Wavelength dependence of the refractive index of human colorectal tissues: comparison between healthy mucosa and cancer

Carvalho, Sónia; Gueiral, Nuno; Nogueira, E.; Henrique, Rui; Oliveira, Luís; Tuchin, Valery V.

doi:10.18287/jbpe16.02.040307

Cited by 32 publications

(22 citation statements)

References 23 publications

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“…7, the experimental RI values for 820.8 and 850.7 nm are a little higher than the calculated fitting curve. As in the case of colon mucosa tissues that we have previously studied, 37,38 the increase in the RI at these wavelengths is evidence of lipid content in liver. Such evidence of lipids in human liver is not surprising since liver is reported to accumulate lipids.…”

Section: Ri Dispersion For Human Liversupporting

confidence: 55%

“…The knowledge of free/bound water content is also necessary for many other clinical applications, including tissue cryoprotection 41,42 and cancer diagnostics. 11,37,38,43 The OCAs are also mostly cryogenic agents and often used for cryoprotection of living tissues. Their strong osmotic properties and low-temperature freezing abilities are needed to prevent formation of ice crystals in order to keep tissue undamaged at low temperatures.…”

Section: Possible Applications Of Received Datamentioning

confidence: 99%

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Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue

et al. 2017

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The optical dispersion and water content of human liver were experimentally studied to estimate the optical dispersions of tissue scatterers and dry matter. Using temporal measurements of collimated transmittance [Tc(t)] of liver samples under treatment at different glycerol concentrations, free water and diffusion coefficient (Dgl) of glycerol in liver were found as 60.0% and 8.2×10-7 cm2/s, respectively. Bound water was calculated as the difference between the reported total water of 74.5% and found free water. The optical dispersion of liver was calculated from the measurements of refractive index (RI) of tissue samples made for different wavelengths between 400 and 1000 nm. Using liver and water optical dispersions at 20°C and the free and total water, the dispersions for liver scatterers and dry matter were calculated. The estimated dispersions present a decreasing behavior with wavelength. The dry matter dispersion shows higher RI values than liver scatterers, as expected. Considering 600 nm, dry matter has an RI of 1.508, whereas scatterers have an RI of 1.444. These dispersions are useful to characterize the RI matching mechanism in optical clearing treatments, provided that [Tc(t)] and thickness measurements are performed during treatment. The knowledge of Dgl is also important for living tissue cryoprotection applications.

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Section: Ri Dispersion For Human Liversupporting

confidence: 55%

Section: Possible Applications Of Received Datamentioning

confidence: 99%

Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue

et al. 2017

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“…Various optical methods widely used for tissue characterization, such as visible and near-infrared (NIR) spectroscopy, optical coherence tomography, and fluorescence spectroscopy, need exact data for RI of tissue, blood, and their components to quantify properly experimental data. [5][6][7][8][9][10][11] At present, the usage of RI as a diagnostic marker is urgent. [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] Sometimes, it is a self-sufficient parameter for tissue and blood characterization.…”

Section: Introductionmentioning

confidence: 99%

“…9 The RI of tissue was used as a marker for cancer, reflecting changes of optical properties in the course of pathology development. 10,11,[14][15][16][17][18][19][20] An additional motivation for this study is a lot of discrepancies between the RIs reported in the literature by different research groups.…”

Section: Introductionmentioning

confidence: 99%

Measurement of refractive index of hemoglobin in the visible/NIR spectral range

Lazareva

Tuchin

2018

J. Biomed. Opt.

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This study is focused on the measurements of the refractive index of hemoglobin solutions in the visible/near-infrared (NIR) spectral range at room temperature for characteristic laser wavelengths: 480, 486, 546, 589, 644, 656, 680, 930, 1100, 1300, and 1550 nm. Measurements were performed using the multiwavelength Abbe refractometer. Aqua hemoglobin solutions of different concentrations obtained from human whole blood were investigated. The specific increment of refractive index on hemoglobin concentration and the Sellmeier coefficients were calculated.

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“…RI of a tissue and its components is an optical parameter playing a fundamental role in light propagation in and interaction with tissues and cells as well as becoming an internal diagnostic parameter allowing for label free "optical histology." 17,[84][85][86][87][88] Over the last decades, various techniques to determine RI of biological tissues were developed; they include confocal microscopy, 17,89 optical fiber cladding method, 90 minimal deviation angle method, 91,92 OCT with multiple modifications, 17,[93][94][95][96][97][98][99][100][101][102] total internal reflection method, 88,[102][103][104][105][106] measurement the intensity profile of diffuse light refracted into the prism around the critical angle, 107 spectroscopic refractometry, 108 and polarizinginterference microscopy. 109 The RIs of different biological tissues and cell structures can be found in Ref.…”

Section: Introductionmentioning

confidence: 99%

Measurement of tissue optical properties in the context of tissue optical clearing

et al. 2018

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Nowadays, dynamically developing optical (photonic) technologies play an ever-increasing role in medicine. Their adequate and effective implementation in diagnostics, surgery, and therapy needs reliable data on optical properties of human tissues, including skin. This paper presents an overview of recent results on the measurements and control of tissue optical properties. The issues reported comprise a brief review of optical properties of biological tissues and efficacy of optical clearing (OC) method in application to monitoring of diabetic complications and visualization of blood vessels and microcirculation using a number of optical imaging technologies, including spectroscopic, optical coherence tomography, and polarization- and speckle-based ones. Molecular modeling of immersion OC of skin and specific technique of OC of adipose tissue by its heating and photodynamic treatment are also discussed.

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Wavelength dependence of the refractive index of human colorectal tissues: comparison between healthy mucosa and cancer

Cited by 32 publications

References 23 publications

Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue

Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue

Measurement of refractive index of hemoglobin in the visible/NIR spectral range

Measurement of tissue optical properties in the context of tissue optical clearing

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