencing.…”

“…C. pauculus is the species most likely to be isolated (6). In 1999, C. pauculus was firstly described as Ralstonia paucula; the description was identical to the one given by Vaneechoutte et al in 2004 as Wautersia paucula, and it was finally renamed C. pauculus. C. pauculus is an oxidase, catalase, citrate, and urea positive motile and non-fermentatile bacillus (7,8).…”

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

“…Zhang, et al [40]; Nouri, et al [41] and Vaneechoutte, et al [42] also reported R. solanacearum to be negative in starch hydrolysis. These results can be attributed to the absence of some exoenzymes (amylases) by R.solanacearum [43].…”

Microbiological Characterization of the International Space Station Water Processor Assembly External Filter Assembly S/N 01

“…4 Cupriavidus metallidurans has the ability to survive in millimolar concentrations of heavy metals. 5 Comamonas testosteroni can accumulate poly-hydroxybutyrate in the cell. 6 Burkholderia multivorans has been known to colonize the lungs of cyctic fibrosis patients.…”