Wavelength dependence of matrix-assisted laser desorption and ionization using a tunable mid-infrared laser

Yoshihashi-Suzuki, Sachiko; Sato, Izuru; Awazu, Kunio

doi:10.1016/j.ijms.2007.12.003

Cited by 9 publications

(16 citation statements)

References 30 publications

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“…On the other hand, the absorption peak at the wavelength of 6.6 µm was red‐shifted to 6.7 µm in the peak wavelength of the ion signal intensity. A similar tendency has been reported for vacuum IR‐MALDI in the same wavelength range . To explain the differences in the peak wavelengths in the ion signal intensity and the absorbance of the matrix, Hatakeyama et al have reported that the wavelength dependence of the ion signal intensity is similar to the absorption spectrum of the matrix in the gas phase rather than the solid or liquid phase .…”

Section: Resultssupporting

confidence: 65%

“…Previous studies have also observed a difference between the absorption peak wavelength of the IR matrix and the wavelength optimizing ionization efficiency . It is inferred that these mismatches between the optimal wavelength for ionization and the absorption peak wavelength are due to the peak shift induced by laser irradiation.…”

Section: Resultsmentioning

confidence: 87%

“…A similar tendency has been reported for vacuum IR-MALDI in the same wavelength range. [21,28] To explain the differences in the peak wavelengths in the ion signal intensity and the absorbance of the matrix, Hatakeyama et al have reported that the wavelength dependence of the ion signal intensity is similar to the absorption spectrum of the matrix in the gas phase rather than the solid or liquid phase. [29] They calculated the theoretical absorption peak wavelengths of some matrix molecules, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, IR-MALDI studies have been performed in also 6 μm-band with the use of a mid-IR tunable laser using difference-frequency generation (DFG). [19][20][21] These reports suggest that excitation of C¼O stretching mode with corresponding laser wavelength could be a promising approach for effective ionization of various biomolecules. [9,22] In addition, liquid water has an absorption peak corresponding to the O-H bending vibration at a wavelength of 6.07 μm.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, many organic compounds and solvents, including water, have characteristic absorption peaks near a wavelength of 6–7 µm, which corresponds to the molecular vibration modes for O–H, CO, CH 3 , and C ≡ N bonds. Recently, IR‐MALDI studies have been performed in also 6 µm‐band with the use of a mid‐IR tunable laser using difference‐frequency generation (DFG) . These reports suggest that excitation of CO stretching mode with corresponding laser wavelength could be a promising approach for effective ionization of various biomolecules .…”

Section: Introductionmentioning

confidence: 99%

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Atmospheric pressure laser desorption/ionization using a 6-7 µm-band mid-infrared tunable laser and liquid water matrix

et al. 2015

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Due to the characteristic absorption peaks in the IR region, various molecules can be used as a matrix for infrared matrix-assisted laser desorption/ionization (IR-MALDI). Especially in the 6-7 µm-band IR region, solvents used as the mobile phase for liquid chromatography have absorption peaks that correspond to their functional groups, such as O-H, C=O, and CH3. Additionally, atmospheric pressure (AP) IR-MALDI, which is applicable to liquid-state samples, is a promising technique to directly analyze untreated samples. Herein we perform AP-IR-MALDI mass spectrometry of a peptide, angiotensin II, using a mid-IR tunable laser with a tunable wavelength range of 5.50-10.00 µm and several different matrices. The wavelength dependences of the ion signal intensity of [M + H](+) of the peptide are measured using a conventional solid matrix, α-cyano-4-hydroxycinnamic acid (CHCA) and a liquid matrix composed of CHCA and 3-aminoquinoline. Other than the O-H stretching and bending vibration modes, the characteristic absorption peaks are useful for AP-IR-MALDI. Peptide ions are also observed from an aqueous solution of the peptide without an additional matrix, and the highest peak intensity of [M + H](+) is at 6.00 µm, which is somewhat shorter than the absorption peak wavelength of liquid water corresponding to the O-H bending vibration mode. Moreover, long-lasting and stable ion signals are obtained from the aqueous solution. AP-IR-MALDI using a 6-7 µm-band IR tunable laser and solvents as the matrix may provide a novel on-line interface between liquid chromatography and mass spectrometry.

