Wavelength dependence of biological damage induced by UV radiation on bacteria

Santos, A. L. Q.; Oliveira, Vanessa; Baptista, Inês; Henriques, Isabel; Gomes, Newton C. M.; Almeida, Adelaide; Correia, António; Cunha, Ângela

doi:10.1007/s00203-012-0847-5

Cited by 209 publications

(165 citation statements)

References 65 publications

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“…These results suggest that UVC irradiation could induce ROS production in rats and lead to perturbation of prooxidant and antioxidant balance, in which the oxidant amount was outweighing the antioxidant defense. These findings are corroborated by the study of Santos et al in which UVC was able to induce the generation of ROS, lipid peroxidation (TBARS), DNA damage, and reduced SOD enzyme activity 28 . According to this finding and its regression analysis, the authors suggested that biological damage was induced by UV irradiation on bacteria is wavelength dependent and revealed that inactivation of cellular bacteria by UVC was caused by the increase in ROS level together with DNA damage.…”

Section: Discussionsupporting

confidence: 73%

Administration of Purwoceng (<I>Pimpinella alpina</I> Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Spargue-Dawley Male Rats

Nasihun

Widayati

2016

JNR

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Ipomoea reniformis Chaos is claimed in Indian traditional medical practice to be useful in the treatment of epilepsy and neurological disorders. In the present study, pretreatment effect of methanolic extract of Ipomoea reniformis on epilepsy and psychosis was evaluated in rodents using standard procedures. Besides evaluating epileptic and behavioral parameters, neurotransmitters such as Gamma-Amino Butyric Acid (GABA) in epilepsy and in psychosis dopamine, noradrenaline and serotonin contents in the rodent brain were estimated. The extract pretreatment reduced maximal electro shock; Isoniazid (INH) and Pentylenetetrazole (PTZ) induced seizures and also significantly inhibited the attenuation of brain GABA levels by INH and PTZ in mice. These results suggested that the observed beneficial effect in epilepsy may be by enhancing the GABAergic system. The test drug also inhibited the apomorphine induced climbing and stereotyped behavior and showed significantly reduced levels of brain dopamine, noradrenaline and serotonin which may be due to blocking of central dopaminergic, noradrenergic and serotonergic pathways or by enhancing the GABAergic system. The results obtained in present study suggest that the title plant possesses antiepileptic and antipsychotic activities in rodents.

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Section: Discussionsupporting

confidence: 73%

Administration of Purwoceng (<I>Pimpinella alpina</I> Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Spargue-Dawley Male Rats

Nasihun

Widayati

2016

JNR

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“…, and H 2 O 2 were increased in dose dependent manner. It was supported by study reported by Santos AL in which irradiation with UVA, UVB, or UVC on bacteria increased in ROS production and DNA double strand breaks was occurred in dose dependent trend 32 .…”

Section: Discussionsupporting

confidence: 54%

Pimpinella alpina Molk Administration is Capable of Increasing Antioxidant and Decreasing Prooxidant Level following UVB Irradiation

Nasihun

Widayati

2018

Journal of Natural Remedies

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“…Viability is lost rapidly due to damage to one or more critical cellular components, but other cellular components are left intact. Examples include shortwave UV damage to DNA (25), inactivation of the respiratory chain by solar disinfection (26,27), denaturation of critical catabolic or metabolic machinery by low-temperature pasteurization (28), and inhibition of DNA, RNA, or protein synthesis by antibiotics that bind tightly to specific enzymatic targets within one of these pathways. In these situations, bacterial cells can remain visibly intact and impermeable to PMA and PI for hours or days after viability is lost.…”

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confidence: 99%

Dead or Alive: Molecular Assessment of Microbial Viability

Cangelosi

Meschke

2014

Appl Environ Microbiol

264

207

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Nucleic acid-based analytical methods, ranging from species-targeted PCRs to metagenomics, have greatly expanded our understanding of microbiological diversity in natural samples. However, these methods provide only limited information on the activities and physiological states of microorganisms in samples. Even the most fundamental physiological state, viability, cannot be assessed cross-sectionally by standard DNA-targeted methods such as PCR. New PCR-based strategies, collectively called molecular viability analyses, have been developed that differentiate nucleic acids associated with viable cells from those associated with inactivated cells. In order to maximize the utility of these methods and to correctly interpret results, it is necessary to consider the physiological diversity of life and death in the microbial world. This article reviews molecular viability analysis in that context and discusses future opportunities for these strategies in genetic, metagenomic, and single-cell microbiology.Yet it hath happened that the veritable body without the spirit hath walked.-Ambrose Bierce, The Death of Halpin Frayser M icrobiologists, like characters in zombie fiction, quickly learn the critical importance of distinguishing the living from the dead. In addition to characterizing the numbers and species of microorganisms in samples, it is important to collect data on their physiological states. The most fundamental physiological state of microbial cells is their viability, defined here as the capacity to form progeny. For ecologists, pathobiologists, metagenomicists, food or water safety analysts, infectious-disease clinicians, and virtually every other stripe of microbiologist, the observation of a viable microorganism in a sample means something entirely different from the observation of a dead one. Despite its importance, this distinction remains extremely challenging by current microbiological methods.Microbiological culture meets this requirement, as it selectively detects viable organisms. However, because only a small percentage of species can be cultured, this strategy underestimates microbial diversity (1-6). In contrast, nucleic acid-based methods, ranging from species-specific PCR to metagenomic methods, have greatly advanced our ability to detect diverse microorganisms independently of microbiological culture (7,8). However, these methods provide only limited information on the physiology of microorganisms in samples. They can assess microbial viability retrospectively, by measuring quantitative changes over time, but they cannot discern viability cross-sectionally (in single measurements). Traditional PCR is notoriously poor at differentiating DNA associated with a viable bacterial cell from DNA associated with an inactivated one or from a free DNA fragment. All of these analytes register as "hits" in PCR, despite their very distinct meanings.In order to address this limitation, alternative PCR-based strategies have been developed. This article reviews two complementary strategies. One strategy, term...

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Wavelength dependence of biological damage induced by UV radiation on bacteria

Cited by 209 publications

References 65 publications

Administration of Purwoceng (<I>Pimpinella alpina</I> Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Spargue-Dawley Male Rats

Administration of Purwoceng (<I>Pimpinella alpina</I> Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Spargue-Dawley Male Rats

Pimpinella alpina Molk Administration is Capable of Increasing Antioxidant and Decreasing Prooxidant Level following UVB Irradiation

Dead or Alive: Molecular Assessment of Microbial Viability

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