Wavelength Dependence of Water Microdroplet Shattering with Dual IR Lasers and Dye Sensitization

Sugiyama, Akinori; Nakajima, Atsushi

doi:10.1515/zpch-2013-0455

Cited by 1 publication

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“…Since fragmentation of covalent bonds is rarely observed in LILBID, we speak primarily of the dissociation of noncovalent bonds. The mechanism of laser energy transfer, which leads to desorption and laser-induced dissociation, are the subject of on-going research [26][27][28][29][30] . Nevertheless, past qualitative studies have shown that different amounts of laser energy transfer lead to differences in complex dissociation 12,13,31,32 .…”

Section: Openmentioning

confidence: 99%

LILBID laser dissociation curves: a mass spectrometry-based method for the quantitative assessment of dsDNA binding affinities

Young

Hense

Immer

et al. 2020

Sci Rep

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One current goal in native mass spectrometry is the assignment of binding affinities to noncovalent complexes. Here we introduce a novel implementation of the existing laser-induced liquid bead ion desorption (LILBID) mass spectrometry method: this new method, LILBID laser dissociation curves, assesses binding strengths quantitatively. In all LILBID applications, aqueous sample droplets are irradiated by 3 µm laser pulses. Variation of the laser energy transferred to the droplet during desorption affects the degree of complex dissociation. In LILBID laser dissociation curves, laser energy transfer is purposely varied, and a binding affinity is calculated from the resulting complex dissociation. A series of dsDNAs with different binding affinities was assessed using LILBID laser dissociation curves. The binding affinity results from the LILBID laser dissociation curves strongly correlated with the melting temperatures from UV melting curves and with dissociation constants from isothermal titration calorimetry, standard solution phase methods. LILBID laser dissociation curve data also showed good reproducibility and successfully predicted the melting temperatures and dissociation constants of three DNA sequences. LILBID laser dissociation curves are a promising native mass spectrometry binding affinity method, with reduced time and sample consumption compared to melting curves or titrations.

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