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Section: Resultssupporting

confidence: 65%

Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Atmospheric pressure laser desorption/ionization using a 6-7 µm-band mid-infrared tunable laser and liquid water matrix

et al. 2015

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Current literature in mass spectrometry

2008

J. Mass Spectrom.

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WJ, De Voe DL, Lee CS. Mass spectrometry-based tissue proteomics for cancer biomarker discovery. Pers Med 2007; 4: 45. Bohme DK. Gaseous ions and chemical mass spectrometry. Can J Chem 2008; 86: 177. Bollen G. Ion trap mass spectrometry of rare isotopes. Acta Phys Pol B 2008; 39: 433. Boonen K, Landuyt B, Baggerman G, Husson SJ, Huybrechts J, Schoofs L. Peptidomics: The integrated approach of MS, hyphenated techniques and bioinformatics for neuropeptide analysis. J Sep Sci 2008; 31: 427. Brouwers EEM, Tibben M, Rosing H, Schellens JHM, Beijnen JH. The application of inductively coupled plasma mass spectrometry in clinical pharmacological oncology research. Mass Spectrom Rev 2008; 27: 67. Harvey DJ. Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update covering the period 2001-2002. Mass Spectrom Rev 2008; 27: 125. Hou XL, Roos P. Critical comparison of radiometric and mass spectrometric methods for the determination of radionuclides in environmental, biological and nuclear waste samples. Anal Chim Acta 2008; 608: 105. Larsson L. Use of mass spectrometry for determining microbial toxins in indoor environments. J Environ Monit 2008; 10: 301. Mondello L, Tranchida PQ, Dugo P, Dugo G. Comprehensive two-dimensional gas chromatography-mass spectrometry: A review. Mass Spectrom Rev 2008; 27: 101. Mueller LN, Brusniak MY, Mani DR, Aebersold R. An assessment of software solutions for the analysis of mass spectrometry based quantitative proteomics data. J Proteome Res 2008; 7: 51. . ISOLTRAP: An on-line Penning trap for mass spectrometry on short-lived nuclides. Eur Phys J A 2008; 35: 1. Sutherland BW, Toews J, Kast J. Special feature: Perspective -Utility of formaldehyde cross-linking and mass spectrometry in the study of protein-protein interactions. J Mass Spectrom 2008; 43: 699.

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Theoretical analysis of correlation between ionization threshold fluence in IR‐MALDI and IR absorption spectrum of matrix molecules

Hatakeyama

Mashiko

Hazama

et al. 2012

Int J of Quantum Chemistry

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To investigate the correlation between the wavelength dependence of ionization threshold fluence of target molecule in matrix-assisted laser desorption/ionization by infrared (IR) laser and the IR absorption spectrum of matrix molecule, we have analyzed the IR absorption spectra of four matrix molecules using density functional theory and correlated ab initio molecular orbital method. The calculated IR absorption spectra of the isolated molecules showed more qualitative correlation with the wavelength dependence of ionization threshold fluence than those of the solid state structures. We can consider that a portion of matrix molecules lost the ordered crystal structure and that the transition to the diluted or isolated state occurred at the early process of IR laser irradiation.

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Wavelength dependence of matrix-assisted laser desorption and ionization using a tunable mid-infrared laser

Cited by 9 publications

References 30 publications

Atmospheric pressure laser desorption/ionization using a 6-7 µm-band mid-infrared tunable laser and liquid water matrix

Atmospheric pressure laser desorption/ionization using a 6-7 µm-band mid-infrared tunable laser and liquid water matrix

Current literature in mass spectrometry

Theoretical analysis of correlation between ionization threshold fluence in IR‐MALDI and IR absorption spectrum of matrix molecules

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